{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T13:02:03Z","timestamp":1784466123104,"version":"3.55.0"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JAD"],"abstract":"<jats:p>Background: Entomotoxicology provides an alternative matrix for detecting drugs in decomposed human remains when conventional specimens are unavailable. This study aimed to develop and validate a quantitative method for de\u00adtecting methamphetamine (MA) in different developmental stages of the blowfly Lucilia sericata.\r\nMethods: Immature stages of L. sericata (larvae, pupae, empty puparia) were reared on chicken liver spiked with MA at concentrations of 45, 90 and 180 ng\/mg. Samples underwent liquid\u2013liquid extraction, derivatization via acetylation, and analysis using gas chromatography-mass spectrometry (GC-MS). The method was validated according to interna\u00adtional guidelines, evaluating limit of detection (LOD), limit of quantification (LOQ), linearity, precision, accuracy and repeatability.\r\nResults: The method showed strong linearity across the studied concentration range (R\u00b2= 0.9817\u20130.9934). The limit of detection and LOQ were 15 ng\/mg and 45 ng\/mg, respectively, with a relative standard deviation (RSD)\u2264 20% at the LOQ level. Intra- and inter-day precision showed RSD values&lt; 10%, and accuracy ranged from 98.55% to 100.73%. Methamphetamine was detected in all immature stages and empty puparia. The highest observed concentrations were detected in third-instar larvae and pupae, whereas no drug residues were detected in adult flies. Methamphetamine re\u00adtention in empty puparia supports their potential forensic relevance for retrospective drug detection.\r\nConclusion: This validated method demonstrated acceptable sensitivity, precision, accuracy and repeatability for quan\u00adtitative entomotoxicological analysis. The detection of methamphetamine across insect developmental stages suggests the suitability of this approach as a supplementary tool for forensic investigations involving decomposed remains.\r\n\u00a0<\/jats:p>","DOI":"10.18502\/jad.v20i1.21969","type":"journal-article","created":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T11:35:58Z","timestamp":1782732958000},"source":"Crossref","is-referenced-by-count":0,"title":["Validation and Quantification of Methamphetamine in Empty Puparia of Lucilia sericata (Diptera: Calliphoridae) Using Liquid-Liquid Extraction and Gas Chromatography-Mass Spectrometry"],"prefix":"10.18502","author":[{"given":"Seyedeh Zahra","family":"Parkhideh","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Forouzesh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Akhgari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamran","family":"Akbarzadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ali Mohammad","family":"Ali Mohammadi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayena","family":"Rafizadeh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yavar","family":"Rassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7770","published-online":{"date-parts":[[2026,6,29]]},"container-title":["Journal of Arthropod-Borne Diseases"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/download\/21969\/20317","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/download\/21969\/20317","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T12:10:32Z","timestamp":1784463032000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JAD\/article\/view\/21969"},"secondary":[{"URL":"https:\/\/jad.tums.ac.ir\/index.php\/jad\/article\/view\/1926","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,29]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/jad.v20i1.21969","relation":{},"ISSN":["2322-2271","1735-7179"],"issn-type":[{"value":"2322-2271","type":"electronic"},{"value":"1735-7179","type":"print"}],"subject":[],"published":{"date-parts":[[2026,6,29]]}}}