{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,28]],"date-time":"2026-02-28T15:05:53Z","timestamp":1772291153640,"version":"3.50.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJM"],"abstract":"<jats:p>Background and Objectives: Acinetobacter baumannii (A. baumannii), an opportunistic pathogen, has been isolated from sewage, soil, and hospital wards.The prevalence of multidrug-resistance A. baumannii has seriously caused a health crisis in hospital settings. Bacteriophages have been used as an alternativetherapy for control carbapenem-resistant bacteria-caused infections. We aimed to assay lytic effect of a filamentous phage on clinical bacterial strains.\r\nMaterials and Methods: 500-ml water samples was collected from sewage in Tehran. Sewage samples were precipitated at 6000 rpm for 10 minutes andfiltered using 0.45-\u00b5m syringe filters. Bacteriophage was isolated using double-layer agar assay and evaluated its stability at various pH and temperatureranges. In addition, the stability of the phage was assayed at chloroform 0.1%.\r\nResults: Transmission electron microscopy imagine showed phage is filamentous called vB-AbaI-TMU2. The phage affect- ed on its own host so that couldnot effect on any Pseudomonas aeruginosa and Klebsiella pneumoniae strains. vB-AbaI- TMU2 phage was stable at pH 5 and 7 and also temperatures 25 and37\u00b0C. vB-AbaI-TMU2 was stable at chloroform 0.1%. vB-AbaI-TMU2 phage infected carbapenem-resistant A. baumannii strains while other bacterial strainswere resistant to.\r\nConclusion: The present study indicated the isolated phage had a narrow host range and is susceptible to various pH values and temperatures.<\/jats:p>","DOI":"10.18502\/ijm.v14i5.10966","type":"journal-article","created":{"date-parts":[[2022,10,25]],"date-time":"2022-10-25T07:46:47Z","timestamp":1666684007000},"source":"Crossref","is-referenced-by-count":2,"title":["Lytic potential of a filamentous bacteriophage isolated from sewage water in Tehran on clinical carbapenem-resistant strains"],"prefix":"10.18502","author":[{"given":"Keyvan","family":"Esmaeili-Fard-Barzegar","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahin","family":"Najar-Peerayeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramin","family":"Mazaheri Nezhad Fard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bita","family":"Bakhshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2022,10,24]]},"container-title":["Iranian Journal of Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/10966\/10342","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/10966\/10342","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T13:31:38Z","timestamp":1772112698000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/10966"},"secondary":[{"URL":"https:\/\/ijm.tums.ac.ir\/index.php\/ijm\/article\/view\/3677","label":"UNIVERSITY"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/10966","label":"PUBLISHER"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,24]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijm.v14i5.10966","relation":{},"ISSN":["2008-4447","2008-3289"],"issn-type":[{"value":"2008-4447","type":"electronic"},{"value":"2008-3289","type":"print"}],"subject":[],"published":{"date-parts":[[2022,10,24]]}}}