{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T13:02:02Z","timestamp":1784466122345,"version":"3.55.0"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAD"],"abstract":"<jats:p>Background: Scorpion venom is a complex mixture containing toxic peptides, free amino acids, enzymes, nucleotides, lipids, amines, mucoproteins and other bioactive components. It has been reported to exhibit a range of medicinal prop\u00aderties, including anticancer, antithrombotic, anticoagulant, fibrinolytic, analgesic, antitumor and antiepileptic effects. This study aimed to evaluate the anticancer effects of crude venom from Odontobuthus doriae on the Michigan Cancer Foundation-7 (MCf-7) breast cancer cell line.\r\nMethods: 2\u00d7104 MCF-7 cancer cells were cultured in T25 flasks containing Dulbecco\u2019s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. After overnight incuba\u00adtion, the culture medium was replaced with different concentrations of crude venom (0.2, 0.48, 0.97, 1.95, 3.9, 7.81, 15.62, 31.25, 62.5, 125, 250, 500 \u03bcg\/mL). The cytotoxic effects were assessed using the MTT reduction assay at 24, 48 and 72 hours post-treatment, performed in triplicate. Absorbance was measured at 570 nm using an ELISA reader.\r\nResults: A concentration-dependent decrease in cell viability was observed. A statistically significant difference in cy\u00adtotoxicity was observed between the 24 hour and the 48\/72-hour treatments, while no significant difference was noted between the 48 and 72 hour time points. The IC\u2085\u2080 values were calculated to be 4.775 \u00b5g\/mL (24 h), 31.87 \u00b5g\/mL (48 h), and 3.543 \u00b5g\/mL (72 h).\r\nConclusion: The crude venom of O. doriae exhibits significant cytotoxic effects against MCF-7 breast cancer cells in a dose- and time-dependent manner, suggesting its potential as a natural anticancer agent.<\/jats:p>","DOI":"10.18502\/jad.v20i1.21968","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T11:36:00Z","timestamp":1782732960000},"source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Cytotoxic Effects of Odontobuthus doriae Crude Venom on the MCF-7 Breast Cancer Cell Line"],"prefix":"10.18502","author":[{"given":"Ghazal","family":"Tashakori","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasser","family":"Mohammadpour-Dounighi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir Ahmad","family":"Akhavan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hamid Reza","family":"Basseri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khosro","family":"Abdi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farzaneh","family":"Tofighi-Zavareh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sahar","family":"Khorasani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahboobe","family":"Fatemi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akbar","family":"Biglarian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Akbar","family":"Saboor-Yaraghi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Javad","family":"Rafinejad","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7770","published-online":{"date-parts":[[2026,6,29]]},"container-title":["Journal of Arthropod-Borne Diseases"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/download\/21968\/20316","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/download\/21968\/20316","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T12:10:33Z","timestamp":1784463033000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/view\/21968"},"secondary":[{"URL":"https:\/\/jad.tums.ac.ir\/index.php\/jad\/article\/view\/1886","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,29]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/jad.v20i1.21968","relation":{},"ISSN":["2322-2271","1735-7179"],"issn-type":[{"value":"2322-2271","type":"electronic"},{"value":"1735-7179","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6,29]]}}}