{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T17:40:11Z","timestamp":1734457211589,"version":"3.30.2"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJPA"],"abstract":"<jats:p>Background: Visceral leishmaniasis (VL) is a lethal parasitic disease, transmitted by sand fly vectors. Immunomodulatory properties of sand fly saliva proteins and their protective effects against Leishmania infection in pre-exposed animals suggest that a combination of an antigenic salivary protein along with a Leishmania antigen can be considered for designing a vaccine against leishmaniasis\r\nMethods: Three different fusion forms of L. infantum hypothetical protein (LiHyV) in combination with Phlebotomus kandelakii salivary apyrase (PkanAp) were subjected to in-silico analyses. Major Histocompatibility Complex (MHC) class I and II epitopes in both humans and BALB\/c mice were predicted. Antigenicity, immunogenicity, epitope conservancy, toxicity, and population coverage were also evaluated.\r\nResults: Highly antigenic promiscuous epitopes consisting of truncated LiHyV (10-285) and full-length PkanAp (21-329) were identified in human and was named Model 1. This model contained 25 MHC-I and 141 MHC-II antigenic peptides which among them, MPANSDIRI and AQSLFDFSGLALDSN were fully conserved. LALDSNATV, RCSSALVSI, ALVSINVPL, SAVESGALF of MHC-I epitopes, and 28 MHC-II binding epitopes showed 60% conservancy among various clades. A population coverage with a rate of &gt;75% in the Iranian population and &gt;70% in the whole world was also identified.\r\nConclusion: Based on this in-silico approach, the predicted Model 1 could potentially be used as a vaccine candidate against VL.<\/jats:p>","DOI":"10.18502\/ijpa.v17i2.9530","type":"journal-article","created":{"date-parts":[[2022,6,6]],"date-time":"2022-06-06T10:06:16Z","timestamp":1654509976000},"source":"Crossref","is-referenced-by-count":2,"title":["Immunoinformatics Evaluation of a Fusion Protein Composed of Leishmania infantum LiHyV and Phlebotomus kandelakii  Apyrase as a Vaccine Candidate against Visceral Leishmaniasis"],"prefix":"10.18502","author":[{"given":"Shima","family":"Fayaz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fariborz","family":"Bahrami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pezhman","family":"Fard-Esfahani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Parviz","family":"Parvizi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Golnaz","family":"Bahramali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soheila","family":"Ajdary","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2022,6,1]]},"container-title":["Iranian Journal of Parasitology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/download\/9530\/8919","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/download\/9530\/8919","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T17:00:18Z","timestamp":1734454818000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/view\/9530"},"secondary":[{"URL":"https:\/\/ijpa.tums.ac.ir\/index.php\/ijpa\/article\/view\/3466","label":"UNIVERSITY"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/view\/9530","label":"PUBLISHER"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,6,1]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijpa.v17i2.9530","relation":{},"ISSN":["1735-7020"],"issn-type":[{"type":"print","value":"1735-7020"}],"subject":[],"published":{"date-parts":[[2022,6,1]]}}}