{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,20]],"date-time":"2026-04-20T18:14:33Z","timestamp":1776708873096,"version":"3.51.2"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAD"],"abstract":"<jats:p>Background: Culex pipiens has a significant public health importance since it is an important vector of West Nile virus and Rift Valley fever virus. We, therefore, aimed to determine the in- secticide resistance level in Cx. pipiens populations in the Aegean and Mediterranean regions of Turkey.\r\nMethods: Bioassays have been carried out against Dichlorodiphenyltrichloroethane (DDT) (4%), Malathion (5%), Fenitrothion (1%), Propoxur (0.1%), Bendiocarb (0.1%), Permethrin (0.75%) and Deltamethrin (0.05%). Biochemical analyses have been performed to detect non-specific esterase, mixed function oxidase, glutathione-s-transferase and acetylcholinesterase levels. A knockdown resistance (kdr) (L1014F) and Acetylcholinesterase (Ace-1) (G119S) mutations have been detected by using allele-specific primers and a polymerase chain reaction (PCR) amplifica- tion of specific alleles (PASA) diagnostic test was performed for detection of F290V mutation.\r\nResults: Bioassay results showed that all Cx. pipiens populations were resistant to DDT, Mal- athion, Fenitrothion, Bendiocarb, Propoxur and some of the populations have started to gain Permethrin and Deltamethrin resistance. Biochemical analyses results revealed that altered glu- tathione-s-transferases, P450 monooxygenases, esterase levels might be responsible for DDT, or- ganophosphate, carbamate and pyrethroid resistance in Cx. pipiens populations. Results showed mild to high frequency of L1014F, low frequency of F290V but no Ace-1 G119S mutation within the populations. Additionally, acetylcholinesterase insensitivity was not significantly high within the most of these populations.\r\nConclusion: Overall results may help to fulfil the lacking information in the literature regarding insecticide resistance status and underlying mechanism of Culex pipiens populations of the Mediter- ranean and Aegean region of Turkey by using all bioassays, molecular tests and biochemical assays.\r\n\u00a0<\/jats:p>","DOI":"10.18502\/jad.v15i4.10505","type":"journal-article","created":{"date-parts":[[2022,9,4]],"date-time":"2022-09-04T06:34:44Z","timestamp":1662273284000},"source":"Crossref","is-referenced-by-count":3,"title":["Insecticide Resistance and Mechanisms of Culex pipiens Populations in the Mediterranean and Aegean Regions of Turkey During 2017\u20132018"],"prefix":"10.18502","author":[{"given":"Sare","family":"\u0130lknur Yava\u015fo\u011flu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fatih","family":"Mehmet \u015eim\u015fek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2022,9,3]]},"container-title":["Journal of Arthropod-Borne Diseases"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/download\/10505\/9894","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/download\/10505\/9894","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T17:08:15Z","timestamp":1734455295000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/view\/10505"},"secondary":[{"URL":"https:\/\/jad.tums.ac.ir\/index.php\/jad\/article\/view\/1299","label":"UNIVERSITY"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/view\/10505","label":"PUBLISHER"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,9,3]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/jad.v15i4.10505","relation":{},"ISSN":["2322-2271","1735-7179"],"issn-type":[{"value":"2322-2271","type":"electronic"},{"value":"1735-7179","type":"print"}],"subject":[],"published":{"date-parts":[[2022,9,3]]}}}