{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T12:03:33Z","timestamp":1772107413505,"version":"3.50.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJM"],"abstract":"<jats:p>Background and Objectives: Antibiotic-resistant bacteria are a growing global health concern, particularly in developing regions. Sulfonamides, once widely used, now face increasing resistance. This study assessed the prevalence, resistance profiles, and virulences traites of sulfonamide-resistant strains in S\u00e9tif, Algeria (2021-2023).\r\nMaterials and Methods: A total of 215 clinical isolates were collected from patients aged 1 day to 96 years (mean 42.7). Most were community-acquired (77.2%), with urinary tract infections predominating (49.3% in women, 32.1% in men). Identification and susceptibility testing followed standard microbiological and Kirby\u2013Bauer methods. Virulence factors (bio- film, hemolysin, protease, lecithinase, and lipase) were examined. Molecular docking compared sulfamethoxazole and tri- methoprim binding to their enzymatic targets.\r\nResults: Escherichia coli was the most frequent isolate (47.9%), followed by Enterobacter spp. (11.6%). Biofilm formation was common (88.8%), with complete production in Klebsiella, Citrobacter, Providencia, and Acinetobacter. Hemolysis pat- terns were \u03b1 (30.7%), \u03b2 (27.9%), and none (41.4%). Enzymatic activity included protease (48.8%), lecithinase (22.8%), and lipase (9.8%). High resistance was observed to penicillins (87.9%), cephalosporins (63.7%), and fluoroquinolones (56.3%). Resistance was lower to imipenem (33.0%) and amikacin (14.4%). Docking showed weaker sulfamethoxazole binding to DHPS than trimethoprim to DHFR.\r\nConclusion: The high prevalence of multidrug-resistant bacteria, especially E. coli, combined with biofilm and enzyme production, underscores the urgent need for careful antibiotic stewardship in this region.\r\n\u00a0<\/jats:p>","DOI":"10.18502\/ijm.v17i6.20370","type":"journal-article","created":{"date-parts":[[2025,12,12]],"date-time":"2025-12-12T18:49:37Z","timestamp":1765565377000},"source":"Crossref","is-referenced-by-count":0,"title":["Sulfonamide resistance, virulence traits, and in-silico target interactions among clinical isolates in Setif, Algeria (2021\u20132023)"],"prefix":"10.18502","author":[{"given":"Anfal","family":"Kara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Imane","family":"Krache","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naouel","family":"Boussoualim","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roufaida","family":"Bourouche","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naouel","family":"Hamani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Malek Nour","family":"Kheloufi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yacine","family":"Benguerba","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2025,12,12]]},"container-title":["Iranian Journal of Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/20370\/19042","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/20370\/19042","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T11:14:50Z","timestamp":1772104490000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/20370"},"secondary":[{"URL":"https:\/\/ijm.tums.ac.ir\/index.php\/ijm\/article\/view\/5592","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,12,12]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijm.v17i6.20370","relation":{},"ISSN":["2008-4447","2008-3289"],"issn-type":[{"value":"2008-4447","type":"electronic"},{"value":"2008-3289","type":"print"}],"subject":[],"published":{"date-parts":[[2025,12,12]]}}}