{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T05:33:05Z","timestamp":1734499985275,"version":"3.30.2"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJAAI"],"abstract":"<jats:p>Despite many years having passed since exposure to sulfur mustard (SM) gas, there are many exposed subjects who are still suffering from delayed pulmonary complications. The levels of pro-inflammatory cytokines in the lung of these subjects have not been investigated in delay phase. In this study, we evaluated mRNA expression of pro-inflammatory \u00a0cytokines (tumor necrosis factor alpha (TNF\u03b1), tumor necrosis factor receptor type 1 (TNFR1), and interleukin 1 beta (IL-1\u03b2) \u00a0) in lung biopsy of SM-exposed subjects and compared them with control (non-exposed) subjects. We used formalin-fixed, paraffin-embedded (FFPE) tissue for this purpose.\r\nLung FFPE blocks of SM-exposed subjects (30 samples) and a control group (30 samples) were collected from archival pathology department. The total mRNA of FFPE tissues were extracted and the mRNA expression of pro-inflammatory \u00a0cytokines were determined by quantitative Real Time PCR (RT-qPCR). The obtained results from two groups were compared to each other and non-parametric statistical analyses were carried out on them.\r\nOur studies showed that the mRNA expression of TNF\u03b1, TNFR1 and IL-1\u03b2 \u00a0in lung tissue of SM injured and control people have no significant difference (p-value= 0.159, 0.832 and 0.314 respectivly). TNFR1 showed direct correlation with TNF\u03b1 (r=0.867, p=0.002) and IL-1\u03b2 (r= 0.65, p=0.006).\r\nThe evaluation of mRNA expression in pro-inflammatory cytokines in lung of SM-exposed subjects after 20 years showed that these mediators are similar to those of non-exposed group and there was no acute inflammation in lung of these patients.<\/jats:p>","DOI":"10.18502\/ijaai.v17i4.97","type":"journal-article","created":{"date-parts":[[2018,10,15]],"date-time":"2018-10-15T12:38:27Z","timestamp":1539607107000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of mRNA Expression Levels of TNF\u03b1, TNFR1 and IL1\u03b2 in Lung Tissue 20 Years after Sulfur-mustard Exposure"],"prefix":"10.18502","author":[{"given":"Marzieh","family":"Eghtedardoost","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zuhair","family":"Mohammad Hassan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tooba","family":"Ghazanfari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alireza","family":"Sadeghipour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mostafa","family":"Ghanei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2018,10,15]]},"container-title":["Iranian Journal of Allergy, Asthma and Immunology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJAAI\/article\/download\/97\/36","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJAAI\/article\/download\/97\/36","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T15:26:30Z","timestamp":1734449190000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJAAI\/article\/view\/97"},"secondary":[{"URL":"http:\/\/ijaai.tums.ac.ir\/index.php\/ijaai\/article\/view\/1582","label":"UNIVERSITY"},{"URL":"http:\/\/publish.kne-publishing.com\/index.php\/IJAAI\/article\/view\/97","label":"PUBLISHER"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,10,15]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijaai.v17i4.97","relation":{},"ISSN":["1735-5249","1735-1502"],"issn-type":[{"type":"electronic","value":"1735-5249"},{"type":"print","value":"1735-1502"}],"subject":[],"published":{"date-parts":[[2018,10,15]]}}}