{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T13:28:45Z","timestamp":1772026125607,"version":"3.50.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJAAI"],"abstract":"<jats:p>Cytotoxic CD4+ T cells eliminate human cytomegalovirus (HCMV)-infected cells through direct cytotoxic granules exocytosis. We aimed to evaluate the functional cytotoxic gene profile of CD4+ T cells alongside the frequency of the cytotoxic phenotype in renal transplant recipients with cytomegalovirus reactivation.\r\nBlood samples were collected from twenty renal recipients with and without HCMV reactivation (HCMV+ and HCMV- groups) and ten healthy adults (control group). CD4+ T cells were isolated to assess the frequency of cytotoxic CD4+ T cells via CD107a surface staining using flow cytometry and to evaluate gene expression of perforin, granzyme B, Runt-related transcription factor 3 (RUNX3), and Eomesodermin (Eomes) by quantitative PCR.\r\nThe frequency of CD4+ CD107a+ T cells was higher in the HCMV+ group compared to the HCMV- group and significantly higher than in the control group (22.69\u2009\u00b1\u20093.47 vs 16.41\u2009\u00b1\u20092.24 and 11.60\u2009\u00b1\u20091.17, respectively). Perforin gene levels were reduced in the HCMV+ group compared to the other two groups, while granzyme B gene levels were similar between HCMV+ and HCMV- groups but lower than in the control group (0.63\u2009\u00b1\u20091.24 vs 0.67\u2009\u00b1\u20092.27 and 1.00\u2009\u00b1\u20090.00, respectively).\r\nThis study demonstrated an increased frequency of cytotoxic CD4+ T cells with potentially reduced functionality in kidney transplant patients with HCMV infection. It also suggests that these cells might employ other mechanisms, such as death receptor-mediated killing, or the production of other granules.\r\n\u00a0<\/jats:p>","DOI":"10.18502\/ijaai.v23i6.17376","type":"journal-article","created":{"date-parts":[[2024,12,29]],"date-time":"2024-12-29T03:37:55Z","timestamp":1735443475000},"source":"Crossref","is-referenced-by-count":0,"title":["Increased Cytotoxic CD4+ T Cells with Reduced Cytotoxic Gene Profile Expression in Cytomegalovirus Reactivated Kidney Transplant Patients"],"prefix":"10.18502","author":[{"given":"Yashgin","family":"Hassanzadeh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramin","family":"Yaghobi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parviz","family":"Pakzad","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bita","family":"Geramizadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2024,12,28]]},"container-title":["Iranian Journal of Allergy, Asthma and Immunology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJAAI\/article\/download\/17376\/16218","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJAAI\/article\/download\/17376\/16218","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T12:39:45Z","timestamp":1772023185000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJAAI\/article\/view\/17376"},"secondary":[{"URL":"https:\/\/ijaai.tums.ac.ir\/index.php\/ijaai\/article\/view\/4079","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,12,28]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijaai.v23i6.17376","relation":{},"ISSN":["1735-5249","1735-1502"],"issn-type":[{"value":"1735-5249","type":"electronic"},{"value":"1735-1502","type":"print"}],"subject":[],"published":{"date-parts":[[2024,12,28]]}}}