{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T19:03:32Z","timestamp":1784228612995,"version":"3.55.0"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJM"],"abstract":"<jats:p>Background and Objectives: The antimicrobial resistance of Pseudomonas aeruginosa bacteria limits the spectrum of effective antibiotics. Considerable focus has been placed on the identification of more contemporary and cost-effective anti- microbial drugs. In this study, the antibacterial properties of a commonly used solvent, dimethyl sulfoxide (DMSO), against P. aeruginosa were investigated.\r\nMaterials and Methods: The microtiter broth dilution technique was employed to establish the minimum inhibitory concen- tration (MIC) and minimum bactericidal concentration (MBC) of DMSO. The solvent\u2019s impact on bacterial growth, biofilm formation and eradication was assessed. A quantitative polymerase chain reaction (qPCR) was carried out to assess the effect of varying DMSO concentrations ranging from 1% to 8% (v\/v) on quorum sensing gene expression.\r\nResults: All P. aeruginosa strains exhibited a DMSO MIC of 25% v\/v and MBC of 50% v\/v. DMSO caused significant growth inhibition and suppression of biofilm formation in all P. aeruginosa strains at sub-inhibitory concentrations, i.e. 1%-8% v\/v. At these concentrations, the samples showed a reduction in biomass and reduced metabolic activity. These effects were concentration-dependent. A DMSO strength of 8% v\/v was associated with a statistically significant downregulation of most of the quorum sensing genes; at a DMSO titer of 1% v\/v, this effect was modest with only a few genes being signifi- cantly affected.\r\nConclusion: DMSO is a potential therapeutic agent against P. aeruginosa as it has been demonstrated that it exhibits anti- microbial characteristics. Moreover, the impact of DMSO on bacterial growth and biofilm formation complicates its use as a solvent in biologic and clinical research\r\n\u00a0<\/jats:p>","DOI":"10.18502\/ijm.v17i6.20362","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T18:49:43Z","timestamp":1765565383000},"source":"Crossref","is-referenced-by-count":2,"title":["Investigation of the effect of dimethyl sulfoxide on growth and biofilm formation of Pseudomonas aeruginosa"],"prefix":"10.18502","author":[{"given":"Hafez","family":"Al-Momani","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Safaa","family":"Mashal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hadeel","family":"AlGhawrie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7770","published-online":{"date-parts":[[2025,12,12]]},"container-title":["Iranian Journal of Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/20362\/19034","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/20362\/19034","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T11:15:48Z","timestamp":1772104548000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/20362"},"secondary":[{"URL":"https:\/\/ijm.tums.ac.ir\/index.php\/ijm\/article\/view\/4510","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,12]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijm.v17i6.20362","relation":{},"ISSN":["2008-4447","2008-3289"],"issn-type":[{"value":"2008-4447","type":"electronic"},{"value":"2008-3289","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12,12]]}}}