{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T00:14:49Z","timestamp":1717028089274},"reference-count":0,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Biochim Pol"],"abstract":"<jats:p>Cancer tissue obtained by biopsy or at surgery is usually handled and stored as formalin-fixed, paraffin-embedded (FFPE) blocks. While primarily prepared for histological examination, this material represents a valuable resource that is stable and transportable, and which is ideal for examination in 3 dimensions. It has been used for DNA and protein analysis but the potential for transcriptional profiling has been little exploited: the quality of the extracted RNA is uncertain and there are few reports of its efficient use. We review the background and practicalities of this approach and describe a pilot study as a worked example in which RNA of good yield and quality could be consistently obtained from differentiated areas of adrenocortical carcinoma (ACC) tissue.<\/jats:p>","DOI":"10.18388\/abp.2020_5611","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T19:46:36Z","timestamp":1653680796000},"source":"Crossref","is-referenced-by-count":1,"title":["Use of dissected paraffin block tissue as a source of mRNA for transcriptional profiling and biomarker identification: a review, with preliminary findings in adrenocortical carcinoma tissue"],"prefix":"10.3389","author":[{"given":"Urszula","family":"Waszut","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman  F.","family":"Taylor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2022,5,27]]},"container-title":["Acta Biochimica Polonica"],"original-title":[],"link":[{"URL":"https:\/\/ojs.ptbioch.edu.pl\/index.php\/abp\/article\/download\/5611\/5324","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ojs.ptbioch.edu.pl\/index.php\/abp\/article\/download\/5611\/5324","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T13:42:54Z","timestamp":1716990174000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontierspartnerships.org\/articles\/10.18388\/abp.2020_5611\/pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,27]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18388\/abp.2020_5611","relation":{},"ISSN":["1734-154X","0001-527X"],"issn-type":[{"value":"1734-154X","type":"electronic"},{"value":"0001-527X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,5,27]]}}}