{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T03:26:10Z","timestamp":1787023570432,"version":"build-2736575974"},"reference-count":0,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T00:00:00Z","timestamp":1669593600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Biochim Pol"],"abstract":"<jats:p>Objective: Cerebral ischemia\/reperfusion (I\/R) injury is stimulated by blood restoration after ischemic stroke. Inflammatory response and inflammasome activation exerted vital functions in the development of cerebral I\/R injury. miR-155-5p regulates inflammatory response in some diseases, while its role in inflammatory response and inflammasome activation of cerebral I\/R injury development is unclear. Hence, the research focuses on investigating if miR-155-5p attenuate cerebral I\/R injury via regulating inflammatory response and inflammasome activation and exploring the potential mechanism. Methods: The oxygen-glucose deprivation\/reoxygenation (OGD\/R) model and the middle cerebral artery occlusion (MCAO) model were constructed. Cell viability and cytotoxicity were reflected by CCK-8 assay and LDH activity. The inflammatory cytokines secretion was determined using ELISA assay. Brain tissue infarction was evaluated using TTC staining. Results: miR-155-5p, Thioredoxin Interacting Protein (TXNIP) and NLR Family Pyrin Domain Containing 3 (NLRP3) were highly expressed in OGD\/R model and MCAO rats. Knockdown of miR-155-5p alleviated cell injury, cell inflammation, and cell pyroptosis stimulated by OGD\/R. Besides, miR-155-5p regulated TXNIP\/NLRP3 pathway through modulating Dual-Specificity Phosphatase 14 (DUSP14) expression. Furthermore, knockdown of miR-155-5p improved brain tissue infarction and inhibited inflammation response and cell pyroptosis of MCAO rats. Conclusion: Knockdown of miR-155-5p attenuated I\/R inflammation and cell pyroptosis of cerebral via modulating DUSP14\/TXNIP\/NLRP3 pathway. These findings may provide a promising strategy to attenuate cerebral I\/R injury.<\/jats:p>","DOI":"10.18388\/abp.2020_6095","type":"journal-article","created":{"date-parts":[[2022,11,28]],"date-time":"2022-11-28T11:49:40Z","timestamp":1669636180000},"source":"Crossref","is-referenced-by-count":9,"title":["miR-155-5p accelerates cerebral ischemia-reperfusion inflammation injury and cell pyroptosis via DUSP14\/ TXNIP\/NLRP3 pathway"],"prefix":"10.3389","author":[{"given":"Yu","family":"Shi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhendong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ke","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4318-843X","authenticated-orcid":false,"given":"Ke","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1965","published-online":{"date-parts":[[2022,11,28]]},"container-title":["Acta Biochimica Polonica"],"original-title":[],"link":[{"URL":"https:\/\/ojs.ptbioch.edu.pl\/index.php\/abp\/article\/download\/6095\/5500","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ojs.ptbioch.edu.pl\/index.php\/abp\/article\/download\/6095\/5500","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T09:53:43Z","timestamp":1716976423000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontierspartnerships.org\/articles\/10.18388\/abp.2020_6095\/pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,11,28]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18388\/abp.2020_6095","relation":{},"ISSN":["1734-154X","0001-527X"],"issn-type":[{"value":"1734-154X","type":"electronic"},{"value":"0001-527X","type":"print"}],"subject":[],"published":{"date-parts":[[2022,11,28]]}}}