{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T13:00:24Z","timestamp":1780837224186,"version":"3.54.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["CJN"],"abstract":"<jats:p>Background: Chronic migraine (CM) is a debilitating neurological disorder often complicated by medication overuse (MO). While abobotulinumtoxinA (ABO-BoNT-A) is a well-established preventive treatment for CM, its molecular mechanisms are not fully understood. Emerging evidence suggests that neurotrophic tyrosine receptor kinase 2 (NTRK2), SRC kinase signaling inhibitor 1 (SRCIN1), and P2X4 purinergic receptor (P2X4R) are involved in migraine chronification and botulinum neurotoxin A (BoNT-A) function, but their roles in humans remain underexplored. This case series investigated changes in NTRK2, SRCIN1, and P2X4R gene expression in peripheral blood pre- and post-BoNT-A treatment and assessed associated clinical outcomes in patients.\r\nMethods: The messenger ribonucleic acid (mRNA) levels of NTRK2, SRCIN1, and P2X4R genes were analyzed in a sample of eight patients with CM and MO following BoNT-A treatment using quantitative real-time polymerase chain reaction (qRT-PCR). Additionally, migraine characteristics were assessed using Migraine Disability Assessment Scale (MIDAS), Headache Impact Test-6 (HIT-6), and Patient Health Questionnaire-9 (PHQ-9).\r\nResults: The intervention resulted in early measurable improvements in migraine symptoms and disability. Post-treatment, P2X4R expression significantly increased (P &lt; 0.05), while NTRK2 and SRCIN1 showed no significant changes.\r\nConclusion: Findings indicate that P2X4R upregulation may be linked to the therapeutic effects of BoNT-A, while NTRK2 and SRCIN1 appear uninvolved. The trend in P2X4R expression suggests it may serve as a predictive biomarker and therapeutic target, but further validation in larger cohorts is necessary.<\/jats:p>","DOI":"10.18502\/cjn.v24i3.21595","type":"journal-article","created":{"date-parts":[[2026,5,30]],"date-time":"2026-05-30T11:52:49Z","timestamp":1780141969000},"source":"Crossref","is-referenced-by-count":0,"title":["Changes in P2X4 receptor expression  following AbobotulinumtoxinA  treatment in patients with chronic migraine: A case series study"],"prefix":"10.18502","author":[{"given":"Zhale","family":"Salami","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saeed","family":"Irian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mansoureh","family":"Togha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elham","family":"Jafari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Delara","family":"Hazegh-Fetratjoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7770","published-online":{"date-parts":[[2026,5,26]]},"container-title":["Current Journal of Neurology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/CJN\/article\/download\/21595\/20035","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/CJN\/article\/download\/21595\/20035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,7]],"date-time":"2026-06-07T12:37:42Z","timestamp":1780835862000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/CJN\/article\/view\/21595"},"secondary":[{"URL":"https:\/\/cjn.tums.ac.ir\/article_40539.html","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,26]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/cjn.v24i3.21595","relation":{},"ISSN":["2717-011X"],"issn-type":[{"value":"2717-011X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5,26]]}}}