{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T12:08:14Z","timestamp":1772107694551,"version":"3.50.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJM"],"abstract":"<jats:p>Background and Objectives:\u00a0Urinary tract infections are one of the world's major health problems. In addition, clinical disorders may result from the presence of bacteria or fungi in urine. The aim of this study was to isolate\u00a0Escherichia coli (E. coli)\u00a0strains from midstream urine samples, and to determine molecular characterization of encoded Extended Spectrum Beta-Lactamase (ESBL) genes.Materials and Methods:\u00a0Collected urine samples were streaked on MacConkey, blood and EMB agar plates, then identifying\u00a0E. coli\u00a0isolates by using antibiotic susceptibility tests. ESBL production was measured using double disc diffusion. Furthermore, uniplex PCR was performed to identify two ESBL genes (blaCTX\u00a0and\u00a0blaTEM).Results:\u00a0Among 412 isolates, 198 (48.1%) were\u00a0E. coli\u00a0strains, followed by\u00a0Staphylococcus saprophyticus,\u00a0Klebsiella\u00a0sp.,\u00a0Serratia\u00a0sp.,\u00a0Enterococci\u00a0sp. and\u00a0Proteus\u00a0sp. with frequency of 132 (32.0%), 51 (12.4%), 15 (3.6%), 10 (2.4%), and 6 (1.5%) respectively. Female participants who were between the ages of 40 and 49 years old, married, and pregnant were more likely to develop urinary tract infections (UTIs).\u00a0E. coli\u00a0species were present in 189 (95.5) of the recurrent UTIs. Regarding antimicrobial susceptibility testing of\u00a0E. coli\u00a0isolates, the highest percentage of resistance and susceptible rates were found for nalidixic acid (75.8%) and gentamicin (64.1%) respectively. Among the\u00a0E. coli\u00a0isolates, 25 (12.6%) were ESBL-producers. The\u00a0blaCTX-M\u00a0gene was genetically confirmed in 20 (10.1%) of the isolates.Conclusion:\u00a0E. coli\u00a0is the most common cause of UTI and ESBL production leads to increased resistance to common antibiotics and complicates treatment strategies.<\/jats:p>","DOI":"10.18502\/ijm.v17i1.17800","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T03:08:24Z","timestamp":1739243304000},"source":"Crossref","is-referenced-by-count":0,"title":["Incidence of extended spectrum beta-lactamase (ESBL) producing Escherichia coli isolated from women with urinary tract infections in Jordan"],"prefix":"10.18502","author":[{"given":"Rania Al-","family":"Groom","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2025,2,4]]},"container-title":["Iranian Journal of Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/17800\/16572","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/17800\/16572","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T11:21:37Z","timestamp":1772104897000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/17800"},"secondary":[{"URL":"https:\/\/ijm.tums.ac.ir\/index.php\/ijm\/article\/view\/5149","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,2,4]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijm.v17i1.17800","relation":{},"ISSN":["2008-4447","2008-3289"],"issn-type":[{"value":"2008-4447","type":"electronic"},{"value":"2008-3289","type":"print"}],"subject":[],"published":{"date-parts":[[2025,2,4]]}}}