{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,12,18]],"date-time":"2024-12-18T05:36:30Z","timestamp":1734500190305,"version":"3.30.2"},"reference-count":0,"publisher":"Knowledge E DMCC","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["IJHOSCR"],"abstract":"<jats:p>Background: Arsenic three oxide (As2O3) is the treatment choice for acute promyelocytic leukemia (APL). Little is known about possible risk factors with\u00a0predictive\u00a0value for\u00a0toxicity\u00a0caused by As2O3. Biomethylation is considered to be a major pathway of detoxification for inorganic arsenics (iAs). Arsenic Methyltransferase\u00a0(AS3MT) is one of the key enzymes involved in the transfer of a methyl group from S-adenosyl-L-methionine to trivalent arsenical and plays a critical role in arsenic detoxification. Polymorphisms in hAS3MT lead to a change in the catalytic activity of the enzyme and may increase the risk of arsenic-related toxicity. In this study, we\u00a0investigated the association of\u00a0the AS3MT polymorphisms (rs11191439, rs3740390, and rs3740393) genes with hepatotoxicity in APL patients treated with As2O3.\r\nMaterials and Methods: Genotyping was performed in 140 adult patients\u00a0with APL treated\u00a0with As2O3 using PCR-RFLP for rs11191439 and tetra-primer\u00a0ARMS-PCR\u00a0for rs3740390 and rs3740393. The\u00a0results\u00a0of PCR-RFLP and ARMS-PCR were\u00a0confirmed\u00a0by\u00a0direct sequencing\u00a0of\u00a010 % of DNA samples. The results were analyzed using SNPStats, SPSS, and FinchTV. Hepatotoxicity was graded according to the\u00a0National Cancer Institute's Common Toxicity Criteria\u00a0(CTC).\r\nResults: Hepatotoxicity was seen in 52 of the 140 patients (37.1%), with grades I and II hepatotoxicity in 40 (28.6%) and grades III and IV hepatotoxicity in 12 (8.5%) patients.\u00a0 The association between the three polymorphisms and hepatotoxicity was evaluated using five genetic models and none of the three studied polymorphisms were significantly associated with hepatotoxicity.\r\nDiscussion: The results of\u00a0our study showed that AS3MT rs11191439, rs3740390, and rs3740393 polymorphisms are not associated with hepatotoxicity in APL patients. Genetic polymorphisms in enzymes which are involved in\u00a0arsenic metabolism have been shown to have ethnicity and race-related\u00a0differences. To more precisely characterize the association between AS3MT gene polymorphism and hepatotoxicity, future large-scale studies in non-Asian populations and other ethnicities are needed.<\/jats:p>","DOI":"10.18502\/ijhoscr.v17i4.13920","type":"journal-article","created":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T07:47:10Z","timestamp":1697960830000},"source":"Crossref","is-referenced-by-count":0,"title":["No Association of AS3MT Gene Polymorphisms with Susceptibility to Hepatotoxicity and in APL Patients Treated with AS2O3: A Single Center Study"],"prefix":"10.18502","author":[{"given":"Zeinab","family":"Joneidi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yousef","family":"Mortazavi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bahram","family":"Chahardouli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahrbano","family":"Rostami","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad","family":"Vaezi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Majid","family":"Nabipour","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alireza","family":"Biglari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ardeshir","family":"Ghavamzadeh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7770","published-online":{"date-parts":[[2023,10,20]]},"container-title":["International Journal of Hematology-Oncology and Stem Cell Research"],"original-title":[],"deposited":{"date-parts":[[2024,12,17]],"date-time":"2024-12-17T17:41:24Z","timestamp":1734457284000},"score":1,"resource":{"primary":{"URL":"https:\/\/publish.kne-publishing.com\/index.php\/IJHOSCR\/article\/view\/13920"},"secondary":[{"URL":"https:\/\/ijhoscr.tums.ac.ir\/index.php\/ijhoscr\/article\/view\/1656","label":"UNIVERSITY"}]},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,10,20]]},"references-count":0,"URL":"https:\/\/doi.org\/10.18502\/ijhoscr.v17i4.13920","relation":{},"ISSN":["2008-2207"],"issn-type":[{"type":"electronic","value":"2008-2207"}],"subject":[],"published":{"date-parts":[[2023,10,20]]}}}