{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T12:09:04Z","timestamp":1772107744388,"version":"3.50.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJM"],"abstract":"<jats:p>Background and Objectives: Leptospirosis is a zoonotic disease caused by pathogenic Leptospira serovars. The genus Leptospira cannot differentiated by conventional techniques. However, identity determination of pathogenic serovar is pre- cious of public health problems and epidemiological studies. Pulsed-field gel electrophoresis facilitates rapid identification of Leptospires to the serovar levels.\r\nMaterials and Methods: In this study, we employed PFGE to evaluate 28 Leptospira isolates, with animal, human and en- vironmental origin, obtained from Razi Vaccine and Serum Research Institute of Karaj, Iran. PFGE patterns of 28 Leptospira serovars were generated using the Not I restriction enzyme in comparison with the lambda ladder.\r\nResults: Out of 28 serovars evaluated, we identified 22 different pulsed types, designated P1- P22. Out of 22 pulse groups, 3 were found to be a common type, but others were a single Type. Groups consisting of the common type were P3, P9, P14, and P16. The results showed that the discriminatory index of PFGE by Not I enzyme was 0.99, demonstrating heterogeneous differentiation among serovar members.\r\nConclusion: The PFGE methodology used in this study showed excellent interlaboratory report usability, rapid, reliable, enabling standardization and data sharing between laboratories.<\/jats:p>","DOI":"10.18502\/ijm.v16i3.15763","type":"journal-article","created":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T11:52:25Z","timestamp":1719057145000},"source":"Crossref","is-referenced-by-count":0,"title":["Application of pulsed-field gel electrophoresis for molecular identification of pathogenic Leptospira species in Iran: a rapid and reliable method"],"prefix":"10.18502","author":[{"given":"Pejvak","family":"Khaki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohsen","family":"Bagherpour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Gharakhani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maryam Sadat","family":"Soltani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fereshteh","family":"Shahcheraghi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vajihe Sadat","family":"Nikbin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2024,6,19]]},"container-title":["Iranian Journal of Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/15763\/14757","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/15763\/14757","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T11:23:36Z","timestamp":1772105016000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/15763"},"secondary":[{"URL":"https:\/\/ijm.tums.ac.ir\/index.php\/ijm\/article\/view\/4293","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,19]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijm.v16i3.15763","relation":{},"ISSN":["2008-4447","2008-3289"],"issn-type":[{"value":"2008-4447","type":"electronic"},{"value":"2008-3289","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6,19]]}}}