{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:25:15Z","timestamp":1787023515407,"version":"build-2736575974"},"reference-count":0,"publisher":"Press of International Journal of Ophthalmology (IJO Press)","issue":"8","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Ophthalmol"],"published-print":{"date-parts":[[2025,8,18]]},"abstract":"<jats:p>AIM: To investigate the functions and potential mechanisms of obtusifolin in dry eye disease (DED) in a rat model.\nMETHODS: A rat DED model was established via topical administration of benzalkonium chloride (BAC), followed by administration of obtusifolin. Conjunctival irritation score and tear production were measured to evaluate DED symptoms. Enzyme-linked immunosorbent assay (ELISA) was employed for determining inflammatory cytokine levels in rat conjunctiva. Periodic acid-Schiff staining and corneal fluorescein staining were implemented for assessing goblet cell numbers and corneal epithelial defects, respectively. Western blotting showed zonula occludens-1 (ZO-1), matrix metalloproteinase-9 (MMP-9), and mitogen-activated protein kinase (MAPK)\/nuclear factor kappa B (NF-\u03baB) signaling-related protein levels in the conjunctiva.\nRESULTS: Topical application of obtusifolin alleviated conjunctival irritation and enhanced tear production in BAC-induced DED rats. Obtusifolin attenuated conjunctival inflammatory response and goblet cell loss as well as corneal epithelial barrier disruption in DED rats. Obtusifolin suppressed extracellular signal-regulated kinase (ERK), p38, and NF-\u03baB phosphorylation in the conjunctiva of DED rats.\nCONCLUSION: Obtusifolin ameliorates DED in rats possibly by alleviating inflammation via the inactivation of MAPK\/NF-\u03baB signaling.<\/jats:p>","DOI":"10.18240\/ijo.2025.08.02","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T08:37:11Z","timestamp":1752827831000},"page":"1426-1432","source":"Crossref","is-referenced-by-count":3,"title":["Obtusifolin ameliorates dry eye model in rats by reducing inflammation and blocking MAPK\/NF-\u03baB pathways"],"prefix":"10.18240","volume":"18","author":[{"name":"Department of Ophthalmology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061, Hubei Province, China; Affiliated Hospital of Hubei University of Chinese Medicine, Wuhan 430061, Hubei Province, China; Hubei Provincial Key Laboratory of Liver and Kidney Research and Application of Traditional Chinese Medicine, Wuhan 430061, Hubei Province, China","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Zhu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang-Chang","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Ophthalmology, Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan 430061, Hubei Province, China; Affiliated Hospital of Hubei University of Chinese Medicine, Wuhan 430061, Hubei Province, China; Hubei Provincial Key Laboratory of Liver and Kidney Research and Application of Traditional Chinese Medicine, Wuhan 430061, Hubei Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7543","container-title":["International Journal of Ophthalmology"],"original-title":[],"link":[{"URL":"http:\/\/ies.ijo.cn\/en_publish\/2025\/8\/20250802.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T08:37:12Z","timestamp":1752827832000},"score":1,"resource":{"primary":{"URL":"http:\/\/ies.ijo.cn\/gjyken\/ch\/reader\/view_abstract.aspx?file_no=20250802&flag=1"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,8,18]]},"references-count":0,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2025,8,18]]}},"URL":"https:\/\/doi.org\/10.18240\/ijo.2025.08.02","relation":{},"ISSN":["2222-3959","2227-4898"],"issn-type":[{"value":"2222-3959","type":"print"},{"value":"2227-4898","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,8,18]]}}}