{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:30:59Z","timestamp":1786581059848,"version":"build-2736575974"},"reference-count":66,"publisher":"Impact Journals, LLC","issue":"1","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Aging"],"published-print":{"date-parts":[[2026,2,11]]},"DOI":"10.18632\/aging.206408","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:24:23Z","timestamp":1786580663000},"page":"1004-1027","source":"Crossref","is-referenced-by-count":0,"title":["<i>Indy<\/i>\n                    reduction decreases aging-related dysbiosis in\n                    <i>Drosophila<\/i>"],"prefix":"10.18632","volume":"18","author":[{"given":"Danielle N. A.","family":"Lesperance","sequence":"first","affiliation":[{"name":"Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shivani","family":"Padhi","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacob","family":"Macro","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genome Sciences, School of Medicine, University of Connecticut Health, Farmington, CT 06030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Olson","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genome Sciences, School of Medicine, University of Connecticut Health, Farmington, CT 06030, USA"},{"name":"Institute for Systems Genomics, School of Medicine, University of Connecticut Health, Farmington, CT 06030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ella","family":"Stanwood","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genome Sciences, School of Medicine, University of Connecticut Health, Farmington, CT 06030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kavitha","family":"Kannan","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genome Sciences, School of Medicine, University of Connecticut Health, Farmington, CT 06030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brenton","family":"Graveley","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genome Sciences, School of Medicine, University of Connecticut Health, Farmington, CT 06030, USA"},{"name":"Institute for Systems Genomics, School of Medicine, University of Connecticut Health, Farmington, CT 06030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Blanka","family":"Rogina","sequence":"additional","affiliation":[{"name":"Department of Genetics and Genome Sciences, School of Medicine, University of Connecticut Health, Farmington, CT 06030, USA"},{"name":"Institute for Systems Genomics, School of Medicine, University of Connecticut Health, Farmington, CT 06030, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nichole A.","family":"Broderick","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT 06269, USA"},{"name":"Department of Biology, Johns Hopkins University, Baltimore, MD 21218, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7892","published-online":{"date-parts":[[2026,8,13]]},"reference":[{"key":"1","doi-asserted-by":"publisher","first-page":"504","DOI":"10.1038\/nature08980","article-title":"The genetics of ageing.","volume":"46","author":"Kenyon","year":"2010","journal-title":"Nature"},{"key":"2","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1007\/978-3-540-48003-7_4","volume-title":"The Molecular Genetics of Aging (Internet)","author":"Helfand","year":"2000"},{"key":"3","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1002\/bies.10225","article-title":"Molecular genetics of aging in the fly: is this the end of the beginning?","volume":"2","author":"Helfand","year":"2003","journal-title":"Bioessays"},{"key":"4","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1146\/annurev-animal-022114-110829","article-title":"Animal models of aging research: implications for human aging and age-related diseases.","volume":"3","author":"Mitchell","year":"2015","journal-title":"Annu Rev Anim Biosci"},{"key":"5","doi-asserted-by":"publisher","first-page":"317","DOI":"10.1016\/j.exger.2010.09.001","article-title":"For the special issue: aging studies in Drosophila melanogaster.","volume":"4","author":"Rogina","year":"2011","journal-title":"Exp Gerontol"},{"key":"6","doi-asserted-by":"publisher","first-page":"203","DOI":"10.1146\/annurev-physiol-021119-034359","article-title":"Intestinal Stem Cell Aging: Origins and Interventions.","volume":"8","author":"Jasper","year":"2020","journal-title":"Annu Rev Physiol"},{"key":"7","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1016\/j.chom.2016.01.008","article-title":"Preventing Age-Related Decline of Gut Compartmentalization Limits Microbiota Dysbiosis and Extends Lifespan.","volume":"1","author":"Li","year":"2016","journal-title":"Cell Host Microbe"},{"key":"8","doi-asserted-by":"publisher","first-page":"e01117","DOI":"10.1128\/mBio.01117-14","article-title":"Microbiota-induced changes in drosophila melanogaster host gene expression and gut morphology.","volume":"5","author":"Broderick","year":"2014","journal-title":"mBio"},{"key":"9","doi-asserted-by":"publisher","first-page":"20150295","DOI":"10.1098\/rstb.2015.0295","article-title":"Friend, foe or food? Recognition and the role of antimicrobial peptides in gut immunity and Drosophila-microbe interactions.","volume":"37","author":"Broderick","year":"2016","journal-title":"Philos Trans R Soc Lond B Biol Sci"},{"key":"10","doi-asserted-by":"publisher","first-page":"1656","DOI":"10.1016\/j.celrep.2015.08.004","article-title":"Distinct Shifts in Microbiota Composition during Drosophila Aging Impair Intestinal Function and Drive Mortality.","volume":"1","author":"Clark","year":"2015","journal-title":"Cell Rep"},{"key":"11","doi-asserted-by":"publisher","first-page":"2137","DOI":"10.1126\/science.290.5499.2137","article-title":"Extended life-span conferred by cotransporter gene mutations in Drosophila.","volume":"29","author":"Rogina","year":"2000","journal-title":"Science"},{"key":"12","doi-asserted-by":"publisher","first-page":"14315","DOI":"10.1073\/pnas.222531899","article-title":"Functional characterization and immunolocalization of the transporter encoded by the life-extending gene Indy.","volume":"9","author":"Knauf","year":"2002","journal-title":"Proc Natl Acad Sci USA"},{"key":"13","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1042\/BJ20060409","article-title":"The life-extending gene Indy encodes an exchanger for Krebs-cycle intermediates.","volume":"39","author":"Knauf","year":"2006","journal-title":"Biochem J"},{"key":"14","doi-asserted-by":"publisher","first-page":"G402","DOI":"10.1152\/ajpgi.00371.2006","article-title":"Expression and functional features of NaCT, a sodium-coupled citrate transporter, in human and rat livers and cell lines.","volume":"29","author":"Gopal","year":"2007","journal-title":"Am J Physiol Gastrointest Liver Physiol"},{"key":"15","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1016\/j.cmet.2011.06.009","article-title":"Deletion of the mammalian INDY homolog mimics aspects of dietary restriction and protects against adiposity and insulin resistance in mice.","volume":"1","author":"Birkenfeld","year":"2011","journal-title":"Cell Metab"},{"key":"16","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1042\/BJ20031807","article-title":"Relevance of NAC-2, an Na+-coupled citrate transporter, to life span, body size and fat content in Caenorhabditis elegans.","volume":"37","author":"Fei","year":"2004","journal-title":"Biochem J"},{"key":"17","doi-asserted-by":"publisher","first-page":"782162","DOI":"10.3389\/fragi.2021.782162","article-title":"INDY-From Flies to Worms, Mice, Rats, Non-Human Primates, and Humans.","volume":"2","author":"Mishra","year":"2021","journal-title":"Front Aging"},{"key":"18","doi-asserted-by":"publisher","first-page":"1086","DOI":"10.18632\/aging.100854","article-title":"Prevention of diet-induced hepatic steatosis and hepatic insulin resistance by second generation antisense oligonucleotides targeted to the longevity gene mIndy (Slc13a5).","volume":"7","author":"Pesta","year":"2015","journal-title":"Aging (Albany NY)"},{"key":"19","doi-asserted-by":"publisher","first-page":"2277","DOI":"10.1073\/pnas.0812484106","article-title":"Long-lived Indy induces reduced mitochondrial reactive oxygen species production and oxidative damage.","volume":"10","author":"Neretti","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"20","doi-asserted-by":"publisher","first-page":"9262","DOI":"10.1073\/pnas.0904115106","article-title":"Long-lived Indy and calorie restriction interact to extend life span.","volume":"10","author":"Wang","year":"2009","journal-title":"Proc Natl Acad Sci USA"},{"key":"21","doi-asserted-by":"publisher","first-page":"553","DOI":"10.18632\/aging.100791","article-title":"Knockdown of Indy\/CeNac2 extends Caenorhabditis elegans life span by inducing AMPK\/aak-2.","volume":"7","author":"Schwarz","year":"2015","journal-title":"Aging (Albany NY)"},{"key":"22","doi-asserted-by":"publisher","first-page":"204","DOI":"10.3389\/fgene.2015.00204","article-title":"The role of INDY in metabolism, health and longevity.","volume":"6","author":"Rogers","year":"2015","journal-title":"Front Genet"},{"key":"23","doi-asserted-by":"publisher","first-page":"335","DOI":"10.18632\/aging.100658","article-title":"Increased mitochondrial biogenesis preserves intestinal stem cell homeostasis and contributes to longevity in Indy mutant flies.","volume":"6","author":"Rogers","year":"2014","journal-title":"Aging (Albany NY)"},{"key":"24","doi-asserted-by":"publisher","first-page":"705","DOI":"10.3390\/metabo11100705","article-title":"The Role of Citrate Transporter INDY in Metabolism and Stem Cell Homeostasis.","volume":"1","author":"Kannan","year":"2021","journal-title":"Metabolites"},{"key":"25","doi-asserted-by":"publisher","first-page":"66","DOI":"10.3389\/fgene.2017.00066","article-title":"INDY-A New Link to Metabolic Regulation in Animals and Humans.","volume":"8","author":"Rogina","year":"2017","journal-title":"Front Genet"},{"key":"26","doi-asserted-by":"publisher","first-page":"47","DOI":"10.3389\/fgene.2013.00047","article-title":"Indy mutations and Drosophila longevity.","volume":"4","author":"Rogina","year":"2013","journal-title":"Front Genet"},{"key":"27","doi-asserted-by":"publisher","first-page":"6136","DOI":"10.1074\/jbc.M208763200","article-title":"Structural and functional characteristics of two sodium-coupled dicarboxylate transporters (ceNaDC1 and ceNaDC2) from Caenorhabditis elegans and their relevance to life span.","volume":"27","author":"Fei","year":"2003","journal-title":"J Biol Chem"},{"key":"28","doi-asserted-by":"publisher","first-page":"3369","DOI":"10.1073\/pnas.0634985100","article-title":"Conditional tradeoffs between aging and organismal performance of Indy long-lived mutant flies.","volume":"10","author":"Marden","year":"2003","journal-title":"Proc Natl Acad Sci USA"},{"key":"29","doi-asserted-by":"publisher","first-page":"13","DOI":"10.3389\/fgene.2012.00013","article-title":"Indy mutants: live long and prosper.","volume":"3","author":"Frankel","year":"2012","journal-title":"Front Genet"},{"key":"30","doi-asserted-by":"publisher","first-page":"1455","DOI":"10.1016\/j.cell.2022.04.002","article-title":"Nutrition, longevity and disease: From molecular mechanisms to interventions.","volume":"18","author":"Longo","year":"2022","journal-title":"Cell"},{"key":"31","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1111\/j.1474-9726.2005.00181.x","article-title":"Behavioral, physical, and demographic changes in Drosophila populations through dietary restriction.","volume":"4","author":"Bross","year":"2005","journal-title":"Aging Cell"},{"key":"32","doi-asserted-by":"publisher","first-page":"e2311019120","DOI":"10.1073\/pnas.2311019120","article-title":"Late-life shift in caloric intake affects fly metabolism and longevity.","volume":"12","author":"Li","year":"2023","journal-title":"Proc Natl Acad Sci USA"},{"key":"33","doi-asserted-by":"publisher","first-page":"4017","DOI":"10.1007\/s11357-024-01233-w","article-title":"Extended lifespan in female Drosophila melanogaster through late-life calorie restriction.","volume":"4","author":"Li","year":"2024","journal-title":"Geroscience"},{"key":"34","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1186\/s40168-022-01249-4","article-title":"Effects of caloric restriction on the gut microbiome are linked with immune senescence.","volume":"1","author":"Sbierski-Kind","year":"2022","journal-title":"Microbiome"},{"key":"35","doi-asserted-by":"publisher","first-page":"634","DOI":"10.1007\/s11684-018-0670-8","article-title":"Calorie restriction and its impact on gut microbial composition and global metabolism.","volume":"1","author":"Zheng","year":"2018","journal-title":"Front Med"},{"key":"36","doi-asserted-by":"publisher","first-page":"96","DOI":"10.1038\/s42255-022-00716-4","article-title":"The antitumour effects of caloric restriction are mediated by the gut microbiome.","volume":"5","author":"Mao","year":"2023","journal-title":"Nat Metab"},{"key":"37","doi-asserted-by":"publisher","first-page":"272","DOI":"10.1038\/s41586-021-03663-4","article-title":"Caloric restriction disrupts the microbiota and colonization resistance.","volume":"59","author":"von Schwartzenberg","year":"2021","journal-title":"Nature"},{"key":"38","doi-asserted-by":"publisher","first-page":"4155","DOI":"10.1038\/s41467-024-48355-5","article-title":"Gut microbiome remodeling and metabolomic profile improves in response to protein pacing with intermittent fasting versus continuous caloric restriction.","volume":"1","author":"Mohr","year":"2024","journal-title":"Nat Commun"},{"key":"39","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/j.cmet.2010.11.010","article-title":"Metabolic regulation of stem cell behavior and implications for aging.","volume":"1","author":"Jasper","year":"2010","journal-title":"Cell Metab"},{"key":"40","doi-asserted-by":"publisher","first-page":"2333","DOI":"10.1101\/gad.1827009","article-title":"Invasive and indigenous microbiota impact intestinal stem cell activity through multiple pathways in Drosophila.","volume":"2","author":"Buchon","year":"2009","journal-title":"Genes Dev"},{"key":"41","doi-asserted-by":"publisher","first-page":"200","DOI":"10.1016\/j.chom.2009.01.003","article-title":"Drosophila intestinal response to bacterial infection: activation of host defense and stem cell proliferation.","volume":"5","author":"Buchon","year":"2009","journal-title":"Cell Host Microbe"},{"key":"42","doi-asserted-by":"publisher","first-page":"dev167643","DOI":"10.1242\/dev.167643","article-title":"JAK\/STAT signaling in stem cells and regeneration: from Drosophila to vertebrates.","volume":"14","author":"Herrera","year":"2019","journal-title":"Development"},{"key":"43","doi-asserted-by":"publisher","first-page":"93","DOI":"10.1007\/s00018-017-2671-1","article-title":"Role of gut microbiota in aging-related health decline: insights from invertebrate models.","volume":"7","author":"Clark","year":"2018","journal-title":"Cell Mol Life Sci"},{"key":"44","doi-asserted-by":"publisher","first-page":"56","DOI":"10.1038\/s41580-021-00411-4","article-title":"Molecular mechanisms of dietary restriction promoting health and longevity.","volume":"2","author":"Green","year":"2022","journal-title":"Nat Rev Mol Cell Biol"},{"key":"45","doi-asserted-by":"publisher","first-page":"5944","DOI":"10.1242\/jcs.113100","article-title":"Autocrine and paracrine unpaired signaling regulate intestinal stem cell maintenance and division.","volume":"12","author":"Osman","year":"2012","journal-title":"J Cell Sci"},{"key":"46","doi-asserted-by":"publisher","first-page":"616","DOI":"10.1002\/hep.29089","article-title":"The human longevity gene homolog INDY and interleukin-6 interact in hepatic lipid metabolism.","volume":"6","author":"von Loeffelholz","year":"2017","journal-title":"Hepatology"},{"key":"47","doi-asserted-by":"publisher","first-page":"108834","DOI":"10.1016\/j.jnutbio.2021.108834","article-title":"Dietary citrate acutely induces insulin resistance and markers of liver inflammation in mice.","volume":"9","author":"Branco","year":"2021","journal-title":"J Nutr Biochem"},{"key":"48","doi-asserted-by":"publisher","first-page":"e01369","DOI":"10.1128\/mSystems.01369-20","article-title":"Predicted Metabolic Function of the Gut Microbiota of Drosophila melanogaster.","volume":"6","author":"Ankrah","year":"2021","journal-title":"mSystems"},{"key":"49","doi-asserted-by":"publisher","first-page":"1305","DOI":"10.1242\/bio.017673","article-title":"Comparative evaluation of the genomes of three common Drosophila-associated bacteria.","volume":"5","author":"Petkau","year":"2016","journal-title":"Biol Open"},{"key":"50","doi-asserted-by":"publisher","first-page":"4236","DOI":"10.1038\/s41467-020-18049-9","article-title":"Metabolic cross-feeding in imbalanced diets allows gut microbes to improve reproduction and alter host behaviour.","volume":"1","author":"Henriques","year":"2020","journal-title":"Nat Commun"},{"key":"51","doi-asserted-by":"publisher","first-page":"E11951","DOI":"10.1073\/pnas.1809349115","article-title":"Microbiome interactions shape host fitness.","volume":"11","author":"Gould","year":"2018","journal-title":"Proc Natl Acad Sci USA"},{"key":"52","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1016\/s0899-9007(02)00782-7","article-title":"Antibacterial activity of citrate and acetate.","volume":"1","author":"Lee","year":"2002","journal-title":"Nutrition"},{"key":"53","doi-asserted-by":"publisher","first-page":"23","DOI":"10.3389\/fbioe.2014.00023","article-title":"Study on the Antimicrobial Properties of Citrate-Based Biodegradable Polymers.","volume":"2","author":"Su","year":"2014","journal-title":"Front Bioeng Biotechnol"},{"key":"54","doi-asserted-by":"publisher","first-page":"109942","DOI":"10.1016\/j.jnutbio.2025.109942","article-title":"Enhanced citrate consumption increases colon permeability in C57BL\/6J mice by affecting gut microbiota and activating HIF-1\u03b1.","volume":"14","author":"Costa-Bartuli","year":"2025","journal-title":"J Nutr Biochem"},{"key":"55","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1016\/j.cmet.2011.09.013","article-title":"Modulation of longevity and tissue homeostasis by the Drosophila PGC-1 homolog.","volume":"1","author":"Rera","year":"2011","journal-title":"Cell Metab"},{"key":"56","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/j.stem.2010.12.006","article-title":"Redox regulation by Keap1 and Nrf2 controls intestinal stem cell proliferation in Drosophila.","volume":"8","author":"Hochmuth","year":"2011","journal-title":"Cell Stem Cell"},{"key":"57","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1016\/j.exger.2010.11.003","article-title":"Regulation of Drosophila lifespan by JNK signaling.","volume":"4","author":"Biteau","year":"2011","journal-title":"Exp Gerontol"},{"key":"58","doi-asserted-by":"publisher","first-page":"975","DOI":"10.1038\/s41467-018-03070-w","article-title":"Early-life exposure to low-dose oxidants can increase longevity via microbiome remodelling in Drosophila.","volume":"9","author":"Obata","year":"2018","journal-title":"Nat Commun"},{"key":"59","doi-asserted-by":"publisher","first-page":"eaaw3159","DOI":"10.1126\/scisignal.aaw3159","article-title":"Host mitochondria influence gut microbiome diversity: A role for ROS.","volume":"1","author":"Yardeni","year":"2019","journal-title":"Sci Signal"},{"key":"60","doi-asserted-by":"publisher","first-page":"12974","DOI":"10.1073\/pnas.0405207101","article-title":"Drosophila lifespan enhancement by exogenous bacteria.","volume":"10","author":"Brummel","year":"2004","journal-title":"Proc Natl Acad Sci USA"},{"key":"61","doi-asserted-by":"publisher","first-page":"pyw020","DOI":"10.1093\/ijnp\/pyw020","article-title":"Growing up in a Bubble: Using Germ-Free Animals to Assess the Influence of the Gut Microbiota on Brain and Behavior.","volume":"1","author":"Luczynski","year":"2016","journal-title":"Int J Neuropsychopharmacol"},{"key":"62","doi-asserted-by":"publisher","first-page":"655","DOI":"10.1093\/genetics\/131.3.655","article-title":"latheo, a new gene involved in associative learning and memory in Drosophila melanogaster, identified from P element mutagenesis.","volume":"13","author":"Boynton","year":"1992","journal-title":"Genetics"},{"key":"63","doi-asserted-by":"publisher","first-page":"e01401","DOI":"10.1128\/AEM.01401-20","article-title":"Gut Bacteria Mediate Nutrient Availability in Drosophila Diets.","volume":"8","author":"Lesperance","year":"2020","journal-title":"Appl Environ Microbiol"},{"key":"64","doi-asserted-by":"publisher","first-page":"54219","DOI":"10.3791\/54219","article-title":"Rearing the Fruit Fly Drosophila melanogaster Under Axenic and Gnotobiotic Conditions.","volume":"11","author":"Koyle","year":"2016","journal-title":"J Vis Exp"},{"key":"65","doi-asserted-by":"publisher","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2.","volume":"1","author":"Love","year":"2014","journal-title":"Genome Biol"},{"key":"66","doi-asserted-by":"publisher","first-page":"5112","DOI":"10.1128\/AEM.01043-13","article-title":"Development of a dual-index sequencing strategy and curation pipeline for analyzing amplicon sequence data on the MiSeq Illumina sequencing platform.","volume":"7","author":"Kozich","year":"2013","journal-title":"Appl Environ Microbiol"}],"container-title":["Aging"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.aging-us.com\/lookup\/doi\/10.18632\/aging.206408","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T00:24:24Z","timestamp":1786580664000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.aging-us.com\/lookup\/doi\/10.18632\/aging.206408"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,11]]},"references-count":66,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2026,2,11]]}},"alternative-id":["206408"],"URL":"https:\/\/doi.org\/10.18632\/aging.206408","relation":{},"ISSN":["1945-4589"],"issn-type":[{"value":"1945-4589","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,11]]}}}