{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:20:41Z","timestamp":1787239241392,"version":"build-2736575974"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJPA"],"abstract":"<jats:p>Background: In the present study, we examined the effects of Fe3O4@bio-MOF nanoparticle (Nano-FO) plus artemisinin (Art) and glucantime (Glu) or shark cartilage extract (ShCE) on Leishmania major in vitro and in vivo.\u00a0\r\nMethods: This experimental study was conducted at the laboratory of Department of Parasitology, Tarbiat Modares University, Tehran, Iran during 2016-2017. The promastigote and amastigote assays were performed were conducted at the presence of 3.12- 400 \u00b5g\/mL of the drug combinations. According to in vitro IC50 results, the combinations of 12.5\u00b5g\/mL Nano-FO with 25 \u00b5g\/mL Art as well as 200 \u00b5g\/mL Glu and 0.5 mL of 20 mg\/kg of ShCE were used to treat BALB\/c mice. During and at the end of the treatment, the lesion sizes were measured. Parasite loads, cytokine levels were evaluated at the end of the treatment.\r\nResults: The IC50 of Fe3O4@bio-MOF-Artemisinin (Nano-FO\/Art), Fe3O4@bio-MOF-Glucantime (Nano-FO\/Glu), and Fe3O4@bio-MOF-Shark cartilage extract (Nano-FO\/ShCE) on promasitigotes were 12.58\u00b10.12, 235\u00b10.17, and 18.54\u00b10.15, respectively. These results on amastigotes were 10.32\u00b10.01, 187\u00b10.03, and 338\u00b10.07 \u00b5g\/mL, respectively. The apoptosis percentage of these combinations were 32.54%, 20.59%, and 15.68% in promastigotes and 15.68%, 12.84%, and 3.51% in infected macrophages, respectively with no toxicity on uninfected macrophages. In vivo results showed that the size of lesions significantly decreased against all drugs combinations, but Nano-FO\/Art combination with Selectivity Index of 23.62 value was safe, and more effective on healing of lesions than other drugs combinations (P=0.003).\r\nConclusion: This study suggested that Nano-FO\/Art combination can be considered as an anti-leishmania combination therapy in CL induced by L. major.<\/jats:p>","DOI":"10.18502\/ijpa.v15i4.4859","type":"journal-article","created":{"date-parts":[[2020,12,15]],"date-time":"2020-12-15T06:50:48Z","timestamp":1608015048000},"source":"Crossref","is-referenced-by-count":20,"title":["Fe3O4@Bio-MOF Nanoparticles Combined with Artemisinin, Glucantime\u00ae, or Shark Cartilage Extract on Iranian Strain of Leishmania major (MRHO\/IR\/75\/ER): An In-Vitro and In-Vivo Study"],"prefix":"10.18502","author":[{"given":"Fatemeh\u200c\u200c\u200c\u200c","family":"GHAFARIFAR","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soheila","family":"MOLAIE","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reza","family":"ABAZARI","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zoheir- Mohammad","family":"HASAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masoud","family":"FOROUTAN","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7770","published-online":{"date-parts":[[2020,12,15]]},"container-title":["Iranian Journal of Parasitology"],"original-title":[],"link":[{"URL":"https:\/\/18.184.16.47\/index.php\/IJPA\/article\/download\/4859\/4163","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/18.184.16.47\/index.php\/IJPA\/article\/download\/4859\/4163","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T11:15:35Z","timestamp":1734434135000},"score":1,"resource":{"primary":{"URL":"https:\/\/18.184.16.47\/index.php\/IJPA\/article\/view\/4859"},"secondary":[{"URL":"https:\/\/ijpa.tums.ac.ir\/index.php\/ijpa\/article\/view\/3179","label":"UNIVERSITY"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/view\/4859","label":"PUBLISHER"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,15]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijpa.v15i4.4859","relation":{},"ISSN":["1735-7020"],"issn-type":[{"value":"1735-7020","type":"print"}],"subject":[],"published":{"date-parts":[[2020,12,15]]}}}