{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T09:37:05Z","timestamp":1793093825145,"version":"4.5.0"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":["knepublishing.com"],"crossmark-restriction":true},"short-container-title":["AJMB"],"abstract":"<jats:p>Background: Lung cancer remains a major global health burden, and conventional therapies often fail to meet clinical needs. Although non-coding RNAs are increasingly recognized as key regulators in cancer, the role of NKILA and its modulation by the miR-103\/107 cluster in lung cancer growth and metastasis is not fully characterized. This study investigates the impact of the NKILA\u2013miR-103\/107 axis on lung cancer progression and metastatic behavior.\u00a0\r\nMethods: To suppress miR-103 and miR-107 activity in the human lung cancer cell line A549, specific LNA-based inhibitors were introduced into the cells. Following in vitro transfection with either miR-specific LNA or a mock control, alterations in the components of the NKILA\u2013miR-103\/107 regulatory pathway were quantified using quantitative real-time PCR. The effects of miR inhibition on cellular behavior were then evaluated by measuring A549 cell growth with an MTT assay and assessing invasive capacity through a Transwell invasion test.\u00a0\r\nResults: In lung cancer A549 cells, suppression of miR-103\/107 using LNA inhibitors led to a marked elevation in NKILA transcript levels. Functional assays showed that miR-103\/107 inhibition was associated with a significant decrease in A549 cell viability (p=0.0041) and a pronounced reduction in invasive capacity (p=0.001).\u00a0\r\nConclusion: In this in vitro study using A549 lung cancer cells, the NKILA\u2013miR-103\/107 axis was associated with lung cancer aggressiveness. miR-103\/107 inhibition upregulated NKILA and was accompanied by decreased proliferation and invasion of A549 cells. These findings are preliminary and limited to a single cell line model. Further validation in additional lung cancer models and in vivo systems is required to determine the broader biological and therapeutic relevance of this regulatory interaction.\u00a0<\/jats:p>","DOI":"10.18502\/ajmb.v18i3.22238","type":"journal-article","created":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T13:01:02Z","timestamp":1785934862000},"update-policy":"https:\/\/doi.org\/10.18502\/knecrossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["miR-103\/107\u2013Mediated Suppression of NKILA Enhances Oncogenic Behaviors in    Human Lung Cancer Cells"],"prefix":"10.18502","author":[{"given":"Davood","family":"Jafari","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Naghiloo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seyyed Shamsadin","family":"Athari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farshid","family":"Noorbakhsh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nima","family":"Rezaei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7770","published-online":{"date-parts":[[2026,8,5]]},"container-title":["Avicenna Journal of Medical Biotechnology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/AJMB\/article\/download\/22238\/20721","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/AJMB\/article\/download\/22238\/20721","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T09:15:03Z","timestamp":1793092503000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/AJMB\/article\/view\/22238"},"secondary":[{"URL":"https:\/\/submission.ajmb.org\/article_250464.html","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,5]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ajmb.v18i3.22238","relation":{},"ISSN":["2008-4625","2008-2835"],"issn-type":[{"value":"2008-4625","type":"electronic"},{"value":"2008-2835","type":"print"}],"subject":[],"published":{"date-parts":[[2026,8,5]]}}}