{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T17:10:16Z","timestamp":1734455416495,"version":"3.30.2"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJPA"],"abstract":"<jats:p>Background: Leishmania is a mandatory intracellular pathogen and causing neglected disease. Hence, protection against leishmaniasis by a development vaccine is an important subject. This study aimed to design a poly-epitope vaccine for cutaneous leishmaniasis.\r\nMethods: The present study was conducted in the Parasitology Department of Tarbiat Modares University, Tehran, Iran during 2017-2019. Several bioinformatics methods at online servers were used for prediction of different aspects of polyepitope, including, physico-chemical attributes, allergenicity, antigenicity, secondary and tertiary structures, B-cell, T-cell and MHC (I, II) potential epitopes of LACK, LEIF, GP63 and SMT antigens of L. major.\r\nResults: After designing the construct (GLSL), the outputs of PTM sites demonstrated that the poly-epitope had 57 potential sites for phosphorylation. Furthermore, the secondary of GLSL structure includes 59.42%, 20.94% and 19.63% for random coil, extended strand and alpha-helix, respectively. The GLSL is an immunogenic protein with an acceptable antigenicity (0.8410) and non-allergen. Afterward, 20 potential epitopes of LACK, LEIF, GP63 and SMT antigens were linked by a flexible linker (SAPGTP), then was synthesized, and sub-cloned in pLEXY\u2013 neo2. The results were confirmed the expression of 38.7 kDa poly-epitope in secretory and cytosolic sites, separately.\r\nConclusion: A good expression in the L. tarentulae and confirmation of the GLSL poly-epitope could be a basis for developing a vaccine candidate against leishmaniasis that should be confirmed via experimental tests in BALB\/c mice.<\/jats:p>","DOI":"10.18502\/ijpa.v16i2.6267","type":"journal-article","created":{"date-parts":[[2021,6,8]],"date-time":"2021-06-08T07:38:42Z","timestamp":1623137922000},"source":"Crossref","is-referenced-by-count":1,"title":["A Novel Chimeric Antigen as a Vaccine Candidate against Leishmania major: In silico Analysis"],"prefix":"10.18502","author":[{"given":"Niloofar","family":"Bavarsad Ahmadpour","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdolhossein","family":"Dalimi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Majid","family":"Pirestani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Javid","family":"Sadraei","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2021,6,2]]},"container-title":["Iranian Journal of Parasitology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/download\/6267\/6049","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/download\/6267\/6049","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T16:31:41Z","timestamp":1734453101000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/view\/6267"},"secondary":[{"URL":"https:\/\/ijpa.tums.ac.ir\/index.php\/ijpa\/article\/view\/2816","label":"UNIVERSITY"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/view\/6267","label":"PUBLISHER"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,6,2]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijpa.v16i2.6267","relation":{},"ISSN":["1735-7020"],"issn-type":[{"type":"print","value":"1735-7020"}],"subject":[],"published":{"date-parts":[[2021,6,2]]}}}