{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T08:23:20Z","timestamp":1789115000530,"version":"build-2803163510"},"reference-count":0,"publisher":"Press of International Journal of Ophthalmology (IJO Press)","issue":"10","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Int J Ophthalmol"],"published-print":{"date-parts":[[2026,10,18]]},"abstract":"AIM: To investigate the functions of microRNA-26a-5p (miR-26a-5p) in the high glucose (HG)-induced rat retinal M\u00fcller cells (rMC-1) apoptosis and its possible mechanism.\nMETHODS: rMC-1 cells were transfected with miR-26a-5p mimics or phosphatase and tensin homolog (PTEN) siRNA under HG conditions. Cell apoptosis was analyzed by propidium iodide (PI) and annexin V fluorescein isothiocyanate (FITC) staining. PTEN\/phosphatidylinositol 3-kinase (PI3K)\/protein kinase B (Akt) pathway activation was assessed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Western blot. Inflammatory cytokines, interleukin (IL)-1\u03b2, IL-6, were measured by enzyme-linked immunosorbent assay (ELISA). The miR-26a-5p-PTEN interaction was validated by dual-luciferase assay.\nRESULTS: HG significantly suppressed miR-26a-5p expression and induced apoptosis in rMC-1 cells. Overexpression of miR-26a-5p attenuated HG-induced apoptosis by directly targeting PTEN, as confirmed by dual-luciferase reporter assay, which demonstrated that miR-26a-5p directly binds to the PTEN 3\u2019-UTR, resulting in a 50% reduction in luciferase activity (P<0.01). Consistent with this, miR-26a-5p overexpression downregulated PTEN expression, enhanced PI3K\/Akt phosphorylation, and suppressed the secretion of IL-1\u03b2 and IL-6 (all P<0.01).\nCONCLUSION: Upregulation of miR-26a-5p protects rMC-1 from HG-induced apoptosis by targeting PTEN via regulating the PI3K\/Akt pathway and inflammatory responses. miR-26a-5p may be a potential therapeutic target for diabetic retinal neurodegeneration.<\/jats:p>","DOI":"10.18240\/ijo.2026.10.06","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T07:25:34Z","timestamp":1789111534000},"page":"1929-1938","source":"Crossref","is-referenced-by-count":0,"title":["MicroRNA-26a-5p inhibits high glucose-induced M\u00fcller cells apoptosis by mediating PTEN\/PI3K\/Akt pathway"],"prefix":"10.18240","volume":"19","author":[{"name":"Department of Ophthalmology, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi Province, China","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"De-Rong","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Ophthalmology, Medical College of Ankang Vocational and Technical College (Ankang Central Hospital), Ankang 725000, Shaanxi Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Di","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Ophthalmology, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu-Wen","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"The Molecular Neuropharmacology Laboratory and the Eye-Brain Research Center, The State Key Laboratory of Ophthalmology, Optometry and Visual Science, Eye Hospital, Wenzhou Medical University, Wenzhou 325027, Zhejiang Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Bo","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"School of Basic Medical Sciences, Xi’an Medical University, Xi’an 710061, Shaanxi Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan-Dan","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Ophthalmology, Xi’an Aier Ancient City Eye Hospital, Xi’an 710018, Shaanxi Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao-Yu","family":"Gong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Ophthalmology, The First Affiliated Hospital of Xi’an Medical University, Xi’an 710005, Shaanxi Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"name":"Department of Ophthalmology, Shaanxi Provincial People’s Hospital, Xi’an 710068, Shaanxi Province, China","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7543","container-title":["International Journal of Ophthalmology"],"original-title":[],"link":[{"URL":"https:\/\/www.ijo.cn\/gjyken\/article\/pdf\/20261006","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T07:25:36Z","timestamp":1789111536000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.ijo.cn\/gjyken\/article\/abstract\/20261006"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,10,18]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2026,10,18]]}},"URL":"https:\/\/doi.org\/10.18240\/ijo.2026.10.06","relation":{},"ISSN":["2222-3959","2227-4898"],"issn-type":[{"value":"2222-3959","type":"print"},{"value":"2227-4898","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,10,18]]}}}