{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T12:08:56Z","timestamp":1772107736736,"version":"3.50.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJM"],"abstract":"<jats:p>Background and Objectives: Rotavirus and Hepatitis A virus are responsible for causing gastroenteritis and jaundice. The current vaccination approaches have proven insufficient, especially in low-income countries. In this study, we presented a novel dual-vaccine candidate that combines the rotavirus VP8 protein and the hepatitis A virus VP1.\r\nMaterials and Methods: The VP8*-rotavirus+AAY+HAV-VP1 fusion protein was produced using an Escherichia coli expression system. The recombinant protein had a molecular weight of approximately 45.5 kDa and was purified through affinity chromatography. BALB\/c mice were injected subcutaneously with the recombinant protein, VP1, VP8 and vaccines for rotavirus and hepatitis A virus, both with and without ALUM and M720 adjuvants. ELISA assays were used to measure total IgG, IgG1, IgG2, and short-term and long-term IL-5 and IFN-\u03b3 responses.\r\nResults: The fusion protein, when combined with adjuvants, elicited significantly higher total IgG, IgG1, and IgG2 re- sponses compared to VP1 and VP8 alone, as well as the rotavirus and hepatitis A vaccines. Furthermore, it induced a higher short-term IL-5 and IFN-\u03b3 response while demonstrating a higher long-term IL-5 response compared to the rotavirus and hepatitis A vaccines.\r\nConclusion: This study demonstrates that the VP8*-rotavirus+AAY+HAV-VP1 fusion protein is a promising dual vaccine candidate for immunization against hepatitis A and rotaviruses.<\/jats:p>","DOI":"10.18502\/ijm.v16i3.15797","type":"journal-article","created":{"date-parts":[[2024,6,22]],"date-time":"2024-06-22T11:52:25Z","timestamp":1719057145000},"source":"Crossref","is-referenced-by-count":1,"title":["Designing the fusion protein of rotavirus VP8 and hepatitis A virus VP1 and evaluating the immunological response in BALB\/c mice"],"prefix":"10.18502","author":[{"given":"Hassan","family":"Yarmohammadi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammadreza","family":"Aghasadeghi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abbas Akhavan","family":"Sepahi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mojtaba","family":"Hamidi-fard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Golnaz","family":"Bahramali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2024,6,21]]},"container-title":["Iranian Journal of Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/15797\/14766","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/15797\/14766","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T11:23:11Z","timestamp":1772104991000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/15797"},"secondary":[{"URL":"https:\/\/ijm.tums.ac.ir\/index.php\/ijm\/article\/view\/4600","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,6,21]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijm.v16i3.15797","relation":{},"ISSN":["2008-4447","2008-3289"],"issn-type":[{"value":"2008-4447","type":"electronic"},{"value":"2008-3289","type":"print"}],"subject":[],"published":{"date-parts":[[2024,6,21]]}}}