{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T12:27:29Z","timestamp":1762432049690,"version":"3.30.2"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJM"],"abstract":"<jats:p>Background and Objectives: Escherichia coli (E. coli) sequence type 131 (ST131) is associated with extended-spectrum beta-lactamase (ESBL) production and fluoroquinolone resistance. This study aimed to investigate the prevalence of ST131, ESBL, and plasmid-mediated quinolone resistance (PMQR) genes in the ciprofloxacin-resistant (CIPR ) and ESBL producers from women with UTI.\r\nMaterials and Methods: The CIP-resistant ESBL producing (CIPR \/ESBL+ ) E. coli isolates were screened for ST131-by specific PCR of mdh and gyrB. The ESBL and PMQR genes were screened by single PCR. The ST131 and non-ST131 isolates were selected to determine the mutations of gyrA and parC using PCR and sequencing, and also their genetic background by the Pasteur-MLST scheme.\r\nResults: Overall, 55% (33\/60) CIPR \/ESBL+ isolates were identified as ST131 (94% O25b-ST131). Resistance rate to ampicillin-sulbactam (70%), aztreonam (97%) and gentamicin (61%), the prevalence of aac(6\u2032)-Ib-cr (66%), blaCTX-M-15 (82%), the profile of qnrS+aac(6\u2032)-Ib-cr (30%), and the double mutation in the parC was significantly higher in ST131 than nonST131 isolates. The coexistence of PMQR and ESBL genes was found in more than 50% of ST131 and non-ST131 isolates. ST131 isolates differentiated into PST43 and PST506.\r\nConclusion: Management of women with UTI caused by the CIPR \/ESBL+ isolates (ST131) co-harbored PMQR, ESBL, and chromosomal mutations, is important for their effective therapy.<\/jats:p>","DOI":"10.18502\/ijm.v13i3.6389","type":"journal-article","created":{"date-parts":[[2021,6,15]],"date-time":"2021-06-15T12:38:02Z","timestamp":1623760682000},"source":"Crossref","is-referenced-by-count":4,"title":["Distribution of ciprofloxacin-resistance genes among ST131 and non-ST131 clones of Escherichia coli isolates with ESBL phenotypes isolated from women with urinary tract infection"],"prefix":"10.18502","author":[{"given":"Masoumeh","family":"Rasoulinasab","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fereshteh","family":"Shahcheraghi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Mehdi","family":"Feizabadi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahram","family":"Nikmanesh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Azade","family":"Hajihasani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Mehdi","family":"Aslani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2021,6,14]]},"container-title":["Iranian Journal of Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/18.184.16.47\/index.php\/IJM\/article\/download\/6389\/6115","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/18.184.16.47\/index.php\/IJM\/article\/download\/6389\/6115","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T16:32:52Z","timestamp":1734453172000},"score":1,"resource":{"primary":{"URL":"https:\/\/18.184.16.47\/index.php\/IJM\/article\/view\/6389"},"secondary":[{"URL":"https:\/\/ijm.tums.ac.ir\/index.php\/ijm\/article\/view\/2947","label":"UNIVERSITY"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/6389","label":"PUBLISHER"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,14]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijm.v13i3.6389","relation":{},"ISSN":["2008-4447","2008-3289"],"issn-type":[{"type":"electronic","value":"2008-4447"},{"type":"print","value":"2008-3289"}],"subject":[],"published":{"date-parts":[[2021,6,14]]}}}