{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T12:09:28Z","timestamp":1772107768277,"version":"3.50.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJM"],"abstract":"<jats:p>Background and Objectives: Mesenchymal Stem Cells (MSCs) can repair gastrointestinal tract damage. The Secretome of MSCs has a high capacity to inhibit bacterial colonization and the subsequent inflammatory responses of Vibrio cholerae. Materials and Methods: The Caco-2 cells were treated with adipose-derived MSCs (AD-MSCs) secretome and then in- fected with V. cholerae. Subsequently, the bacterial attachment and invasion, cholera toxin gene expression, PGE2 and IL-6 secretion, TNF-\u03b1, IL-1\u03b2, and IL-8 expression, and apoptosis of Caco-2 cells were evaluated.\r\nResults: The secretome of AD-MSCs significantly reduced the V. cholerae attachment and internalization on Caco-2 epithe- lial cells (P&lt;0.0001). The cholera toxin (Ctx-B) gene expression (FR=4.56 \u00b1 0.66) and PGE2 production (P=0.0007) were also significantly reduced. The production of NO and TNF-\u03b1, IL-1\u03b2, and IL-8 pro-inflammatory cytokines were significantly (P&lt;0.05) reduced in exposure to the secretome of AD-MSCs. Secretome also improved a significant 81.33% increase in IL-6 production (128.1 \u00b1 37.6 pg\/mL) and showed a 12.36% significant decrease in epithelial cell apoptosis (P&lt; 0.0001) after exposure to V. cholerae.\r\nConclusion: The secretome of AD-MSCs can play a critical role in inhibiting bacterial colonization, and subsequent inflam- matory responses, and maintaining the integrity of the epithelial barrier. The secretome may be effective in the prevention of hypovolemic shock.<\/jats:p>","DOI":"10.18502\/ijm.v16i1.14875","type":"journal-article","created":{"date-parts":[[2024,2,12]],"date-time":"2024-02-12T07:07:06Z","timestamp":1707721626000},"source":"Crossref","is-referenced-by-count":1,"title":["Secretome of adipose derived-mesenchymal stem cells reduces the Vibrio cholerae attachment to Caco-2 cells and subsequent inflammatory responses"],"prefix":"10.18502","author":[{"given":"Alireza","family":"Moulazade","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sara","family":"Soudi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bita","family":"Bakhshi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2024,2,10]]},"container-title":["Iranian Journal of Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/14875\/14017","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/14875\/14017","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T11:24:34Z","timestamp":1772105074000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/14875"},"secondary":[{"URL":"https:\/\/ijm.tums.ac.ir\/index.php\/ijm\/article\/view\/4412","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,2,10]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijm.v16i1.14875","relation":{},"ISSN":["2008-4447","2008-3289"],"issn-type":[{"value":"2008-4447","type":"electronic"},{"value":"2008-3289","type":"print"}],"subject":[],"published":{"date-parts":[[2024,2,10]]}}}