{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:32:24Z","timestamp":1790879544702,"version":"4.1.0"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJM"],"abstract":"<jats:p>Background and Objectives: Scrub typhus, caused by Orientia tsutsugamushi, is a significant zoonotic illness in the Asia-Pacific region. Timely diagnosis is crucial, but overlapping symptoms and limitations of traditional diagnostic methods pose challenges. This study evaluates the diagnostic accuracy and utility of IgM ELISA, RT-PCR, and Rapid Card test for Scrub typhus, focusing on sensitivity, specificity, and practical applicability in endemic regions.\r\nMaterials and Methods: This cross-sectional study was conducted on 192 patients with suspected Scrub typhus at a tertiary care hospital from June to November 2024. Diagnostic tests included Rapid Card, IgM ELISA, and RT-PCR. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy were calculated, along with clin- ical and demographic data.\r\nResults: IgM ELISA had the highest sensitivity (96.30%) and specificity (100%), followed by Rapid Card (sensitivity: 93.55%, specificity: 99.38%) and RT-PCR (sensitivity: 92.86%, specificity: 99.44%). Common symptoms included fever (99.4%) and headache (95.8%). Positive cases were mostly males (56.7%-64.3%) and individuals aged 21-40 years.\r\nConclusion: IgM ELISA shows high sensitivity and specificity for Scrub typhus, while RT-PCR aids early detection. The Rapid Card offers a quick field alternative. Combining molecular and serological methods can enhance diagnostic accuracy.<\/jats:p>","DOI":"10.18502\/ijm.v17i4.19249","type":"journal-article","created":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T08:02:11Z","timestamp":1753862531000},"source":"Crossref","is-referenced-by-count":1,"title":["A comparative study on diagnostic accuracy of rapid card test, IgM ELISA and real time-PCR in detecting scrub typhus infection: a cross-sectional study from tertiary care hospital"],"prefix":"10.18502","author":[{"given":"Gopinath","family":"Ramalingam","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arundadhi","family":"Muthukumar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhanasezhian","family":"Aridass","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Krishnapriya","family":"Subramani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sucila Thangam","family":"Ganesan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7770","published-online":{"date-parts":[[2025,7,27]]},"container-title":["Iranian Journal of Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/19249\/17934","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/19249\/17934","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T11:19:36Z","timestamp":1772104776000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/19249"},"secondary":[{"URL":"https:\/\/ijm.tums.ac.ir\/index.php\/ijm\/article\/view\/5205","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,27]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijm.v17i4.19249","relation":{},"ISSN":["2008-4447","2008-3289"],"issn-type":[{"value":"2008-4447","type":"electronic"},{"value":"2008-3289","type":"print"}],"subject":[],"published":{"date-parts":[[2025,7,27]]}}}