{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:48:01Z","timestamp":1791726481026,"version":"4.3.4"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":["kne-publishing.com","knepublishing.com","ajmb.org"],"crossmark-restriction":true},"short-container-title":["AJMB"],"abstract":"<jats:p>Background: L-Asparaginase is a crucial enzyme to treat Acute Lymphoblastic Leukemia (ALL), as it depletes L-asparagine, an essential amino acid for cancer cell survival. However, its clinical use is often restricted due to hypersensitivity reactions. This study examined the anti-proliferative effects and hypersensitivity of fungal L-aspara-ginases (L-ASNases) from Trichosporon asahii isolate ChL11 (TaIChL11 L-ASNase) and Candida palmioleophila isolate JK12 (CpIJK12 L-ASNase).\r\nMethods: The enzymes were produced and purified through ammonium sulfate precipitation, dialysis, and Sephadex G-100 chromatography, and tested on leukemia cells and BALB\/c female mice to assess immune responses.\r\nResults: TaIChL11 L-ASNase had a molecular weight of 40 kDa, Michaelis constant (KM) of 1.66\u00d710\u207b\u00b2 mM, and Vmax of 37.23 mM\/min, while CpIJK12 L-ASNase had a molecular weight of 135 kDa, KM of 2.3\u00d710\u207b\u00b2 mM, and Vmax of 14.03 mM\/min. Both enzymes exhibited significant anti-proliferative effects against CCRF-CEM cancer cells, with half-maximal inhibitory concentration (IC50) values of 2.74 U\/ml for TaIChL11 L-ASNase and 3.30 U\/ml for CpIJK12 L-ASNase after 48 hr, improving further after 72 hr. They also showed low cytotoxicity toward normal Vero E6 cells. in vivo studies demonstrated that TaIChL11 ASNase-treated mice had significantly lower Immunoglobulin (Ig) G levels than those treated with commercial L-ASNase from Erwinia chrysanthemi (Owenism) (p&lt;0.005), with no detectable IgE response.\r\nConclusion: These findings indicate that fungal L-ASNases, particularly TaIChL11 ASNase, with lower L-glutaminase activity and a favorable safety profile, could be promising alternatives to bacterial L-ASNases, potentially enhancing ALL treatment with fewer side effects.<\/jats:p>","DOI":"10.18502\/ajmb.v17i3.19275","type":"journal-article","created":{"date-parts":[[2025,7,31]],"date-time":"2025-07-31T18:32:38Z","timestamp":1753986758000},"update-policy":"https:\/\/doi.org\/10.18502\/knecrossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["Characterization and Evaluation of the Anti-proliferative Activity and Hypersensitivity  of L-Asparaginase from Trichosporon asahii Isolate ChL11 and Candida palmioleophila  Isolate JK12"],"prefix":"10.18502","author":[{"given":"Tekeba","family":"Sisay","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naomi","family":"Maina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victor","family":"Mobegi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabina","family":"Wachira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7770","published-online":{"date-parts":[[2025,7,31]]},"container-title":["Avicenna Journal of Medical Biotechnology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/AJMB\/article\/download\/19275\/17947","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/AJMB\/article\/download\/19275\/17947","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T14:39:30Z","timestamp":1771943970000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/AJMB\/article\/view\/19275"},"secondary":[{"URL":"https:\/\/www.ajmb.org\/Article?id=70616","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,31]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ajmb.v17i3.19275","relation":{},"ISSN":["2008-4625","2008-2835"],"issn-type":[{"value":"2008-4625","type":"electronic"},{"value":"2008-2835","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7,31]]}}}