{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T05:46:34Z","timestamp":1747201594433,"version":"3.40.5"},"reference-count":0,"publisher":"Frontiers Media SA","license":[{"start":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T00:00:00Z","timestamp":1695081600000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Acta Biochim Pol"],"abstract":"<jats:p>This study aimed to figure out how microRNA (miR)-411-3p\u2019s impacts on methotrexate (MTX)\u2019s cellular uptake and cytotoxicity in acute lymphoblastic leukaemia (ALL) CEM-C1 cells by targeting Yin-yang 1 (YY1). miR-411-3p and YY1 were detected by RT-qPCR or Western blot. Intracellular MTX concentration was measured by enzyme-linked immunosorbent assay. Cell viability and apoptosis were evaluated by CCK-8, clonal formation assay, and flow cytometry. Verification of miR-411-3p and YY1\u2019s targeting link was manifested. It came out that miR-411-3p mimic or si-YY1 elevated intracellular MTX, MTX-induced cytotoxicity and apoptosis rate in CEM-C1. However, the inverse results were noticed in cells introduced with miR-411-3p inhibitor or oe-YY1. Meanwhile, it was found that cell relative luciferase activity was reduced after co-transfection of miR-411-3p mimic with YY1-WT, indicating that miR-411-3p targeted YY1. Elevation of YY1 could turn around elevating miR-411-3p\u2019s impacts on MTX\u2019s cellular uptake and cytotoxicity in CEM-C1 cells. These findings convey that miR-411-3p motivated MTX\u2019s cellular uptake and cytotoxic impacts via targeting YY1 in leukemia cells. This study is helpful for learning about the mechanisms underlying MTX responses in ALL patients.<\/jats:p>","DOI":"10.18388\/abp.2020_6242","type":"journal-article","created":{"date-parts":[[2023,9,19]],"date-time":"2023-09-19T11:51:48Z","timestamp":1695124308000},"source":"Crossref","is-referenced-by-count":0,"title":["MicroRNA-411-3p motivates methotrexate\u2019s cellular uptake and cytotoxicity via targeting Yin-yang 1 in leukemia cells"],"prefix":"10.3389","author":[{"given":"HuiJing","family":"Sun","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ShuGuang","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"ZhouSheng","family":"Yang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"MingYu","family":"Meng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Dai","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"XinYe","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8499-377X","authenticated-orcid":false,"given":"XiaoYu","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2023,9,19]]},"container-title":["Acta Biochimica Polonica"],"original-title":[],"link":[{"URL":"https:\/\/ojs.ptbioch.edu.pl\/index.php\/abp\/article\/download\/6242\/5827","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ojs.ptbioch.edu.pl\/index.php\/abp\/article\/download\/6242\/5827","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,29]],"date-time":"2024-05-29T13:46:25Z","timestamp":1716990385000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.frontierspartnerships.org\/articles\/10.18388\/abp.2020_6242\/pdf"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9,19]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18388\/abp.2020_6242","relation":{},"ISSN":["1734-154X","0001-527X"],"issn-type":[{"type":"electronic","value":"1734-154X"},{"type":"print","value":"0001-527X"}],"subject":[],"published":{"date-parts":[[2023,9,19]]}}}