{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,24]],"date-time":"2025-01-24T05:16:48Z","timestamp":1737695808971,"version":"3.33.0"},"reference-count":29,"publisher":"Knowledge E DMCC","issue":"1","content-domain":{"domain":["knepublishing.com"],"crossmark-restriction":true},"short-container-title":["IJRM"],"abstract":"<jats:p>Background: Reversible inhibition of sperm under guidance (\u201cRISUG\u00ae\u201d) is a promising intravasal male contraceptive.\r\nObjective: An exploratory study was conducted with a concept of non-invasive, transcervical, single-intervention and reversible contraception using RISUG\u00ae in females.\r\nMaterials and Methods: In this experimental study, 60 adult Wistar albino female rats weighing 150-155 g, 3-4 months old were divided into four groups: group I: sham-operated control; group II: tubal occlusion with RISUG for 90 days; group III: tubal occlusion with RISUG\u00ae for 90 days and reversal with dimethyl sulphoxide and group IV: tubal occlusion with RISUG\u00ae for 90 days and reversal with 5% NaHCO3. Animals were subjected to bilateral fallopian tube occlusion with RISUG\u00ae and reversal with DMSO and NaHCO3. The estrous cycle, fertility and histology of fallopian tube were evaluated.\r\nResults: Group I showed 100% fertility during all mating schedules. Animals of experimental groups indicated positive mating, but 0% fertility was evident following 30, 60, and 90 days of tubal occlusion. However, after reversal, fertility steadily increased to normalcy in groups III (50% at 45 days, 80% at 105 days, 100% at 150 and 195 days) and IV (70% at 45 and 105 days, 100% at 150 and 195 days) animals. Group II illustrated disorganized inner cell linings and eosinated RISUG\u00ae implant-filled lumen. Reversal groups (III and IV) revealed complete restoration of cellular histo-architecture. Regular estrous cycle was noticed in all experimental groups.\r\nConclusion: RISUG\u00ae is suitable for single intervention, intratubular, reversible contraception in female rats.\r\nKey words: Contraception, Fallopian tube, Tubal occlusion, RISUG\u00ae, Reversible.<\/jats:p>","DOI":"10.18502\/ijrm.v19i1.8179","type":"journal-article","created":{"date-parts":[[2021,1,26]],"date-time":"2021-01-26T05:34:50Z","timestamp":1611639290000},"page":"47-56","update-policy":"https:\/\/doi.org\/10.18502\/knecrossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Reversible inhibition of sperm under guidance as an intratubular and reversible contraception in female rats: An experimental study"],"prefix":"10.18502","volume":"19","author":[{"given":"Abdul","family":"Salam Ansari","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kiran","family":"Sevliya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayesha","family":"Badar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nirmal","family":"Kumar Lohiya","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2021,1,25]]},"reference":[{"key":"110263","doi-asserted-by":"crossref","unstructured":"Ceballos G, Ehrlich PR, Dirzo R. 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An Int J of Obstet Gynaecol 2017; 124: 1851\u20131857.","DOI":"10.1111\/1471-0528.14719"},{"key":"110267","unstructured":"Sharma RS, Rajanna A, Singh BK, Mathur AK, Mukherjee AK. Current Status of development of RISUG: An intravasal injectable male contraceptive. In: Sharma RS, Rajanna A, Rajalakshmi M (Eds.). Recent advances and challenges in Reproductive Health Research. New Delhi: Indian Council of Medical Research; 2008. pp: 135\u2013149."},{"key":"110268","doi-asserted-by":"crossref","unstructured":"Lohiya NK, Manivannan B, Mishra PK, Pathak N, Balasubramanian SPA. Intravasal contraception with styrene maleic anhydride and its non-invasive reversal in langur monkeys (Presbytis entellus entellus). Contraception 1998; 58: 119\u2013128.","DOI":"10.1016\/S0010-7824(98)00073-0"},{"key":"110269","doi-asserted-by":"crossref","unstructured":"Lohiya NK, Suthar R, Khandelwal A, Goyal S, Ansari AS, Manivannan B. Sperm characteristics and teratology in rats following vas occlusion with RISUG and its reversal. Int J Androl 2010; 33: e198\u2013e206.","DOI":"10.1111\/j.1365-2605.2009.00992.x"},{"key":"110270","unstructured":"Lohiya NK, Alam I, Hussain M, Khan SR, Ansari AS. RISUG: An intravasal injectable male contraceptive. Indian J Med Res 2014; 140 (Suppl.): 63\u201372."},{"key":"110271","doi-asserted-by":"crossref","unstructured":"Ansari AS, Alam I, Hussain M, Khan SR, Lohiya NK. Evaluation of genotoxicity in leukocytes and testis following intra-vasal contraception with RISUG and its reversal by DMSO and NaHCO3 in Wistar albino rats. Reprod Toxicol 2013; 36: 53\u201359.","DOI":"10.1016\/j.reprotox.2012.11.005"},{"key":"110272","doi-asserted-by":"crossref","unstructured":"Ansari AS, Hussain M, Khan SR, Lohiya NK. Relative suitability of DMSO and NaHCO3 for reversal of RISUG\u00ae induced long-term contraception. 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