{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,28]],"date-time":"2025-11-28T12:33:48Z","timestamp":1764333228228,"version":"3.30.2"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJPA"],"abstract":"<jats:p>Background: Infections by Plasmodium falciparum, are becoming increasingly difficult to treat. Therefore, there is an urgent need for novel antimalarial agents\u2019 discovery against infection. In present study, we described a 2\u2019-O-Methyl gapmer phosphorothioate oligonucleotide antisense targeting translation initiation region of 3D7 strain RH5 gene.\r\nMethods: The study was conducted in Pasteur Institute of Iran in 2020. ODNs effects were measured by microscopic examination and real time RT-PCR. For microscopy, microplates were charged with 2\u2019-OMe ODNs at different dilutions. Unsynchronized parasites were added to a total of 0.4 ml (0.4% parasitemia, 5% red blood cells), and slides were prepared. Proportion of infected cells was measured by counting at least 500 red blood cells.\r\nResults: RH5 genes start codon regions selected as conserved region besed on alignment results. Gap-RH5-As which was complementary to sequence surrounding AUG RH5 start codon signi\ufb01cantly reduced parasite growth (&gt;90% at 50 nM) compared to sense sequence control (Gap-RH5-Se) (17%), (P&lt;0.001). RH5 transcripts were dramatically reduced after exposed to ODNs at a concentration of 5-500 nM for 48 h.\r\nConclusion: Gemnosis delivery of a chimeric gapmer PS-ODN with 2\u2019-OMe modifications at both sides had high antisense activity at low concentrations (10-100 nM) and shown a good efficiency to reach to target mRNA in human RBCs. Anti-parasite effect was correlated to reduction of target gene mRNA level. In addition, 2\u2019-OMe ODNs free delivery is an effective way and does not need any carrier molecules or particles.<\/jats:p>","DOI":"10.18502\/ijpa.v17i4.11280","type":"journal-article","created":{"date-parts":[[2022,12,11]],"date-time":"2022-12-11T11:47:15Z","timestamp":1670759235000},"source":"Crossref","is-referenced-by-count":2,"title":["Potential of RH5 Antisense on Plasmodium falciparum  Proliferation Abatement"],"prefix":"10.18502","author":[{"given":"Sepand","family":"Razavi Vakhshourpour","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Nateghpour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nader","family":"Shahrokhi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afsaneh","family":"Motevalli Haghi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Mohebali","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haleh","family":"Hanifian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2022,12,11]]},"container-title":["Iranian Journal of Parasitology"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/download\/11280\/10740","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/download\/11280\/10740","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T17:17:10Z","timestamp":1734455830000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/view\/11280"},"secondary":[{"URL":"https:\/\/ijpa.tums.ac.ir\/index.php\/ijpa\/article\/view\/3439","label":"UNIVERSITY"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJPA\/article\/view\/11280","label":"PUBLISHER"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,12,11]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijpa.v17i4.11280","relation":{},"ISSN":["1735-7020"],"issn-type":[{"type":"print","value":"1735-7020"}],"subject":[],"published":{"date-parts":[[2022,12,11]]}}}