{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T12:17:35Z","timestamp":1780489055178,"version":"3.54.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJM"],"abstract":"<jats:p>Background and Objectives: Fluoroquinolones represent a class of antibiotics commonly used to manage Pseudomo- nas aeruginosa infections. The appearance of fluoroquinolone resistance in P. aeruginosa represents a critical challenge to healthcare systems.\r\nMaterials and Methods: Between January and December 2024, 650 clinical specimens were collected from Al-Hussein Teaching Hospital and Al-Rumaitha Hospital in Al-Muthanna, Iraq. P. aeruginosa was identified by conventional biochem- ical assays and confirmed with the Vitek 2\u00ae system. Susceptibility testing was performed by disk diffusion. PCR was con- ducted to detect plasmid-mediated quinolone resistance determinants, namely qnrA, qnrB, qnrD, qnrS, aac(6\u2032)-Ib-cr, and qepA.\r\nResults: Among the 650 specimens analyzed, 374 (57.54%) were positive for bacterial culture, with P. aeruginosa represent- ing 40.10% (150\/374) of the identified isolates. Among these, 39 (26%) exhibited resistance to at least one fluoroquinolone used. The most frequently detected gene was qnrB (67.65%), followed by qnrD (61.76%), qnrS (55.88%), aac(6')-Ib-cr (47.00%), and qepA (41.17%), while qnrA and qnrC were not detected in any isolate.\r\nConclusion: The fluoroquinolone resistance and widespread occurrence in isolates of P. aeruginosa from Al-Muthanna Province pose a challenge to infection management, as mobile genetic elements facilitate the rapid dissemination of re- sistance and limit available therapeutic options, emphasizing the necessity for genetic monitoring and effective antibiotic management.\r\n\u00a0<\/jats:p>","DOI":"10.18502\/ijm.v18i2.21300","type":"journal-article","created":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T14:37:20Z","timestamp":1777127840000},"source":"Crossref","is-referenced-by-count":0,"title":["Molecular characterization of plasmid-mediated quinolone resistance genes in clinical isolates of Pseudomonas aeruginosa from Al-Muthanna Province, Iraq"],"prefix":"10.18502","author":[{"given":"Sarah A. Al-","family":"Khafaji","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Firas Srhan Abd","family":"Al-Mayahi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7770","published-online":{"date-parts":[[2026,4,25]]},"container-title":["Iranian Journal of Microbiology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/21300\/19736","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/download\/21300\/19736","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T11:48:34Z","timestamp":1780487314000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJM\/article\/view\/21300"},"secondary":[{"URL":"https:\/\/ijm.tums.ac.ir\/index.php\/ijm\/article\/view\/5802","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,4,25]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijm.v18i2.21300","relation":{},"ISSN":["2008-4447","2008-3289"],"issn-type":[{"value":"2008-4447","type":"electronic"},{"value":"2008-3289","type":"print"}],"subject":[],"published":{"date-parts":[[2026,4,25]]}}}