{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T08:10:26Z","timestamp":1742544626375,"version":"3.40.1"},"reference-count":0,"publisher":"Press of International Journal of Ophthalmology (IJO Press)","issue":"4","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Ophthalmol"],"published-print":{"date-parts":[[2025,4,18]]},"abstract":"<jats:p>AIM: To explore the impact of insulin-like growth factor-1 receptor \u03b1 (IGF-1R\u03b1) on the differentiation fate of optic-cup-derived retinal stem cells (OC-RSCs) into retinal ganglion cells (RGCs) in vitro.\nMETHODS: OC-RSCs were isolated from optic cups of rats on embryonic day 12.5, and high-purity OC-RSCs were obtained by conditioned culture and passage. Differentiation of OC-RSCs into RGCs under different serum concentrations was examined using flow cytometry, and the serum concentration with high interference with differentiation ratio was selected. Furthermore, the effect of blocking IGF-1R\u03b1 on the differentiation of OC-RSCs into RGCs was analyzed through immunocytochemistry and Western blotting.\nRESULTS: Immunohistochemical analysis revealed IGF-1R\u03b1 was highly expressed in rat embryos at day 12.5. OC-RSCs were isolated and purified, and high-purity OC-RSCs were obtained. When 2.5% serum was administered, the ratio of differentiated RGCs (Thy-1.1 positive) decreased significantly, and the results of immunoblotting also confirmed the blockade of IGF-1R\u03b1 reduced Thy-1.1 protein expression.\nCONCLUSION: IGF-1R\u03b1 blocking can reduce the differentiation of OC-RSCs into RGCs.<\/jats:p>","DOI":"10.18240\/ijo.2025.04.03","type":"journal-article","created":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T07:55:13Z","timestamp":1742543713000},"page":"582-589","source":"Crossref","is-referenced-by-count":0,"title":["Inhibition of IGF-1R\u03b1 affects the differentiation fate of rat optic cup-derived retinal stem cells to retinal ganglion cells in vitro"],"prefix":"10.18240","volume":"18","author":[{"name":"Southwest Hospital\/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China; Key Lab of Visual Damage and Regeneration &#x0026; Restoration of Chongqing, Chongqing 400038, China","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi-You","family":"Li","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hai-Wei","family":"Xu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Southwest Hospital\/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China; Key Lab of Visual Damage and Regeneration &#x0026; Restoration of Chongqing, Chongqing 400038, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yu-Xiao","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Southwest Hospital\/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China; Key Lab of Visual Damage and Regeneration &#x0026; Restoration of Chongqing, Chongqing 400038, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Yong","family":"Huang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"name":"Southwest Hospital\/Southwest Eye Hospital, Third Military Medical University (Army Medical University), Chongqing 400038, China; Key Lab of Visual Damage and Regeneration &#x0026; Restoration of Chongqing, Chongqing 400038, China","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7543","container-title":["International Journal of Ophthalmology"],"original-title":[],"link":[{"URL":"http:\/\/ies.ijo.cn\/en_publish\/2025\/4\/20250403.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,21]],"date-time":"2025-03-21T07:55:17Z","timestamp":1742543717000},"score":1,"resource":{"primary":{"URL":"http:\/\/ies.ijo.cn\/gjyken\/ch\/reader\/view_abstract.aspx?file_no=20250403&flag=1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,18]]},"references-count":0,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2025,4,18]]}},"URL":"https:\/\/doi.org\/10.18240\/ijo.2025.04.03","relation":{},"ISSN":["2222-3959","2227-4898"],"issn-type":[{"value":"2222-3959","type":"print"},{"value":"2227-4898","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,18]]}}}