{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T13:56:39Z","timestamp":1772027799509,"version":"3.50.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAD"],"abstract":"<jats:p>Background: Cockroaches, recognized as mechanical vectors, play a crucial role in transmitting microbial pathogens. Staphylococcus aureus (S. aureus), particularly antibiotic-resistant strains, poses a significant threat as a nosocomial path\u00adogen. This study aimed to investigate the resistance profiles to gentamicin, vancomycin, and antiseptics in S. aureus strains isolated from cockroaches in hospitals affiliated with Babol University of Medical Sciences.\r\nMethods: In this cross-sectional study, 60 S. aureus strains were isolated from 376 cockroaches in three university-affiliated hospitals. Antibiotic susceptibility to gentamicin and vancomycin was tested by disk diffusion and agar dilu\u00adtion. PCR was used to detect resistance and antiseptic genes, and MLVA typing determined the genetic relatedness of resistant isolates.\r\nResults: Among the 60 bacterial isolates, 46.7% (28) displayed resistance to gentamicin. The frequencies of aminogly\u00adcoside resistance coding genes (AMEs) for the aac(6')-Ie+aph(2\u2033), ant(4')-Ia, aph(3')-IIIa and ant(6)-Ia genes were 64.3%, 42.8%, 17.8%, and 46.4%, respectively. Only 3.3% (2 isolates) exhibited vancomycin resistance, with one iso\u00adlate (1.7%) carrying the vanA gene. The frequencies of genes encoding the antiseptic resistance genes qacA\/B, qacC, qacD, psmA, sasX, and smr were 5%, 20%, 18.3%, 26.4%, 1.2%, and 31.7%, respectively. Analysis of agr gene types showed that agr type I was the most prevalent. In addition, the multiple-locus variable number tandem-repeat analysis (MLVA) identified 29 unique type sequences among the identified antibiotic-resistant isolates.\r\nConclusion: The high genetic diversity among antibiotic-resistant S. aureus isolates, as revealed by MLVA, under\u00adscores the importance of controlling hospital cockroach populations to curb the spread of antibiotic resistance.\r\n\u00a0<\/jats:p>","DOI":"10.18502\/jad.v19i1.19997","type":"journal-article","created":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T10:27:19Z","timestamp":1760956039000},"source":"Crossref","is-referenced-by-count":0,"title":["Antimicrobials Resistance Profiling and Clonal Lineages of Staphylococcus aureus Isolated from Cockroaches in University-Affiliated Hospitals, 2023"],"prefix":"10.18502","author":[{"given":"Mohsen","family":"Karami","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehdi","family":"Safari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasin","family":"Saberi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seyedeh Zohre","family":"Mirbagheri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mehrdad","family":"Halaji","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jalal","family":"Jafarzadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abazar","family":"Pournajaf","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2025,10,20]]},"container-title":["Journal of Arthropod-Borne Diseases"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/download\/19997\/18563","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/download\/19997\/18563","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T12:56:48Z","timestamp":1772024208000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/view\/19997"},"secondary":[{"URL":"https:\/\/jad.tums.ac.ir\/index.php\/jad\/article\/view\/1760","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10,20]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/jad.v19i1.19997","relation":{},"ISSN":["2322-2271","1735-7179"],"issn-type":[{"value":"2322-2271","type":"electronic"},{"value":"1735-7179","type":"print"}],"subject":[],"published":{"date-parts":[[2025,10,20]]}}}