{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T15:35:31Z","timestamp":1790523331331,"version":"4.1.0"},"reference-count":45,"publisher":"Impact Journals, LLC","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aging"],"published-print":{"date-parts":[[2020,1,12]]},"DOI":"10.18632\/aging.102632","type":"journal-article","created":{"date-parts":[[2020,1,12]],"date-time":"2020-01-12T15:38:42Z","timestamp":1578843522000},"page":"436-461","source":"Crossref","is-referenced-by-count":58,"title":["Aging exacerbates neutrophil pathogenicity in ischemic stroke"],"prefix":"10.18632","volume":"12","author":[{"given":"Meaghan A.","family":"Roy-O\u2019Reilly","sequence":"first","affiliation":[{"name":"Department of Neurology, University of Texas Health Science Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilda","family":"Ahnstedt","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Texas Health Science Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monica S.","family":"Spychala","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Texas Health Science Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yashasvee","family":"Munshi","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Texas Health Science Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaroslaw","family":"Aronowski","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Texas Health Science Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren H.","family":"Sansing","sequence":"additional","affiliation":[{"name":"Department of Neurology and Center for Neuroepidemiology and Clinical Neurological Research, Yale School of Medicine, New Haven, CT 06520, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise D.","family":"McCullough","sequence":"additional","affiliation":[{"name":"Department of Neurology, University of Texas Health Science Center, Houston, TX 77030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7892","published-online":{"date-parts":[[2020,1,12]]},"reference":[{"key":"1","doi-asserted-by":"publisher","first-page":"2145","DOI":"10.1016\/S0140-6736(15)61340-X","article-title":"Global, regional, and national disability-adjusted life years (DALYs) for 306 diseases and injuries and healthy life expectancy (HALE) for 188 countries, 1990-2013: quantifying the epidemiological transition.","volume":"38","author":"Ackerman","year":"2015","journal-title":"Lancet"},{"key":"2","doi-asserted-by":"publisher","first-page":"e67","DOI":"10.1161\/CIR.0000000000000558","article-title":"Heart Disease and Stroke Statistics-2018 Update: A Report From the American Heart Association.","volume":"13","author":"Fornage","year":"2018","journal-title":"Circulation"},{"key":"3","doi-asserted-by":"publisher","first-page":"869","DOI":"10.1016\/S1474-4422(16)00114-9","article-title":"Neuroprotection in acute stroke: targeting excitotoxicity, oxidative and nitrosative stress, and inflammation.","volume":"1","author":"Planas","year":"2016","journal-title":"Lancet Neurol"},{"key":"4","doi-asserted-by":"publisher","first-page":"661","DOI":"10.1007\/s13311-016-0483-x","article-title":"Inflammation and Stroke: an Overview.","volume":"1","author":"Iadecola","year":"2016","journal-title":"Neurotherapeutics"},{"key":"5","doi-asserted-by":"publisher","first-page":"888","DOI":"10.1038\/jcbfm.2015.45","article-title":"Targeting neutrophils in ischemic stroke: translational insights from experimental studies.","volume":"3","author":"Sharp","year":"2015","journal-title":"J Cereb Blood Flow Metab"},{"key":"6","doi-asserted-by":"publisher","first-page":"657","DOI":"10.1016\/j.immuni.2010.11.011","article-title":"Neutrophils, from marrow to microbes.","volume":"3","author":"Borregaard","year":"2010","journal-title":"Immunity"},{"key":"7","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1146\/annurev-pathol-020712-164023","article-title":"The multifaceted functions of neutrophils.","volume":"9","author":"Lowell","year":"2014","journal-title":"Annu Rev Pathol"},{"key":"8","doi-asserted-by":"publisher","first-page":"1311","DOI":"10.1001\/jamaneurol.2014.1238","article-title":"Translational research in acute central nervous system injury: lessons learned and the future.","volume":"7","author":"Wijdicks","year":"2014","journal-title":"JAMA Neurol"},{"key":"9","doi-asserted-by":"publisher","first-page":"3318","DOI":"10.1177\/0271678X17723783","article-title":"Is age a key factor contributing to the disparity between success of neuroprotective strategies in young animals and limited success in elderly stroke patients? Focus on protein homeostasis.","volume":"3","author":"Paschen","year":"2017","journal-title":"J Cereb Blood Flow Metab"},{"key":"10","doi-asserted-by":"publisher","first-page":"432","DOI":"10.1016\/j.nbd.2004.12.014","article-title":"Increased oxidative protein and DNA damage but decreased stress response in the aged brain following experimental stroke.","volume":"1","author":"Carmichael","year":"2005","journal-title":"Neurobiol Dis"},{"key":"11","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1177\/0271678X15608393","article-title":"O-linked \u03b2-N-acetylglucosamine modification of proteins is activated in post-ischemic brains of young but not aged mice: implications for impaired functional recovery from ischemic stress.","volume":"3","author":"Yang","year":"2016","journal-title":"J Cereb Blood Flow Metab"},{"key":"12","doi-asserted-by":"publisher","first-page":"3318","DOI":"10.4049\/jimmunol.1502021","article-title":"Age-Associated Resident Memory CD8 T Cells in the Central Nervous System Are Primed To Potentiate Inflammation after Ischemic Brain Injury.","volume":"19","author":"McCullough","year":"2016","journal-title":"J Immunol"},{"key":"13","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1016\/j.expneurol.2013.08.011","article-title":"Differential effects of aging and sex on stroke induced inflammation across the lifespan.","volume":"24","author":"McCullough","year":"2013","journal-title":"Exp Neurol"},{"key":"14","doi-asserted-by":"publisher","first-page":"551","DOI":"10.1086\/314873","article-title":"Elderly humans show prolonged in vivo inflammatory activity during pneumococcal infections.","volume":"18","author":"Pedersen","year":"1999","journal-title":"J Infect Dis"},{"key":"15","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1016\/j.exger.2013.12.010","article-title":"Age-related differences in the neutrophil response to pulmonary pseudomonas infection.","volume":"5","author":"Kovacs","year":"2014","journal-title":"Exp Gerontol"},{"key":"16","doi-asserted-by":"publisher","first-page":"160","DOI":"10.1159\/000360165","article-title":"Characterization of the inflammatory infiltrate in Streptococcus pneumoniae pneumonia in young and elderly patients.","volume":"8","author":"Tzankov","year":"2014","journal-title":"Pathobiology"},{"key":"17","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1007\/s00401-018-1859-2","article-title":"Aging alters the immunological response to ischemic stroke.","volume":"13","author":"Liu","year":"2018","journal-title":"Acta Neuropathol"},{"key":"18","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1016\/j.expneurol.2010.04.010","article-title":"Expression of Na-K-Cl cotransporter and edema formation are age dependent after ischemic stroke.","volume":"22","author":"McCullough","year":"2010","journal-title":"Exp Neurol"},{"key":"19","doi-asserted-by":"publisher","first-page":"157","DOI":"10.1007\/s11357-011-9214-8","article-title":"Age-related changes in AMP-activated protein kinase after stroke.","volume":"3","author":"McCullough","year":"2012","journal-title":"Age (Dordr)"},{"key":"20","doi-asserted-by":"publisher","first-page":"792","DOI":"10.1038\/jcbfm.2009.5","article-title":"Changes in experimental stroke outcome across the life span.","volume":"2","author":"McCullough","year":"2009","journal-title":"J Cereb Blood Flow Metab"},{"key":"21","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1016\/j.rinim.2015.12.001","article-title":"Ly6G-mediated depletion of neutrophils is dependent on macrophages.","volume":"6","author":"Spellberg","year":"2015","journal-title":"Results Immunol"},{"key":"22","doi-asserted-by":"publisher","first-page":"2905","DOI":"10.1128\/IAI.00356-12","article-title":"Neutrophils are essential for containment of Vibrio cholerae to the intestine during the proinflammatory phase of infection.","volume":"8","author":"Satchell","year":"2012","journal-title":"Infect Immun"},{"key":"23","doi-asserted-by":"publisher","first-page":"1489","DOI":"10.1182\/blood-2012-01-404046","article-title":"Ly6G ligation blocks recruitment of neutrophils via a \u03b22-integrin-dependent mechanism.","volume":"12","author":"Nigrovic","year":"2012","journal-title":"Blood"},{"key":"24","doi-asserted-by":"publisher","first-page":"138","DOI":"10.1016\/j.jneuroim.2018.04.015","article-title":"Implications of polymorphonuclear neutrophils for ischemic stroke and intracerebral hemorrhage: predictive value, pathophysiological consequences and utility as therapeutic target.","volume":"32","author":"Gunzer","year":"2018","journal-title":"J Neuroimmunol"},{"key":"25","doi-asserted-by":"publisher","first-page":"104","DOI":"10.1016\/j.expneurol.2017.08.016","article-title":"Genetic neutrophil deficiency ameliorates cerebral ischemia-reperfusion injury.","volume":"29","author":"Mortensen","year":"2017","journal-title":"Exp Neurol"},{"key":"26","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1136\/thoraxjnl-2014-205438","article-title":"Red, amber and green: the role of the lung in de-priming active systemic neutrophils.","volume":"6","author":"Stockley","year":"2014","journal-title":"Thorax"},{"key":"27","doi-asserted-by":"publisher","first-page":"1432","DOI":"10.1182\/blood-2005-03-0944","article-title":"Sequential activation of class IB and class IA PI3K is important for the primed respiratory burst of human but not murine neutrophils.","volume":"10","author":"Camps","year":"2005","journal-title":"Blood"},{"key":"28","doi-asserted-by":"publisher","first-page":"528","DOI":"10.1038\/nature15367","article-title":"Neutrophil ageing is regulated by the microbiome.","volume":"52","author":"Frenette","year":"2015","journal-title":"Nature"},{"key":"29","doi-asserted-by":"publisher","first-page":"459","DOI":"10.1146\/annurev-immunol-020711-074942","article-title":"Neutrophil function: from mechanisms to disease.","volume":"3","author":"Zychlinsky","year":"2012","journal-title":"Annu Rev Immunol"},{"key":"30","doi-asserted-by":"publisher","first-page":"4330","DOI":"10.1182\/blood.V88.11.4330.bloodjournal88114330","article-title":"Demonstration of reversible priming of human neutrophils using platelet-activating factor.","volume":"8","author":"Chilvers","year":"1996","journal-title":"Blood"},{"key":"31","doi-asserted-by":"publisher","first-page":"2327","DOI":"10.1182\/blood-2016-05-718999","article-title":"Aged neutrophils contribute to the first line of defense in the acute inflammatory response.","volume":"12","author":"Reichel","year":"2016","journal-title":"Blood"},{"key":"32","doi-asserted-by":"publisher","first-page":"3111","DOI":"10.1096\/fj.08-109876","article-title":"The role of the bone marrow in neutrophil clearance under homeostatic conditions in the mouse.","volume":"2","author":"Rankin","year":"2008","journal-title":"FASEB J"},{"key":"33","first-page":"919","article-title":"CD44 regulates phagocytosis of apoptotic neutrophil granulocytes, but not apoptotic lymphocytes, by human macrophages.","volume":"15","author":"Dransfield","year":"1997","journal-title":"J Immunol"},{"key":"34","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1186\/s12974-015-0314-8","article-title":"Aging increases microglial proliferation, delays cell migration, and decreases cortical neurogenesis after focal cerebral ischemia.","volume":"1","author":"Lizasoain","year":"2015","journal-title":"J Neuroinflammation"},{"key":"35","doi-asserted-by":"publisher","first-page":"H2954","DOI":"10.1152\/ajpheart.2000.279.6.H2954","article-title":"Interleukin-6 induces demargination of intravascular neutrophils and shortens their transit in marrow.","volume":"27","author":"Van Eeden","year":"2000","journal-title":"Am J Physiol Heart Circ Physiol"},{"key":"36","doi-asserted-by":"publisher","first-page":"1321","DOI":"10.1093\/rheumatology\/keu035","article-title":"Effects of IL-6 and IL-6 blockade on neutrophil function in vitro and in vivo.","volume":"5","author":"Moots","year":"2014","journal-title":"Rheumatology (Oxford)"},{"key":"37","doi-asserted-by":"publisher","first-page":"S4","DOI":"10.1093\/gerona\/glu057","article-title":"Chronic inflammation (inflammaging) and its potential contribution to age-associated diseases.","volume":"6","author":"Campisi","year":"2014","journal-title":"J Gerontol A Biol Sci Med Sci"},{"key":"38","first-page":"456","article-title":"The role of interleukin-8 in inflammation and mechanisms of regulation.","volume":"6","author":"Bickel","year":"1993","journal-title":"J Periodontol"},{"key":"39","doi-asserted-by":"publisher","first-page":"531","DOI":"10.1016\/j.ceca.2005.01.019","article-title":"Interleukin-8 primes oxidative burst in neutrophil-like HL-60 through changes in cytosolic calcium.","volume":"3","author":"Tschirhart","year":"2005","journal-title":"Cell Calcium"},{"key":"40","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.brainres.2007.06.043","article-title":"Age-related decrease of striatal neurogenesis is associated with apoptosis of neural precursors and newborn neurons in rat brain after ischemia.","volume":"116","author":"Sun","year":"2007","journal-title":"Brain Res"},{"key":"41","doi-asserted-by":"publisher","first-page":"753","DOI":"10.1016\/j.neurobiolaging.2006.12.007","article-title":"Early disruptions of the blood-brain barrier may contribute to exacerbated neuronal damage and prolonged functional recovery following stroke in aged rats.","volume":"2","author":"Rosen","year":"2008","journal-title":"Neurobiol Aging"},{"key":"42","doi-asserted-by":"publisher","first-page":"571","DOI":"10.1161\/STROKEAHA.113.002944","article-title":"Perfusion of ischemic brain in young and aged animals: a laser speckle flowmetry study.","volume":"4","author":"Liu","year":"2014","journal-title":"Stroke"},{"key":"43","doi-asserted-by":"publisher","first-page":"8701","DOI":"10.1523\/JNEUROSCI.23-25-08701.2003","article-title":"Aromatase cytochrome P450 and extragonadal estrogen play a role in ischemic neuroprotection.","volume":"2","author":"Hurn","year":"2003","journal-title":"J Neurosci"},{"key":"44","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.jneumeth.2008.12.028","article-title":"TTC, fluoro-Jade B and NeuN staining confirm evolving phases of infarction induced by middle cerebral artery occlusion.","volume":"17","author":"McCullough","year":"2009","journal-title":"J Neurosci Methods"},{"key":"45","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1016\/j.jneumeth.2004.04.029","article-title":"Infarct volume quantification in mouse focal cerebral ischemia: a comparison of triphenyltetrazolium chloride and cresyl violet staining techniques.","volume":"13","author":"Dempsey","year":"2004","journal-title":"J Neurosci Methods"}],"container-title":["Aging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.aging-us.com\/lookup\/doi\/10.18632\/aging.102632","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T15:46:02Z","timestamp":1599061562000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.aging-us.com\/lookup\/doi\/10.18632\/aging.102632"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,12]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,1,15]]}},"alternative-id":["102632","31927534"],"URL":"https:\/\/doi.org\/10.18632\/aging.102632","relation":{},"ISSN":["1945-4589"],"issn-type":[{"value":"1945-4589","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,12]]}}}