{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T04:09:36Z","timestamp":1778126976509,"version":"3.51.4"},"reference-count":0,"publisher":"Frontiers Media SA","issue":"2","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Pharm Pharm Sci"],"abstract":"<jats:p>Purpose. A combined microextraction and separation method is presented for the determination of methadone in exhaled breath condensate (EBC) which is a promising non-invasive biological component for monitoring drug concentrations. Methods. In this work, dispersive liquid\u2013liquid microextraction (DLLME) and ultrasonic liquid\u2013liquid microextraction (ULLME) procedure coupled with a validated liquid chromatography method were used for analysis of methadone in EBC collected using an in-house cold trap setup. The method has been validated according to the FDA guidelines using EBC-spiked samples and tested on a number of EBC samples collected from patients. Results. The best DLLME conditions involved the use of a disperser solvent of methanol (1 mL), extraction solvent of chloroform (200 mL), EBC sample pH of 10.0 and centrifugation at 6000 rpm for 5 minutes. The conditions for ULLME were 150 mL of chloroform and the samples were sonicated for 4 minutes. The method was validated over the concentration range of 0.5\u201310 mg\/L-1 in EBC. Inter- and intra-day precision and accuracy were less than 5 % where the acceptable levels are less than 20%.\u00a0 Furthermore, the validated method was successfully applied for the determination of methadone in patients\u2019 EBC samples. Conclusions. The outcomes indicate that the developed LC-UV combined with DLLME and\/or ULLME extraction methods can be employed for the extraction and separation of methadone in EBC samples.\u00a0This article is open to POST-PUBLICATION REVIEW. Registered readers (see \u201cFor Readers\u201d) may comment by clicking on ABSTRACT on the issue\u2019s contents page.<\/jats:p>","DOI":"10.18433\/j3wk65","type":"journal-article","created":{"date-parts":[[2016,3,18]],"date-time":"2016-03-18T23:09:00Z","timestamp":1458342540000},"page":"207","source":"Crossref","is-referenced-by-count":21,"title":["Extraction and Analysis of Methadone in Exhaled Breath Condensate Using a Validated LC-UV Method"],"prefix":"10.3389","volume":"18","author":[{"given":"Maryam","family":"Khoubnasabjafari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Khalil","family":"Ansarin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vahid","family":"Jouyban-Gharamaleki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vahid","family":"Panahi-Azar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ali","family":"Shayanfar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laya","family":"Mohammadzadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abolghasem","family":"Jouyban","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1965","published-online":{"date-parts":[[2015,6,5]]},"container-title":["Journal of Pharmacy &amp; Pharmaceutical Sciences"],"original-title":[],"deposited":{"date-parts":[[2023,1,13]],"date-time":"2023-01-13T13:34:41Z","timestamp":1673616881000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.library.ualberta.ca\/jpps\/index.php\/JPPS\/article\/view\/23468"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,6,5]]},"references-count":0,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2015,4,20]]}},"URL":"https:\/\/doi.org\/10.18433\/j3wk65","relation":{},"ISSN":["1482-1826","1482-1826"],"issn-type":[{"value":"1482-1826","type":"electronic"},{"value":"1482-1826","type":"print"}],"subject":[],"published":{"date-parts":[[2015,6,5]]}}}