{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T05:01:29Z","timestamp":1772773289098,"version":"3.50.1"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":["kne-publishing.com","knepublishing.com","jri.ir"],"crossmark-restriction":true},"short-container-title":["JRI"],"abstract":"<jats:p>Background: Although rescue intracytoplasmic sperm injection (r-ICSI) is extensively used worldwide, the indication of r-ICSI and its optimal timing remains obscure. This study aimed to assess the outcomes of r-ICSI following in vitro fertilization in different timings when fertilization is confirmed.\r\nMethods: This study included 5,156 cycles (47,785 eggs). Fertilization was confirmed by polar body analysis after 4 and 6 hr of coincubation of the sperm and oocyte. Oocytes that underwent IVF were divided into two groups based on the time when a second polar body was detected in more than 30% of all oocytes (Four-hr group and six-hr group). If the second polar body was not detected or was present in less than 30% of all oocytes after six hr of coincubation, rescue-ICSI (r-ICSI) was performed for oocytes without a second polar body (r-ICSI group).\r\nResults: The fertilization rates of two pronuclear (2PN) oocytes in the three groups (Four-hr group, six-hr group, and r-ICSI group) were 70.7%, 51.3%, and 58.0%, respectively. The blastocyst formation rates were 62.8%, 53.4%, and 42.9%, respectively.\r\nConclusion: Performing r-ICSI after six hr of coincubation can salvage cases with fertilization failure in IVF. The higher fertilization rate of r-ICSI indicates that all oocytes without signs of fertilization after six hr of coincubation should undergo r-ICSI.<\/jats:p>","DOI":"10.18502\/jri.v22i4.7650","type":"journal-article","created":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T04:08:50Z","timestamp":1635826130000},"update-policy":"https:\/\/doi.org\/10.18502\/knecrossmarkpolicy","source":"Crossref","is-referenced-by-count":3,"title":["Clinical Outcomes of Rescue Intracytoplasmic Sperm Injection at  Different Timings Following In Vitro Fertilization"],"prefix":"10.18502","author":[{"given":"Yuki","family":"Shiraiwa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noritoshi","family":"Enatsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuki","family":"Yamagami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koyu","family":"Furuhashi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshiroh","family":"Iwasaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junko","family":"Otsuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahide","family":"Shiotani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2021,11,1]]},"container-title":["Journal of Reproduction &amp; Infertility"],"original-title":[],"link":[{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JRI\/article\/download\/7650\/7337","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JRI\/article\/download\/7650\/7337","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T16:41:12Z","timestamp":1734453672000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JRI\/article\/view\/7650"},"secondary":[{"URL":"https:\/\/www.jri.ir\/article\/120137","label":"UNIVERSITY"},{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/JRI\/article\/view\/7650","label":"PUBLISHER"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,1]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/jri.v22i4.7650","relation":{},"ISSN":["2251-676X","2228-5482"],"issn-type":[{"value":"2251-676X","type":"electronic"},{"value":"2228-5482","type":"print"}],"subject":[],"published":{"date-parts":[[2021,11,1]]}}}