{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T07:05:43Z","timestamp":1781766343412,"version":"3.54.5"},"reference-count":41,"publisher":"Impact Journals, LLC","issue":"60","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncotarget"],"published-print":{"date-parts":[[2017,11,24]]},"DOI":"10.18632\/oncotarget.21093","type":"journal-article","created":{"date-parts":[[2017,9,20]],"date-time":"2017-09-20T19:01:11Z","timestamp":1505934071000},"page":"101333-101344","source":"Crossref","is-referenced-by-count":19,"title":["LC-MS-based metabolomics revealed SLC25A22 as an essential regulator of aspartate-derived amino acids and polyamines in <i>KRAS<\/i>-mutant colorectal cancer"],"prefix":"10.18632","volume":"8","author":[{"given":"Xiaona","family":"Li","sequence":"first","affiliation":[{"name":"State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arthur C.K.","family":"Chung","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shangfu","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lilan","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China"},{"name":"Guangdong Provincial Academy of Chinese Medical Sciences, The Second Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaying","family":"Xu","sequence":"additional","affiliation":[{"name":"Institute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, Hong Kong, China"},{"name":"Shenzhen Research Institute, The Chinese University of Hong Kong, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chichun","family":"Wong","sequence":"additional","affiliation":[{"name":"Institute of Digestive Disease and Department of Medicine and Therapeutics, State Key Laboratory of Digestive Disease, Li Ka Shing Institute of Health Sciences, Chinese University of Hong Kong, Hong Kong, China"},{"name":"Shenzhen Research Institute, The Chinese University of Hong Kong, Guangdong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongwei","family":"Cai","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, Hong Kong, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7892","published-online":{"date-parts":[[2017,9,20]]},"reference":[{"key":"1","doi-asserted-by":"publisher","first-page":"E359","DOI":"10.1002\/ijc.29210","article-title":"Cancer incidence and mortality worldwide: Sources, methods and major patterns in GLOBOCAN. 2012","volume":"136","author":"Bray","year":"2015","journal-title":"Int J Cancer"},{"key":"2","doi-asserted-by":"publisher","first-page":"5171","DOI":"10.3748\/wjg.v18.i37.5171","article-title":"KRAS mutation testing in metastatic colorectal cancer","volume":"18","author":"Du","year":"2012","journal-title":"World J Gastroenterol"},{"key":"3","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1200\/JCO.2010.30.1366","article-title":"Value of mismatch repair, KRAS, and BRAF mutations in predicting recurrence and benefits from chemotherapy in colorectal cancer","volume":"29","author":"Quirke","year":"2011","journal-title":"J Clin Oncol"},{"key":"4","doi-asserted-by":"publisher","first-page":"3206","DOI":"10.18632\/oncotarget.13884","article-title":"KRAS mutant colorectal cancer gene signatures identified angiotensin II receptor blockers as potential therapies","volume":"8","author":"Zhang","year":"2017","journal-title":"Oncotarget"},{"key":"5","doi-asserted-by":"publisher","first-page":"22257","DOI":"10.18632\/oncotarget.8076","article-title":"Mutations of KRAS\/NRAS\/BRAF predict cetuximab resistance in metastatic colorectal cancer patients","volume":"7","author":"Yang","year":"2016","journal-title":"Oncotarget"},{"key":"6","doi-asserted-by":"publisher","first-page":"32134","DOI":"10.18632\/oncotarget.16739","article-title":"Metabolic profiling and novel plasma biomarkers for predicting survival in epithelial ovarian cancer","volume":"8","author":"Li","year":"2017","journal-title":"Oncotarget"},{"key":"7","doi-asserted-by":"crossref","unstructured":"Wong, Qian, Li, Xu, Kang, Tong, To, Jin, Li, Chen, Go, Wu, Cheng, et al. SLC25A22 Promotes Proliferation and Survival of Colorectal Cancer Cells With KRAS Mutations, and Xenograft Tumor Progression in Mice, via Intracellular Synthesis of Aspartate. Gastroenterology. 2016. https:\/\/doi.org\/10.1053\/j.gastro.2016.07.011.","DOI":"10.1053\/j.gastro.2016.07.011"},{"key":"8","doi-asserted-by":"publisher","first-page":"873","DOI":"10.1002\/ana.23998","article-title":"SLC25A22 is a novel gene for migrating partial seizures in infancy","volume":"74","author":"Bashiri","year":"2013","journal-title":"Ann Neurol"},{"key":"9","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1111\/j.1399-0004.2009.01236.x","article-title":"Mutations in the mitochondrial glutamate carrier SLC25A22 in neonatal epileptic encephalopathy with suppression bursts","volume":"76","author":"Colleaux","year":"2009","journal-title":"Clin Genet"},{"key":"10","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1038\/nrd.2016.32","article-title":"Emerging applications of metabolomics in drug discovery and precision medicine","volume":"15","author":"Wishart","year":"2016","journal-title":"Nat Rev Drug Discov"},{"key":"11","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1038\/nrneph.2011.152","article-title":"Metabolomics in the study of kidney diseases","volume":"8","author":"Kim","year":"2011","journal-title":"Nat Rev Nephrol"},{"key":"12","doi-asserted-by":"publisher","first-page":"300","DOI":"10.1038\/nchembio.1204","article-title":"NNMT promotes epigenetic remodeling in cancer by creating a metabolic methylation sink","volume":"9","author":"Cravatt","year":"2013","journal-title":"Nat Chem Biol"},{"key":"13","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1016\/j.cmet.2015.04.011","article-title":"Metabolism Links Bacterial Biofilms and Colon Carcinogenesis","volume":"21","author":"Casero","year":"2015","journal-title":"Cell Metabolism"},{"key":"14","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1038\/nrm3314","article-title":"Metabolomics: the apogee of the omics trilogy","volume":"13","author":"Siuzdak","year":"2012","journal-title":"Nat Rev Mol Cell Biol"},{"key":"15","doi-asserted-by":"publisher","first-page":"451","DOI":"10.1038\/nrm.2016.25","article-title":"Metabolomics: beyond biomarkers and towards mechanisms","volume":"17","author":"Siuzdak","year":"2016","journal-title":"Nat Rev Mol Cell Biol"},{"key":"16","doi-asserted-by":"publisher","first-page":"1483","DOI":"10.1038\/nprot.2011.375","article-title":"Global urinary metabolic profiling procedures using gas chromatography-mass spectrometry","volume":"6","author":"Nicholson","year":"2011","journal-title":"Nat Protoc"},{"key":"17","doi-asserted-by":"publisher","first-page":"285","DOI":"10.1016\/j.talanta.2016.10.013","article-title":"Determination of amino acids in colon cancer cells by using UHPLC-MS\/MS and [U-13C5]-glutamine as the isotope tracer","volume":"162","author":"Cai","year":"2017","journal-title":"Talanta"},{"key":"18","doi-asserted-by":"publisher","first-page":"707","DOI":"10.1016\/j.bbabio.2010.12.002","article-title":"AICAR inhibits cancer cell growth and triggers cell-type distinct effects on OXPHOS biogenesis, oxidative stress and Akt activation","author":"Rossignol","year":"2011","journal-title":"Biochimica et Biophysica Acta (BBA) - Bioenergetics"},{"key":"19","doi-asserted-by":"publisher","first-page":"1504","DOI":"10.1053\/j.gastro.2012.02.050","article-title":"Aspirin inhibits mTOR signaling, activates AMP-activated protein kinase, and induces autophagy in colorectal cancer cells","volume":"142","author":"Dunlop","year":"2012","journal-title":"Gastroenterology"},{"key":"20","doi-asserted-by":"publisher","first-page":"427","DOI":"10.1016\/j.tibs.2010.05.003","article-title":"Glutamine addiction: a new therapeutic target in cancer","volume":"35","author":"Thompson","year":"2010","journal-title":"Trends Biochem Sci"},{"key":"21","doi-asserted-by":"publisher","first-page":"362","DOI":"10.1016\/j.semcdb.2012.02.002","article-title":"Metabolic reprogramming in cancer: unraveling the role of glutamine in tumorigenesis","volume":"23","author":"Wellen","year":"2012","journal-title":"Semin Cell Dev Biol"},{"key":"22","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1038\/nature12040","article-title":"Glutamine supports pancreatic cancer growth through a KRAS-regulated metabolic pathway","volume":"496","author":"Bardeesy","year":"2013","journal-title":"Nature"},{"key":"23","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1038\/nrc1454","article-title":"Polyamines and cancer: old molecules, new understanding","volume":"4","author":"Meyskens","year":"2004","journal-title":"Nat Rev Cancer"},{"key":"24","doi-asserted-by":"publisher","first-page":"739","DOI":"10.1038\/nature08617","article-title":"Cancer-associated IDH1 mutations produce 2-hydroxyglutarate","volume":"462","author":"Ward","year":"2009","journal-title":"Nature"},{"key":"25","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/j.trac.2011.08.009","article-title":"Metabolite identification and quantitation in LC-MS\/MS-based metabolomics","volume":"32","author":"Ressom","year":"2012","journal-title":"Trends Analyt Chem"},{"key":"26","doi-asserted-by":"publisher","first-page":"872","DOI":"10.1038\/nprot.2012.024","article-title":"A positive\/negative ion-switching, targeted mass spectrometry-based metabolomics platform for bodily fluids, cells, and fresh and fixed tissue","volume":"7","author":"Asara","year":"2012","journal-title":"Nat Protoc"},{"key":"27","doi-asserted-by":"publisher","first-page":"4918","DOI":"10.1158\/0008-5472.CAN-08-4806","article-title":"Quantitative metabolome profiling of colon and stomach cancer microenvironment by capillary electrophoresis time-of-flight mass spectrometry","volume":"69","author":"Soga","year":"2009","journal-title":"Cancer Res"},{"key":"28","doi-asserted-by":"publisher","first-page":"e40459","DOI":"10.1371\/journal.pone.0040459","article-title":"A Novel Serum Metabolomics-Based Diagnostic Approach for Colorectal Cancer","volume":"7","author":"Yoshida","year":"2012","journal-title":"PLoS ONE"},{"key":"29","doi-asserted-by":"publisher","first-page":"72","DOI":"10.1186\/1476-4598-7-72","article-title":"Metabolite profiling of human colon carcinoma - deregulation of TCA cycle and amino acid turnover","volume":"7","author":"Fiehn","year":"2008","journal-title":"Molecular Cancer"},{"key":"30","doi-asserted-by":"publisher","first-page":"479","DOI":"10.1038\/nature19084","article-title":"Pancreatic stellate cells support tumour metabolism through autophagic alanine secretion","volume":"536","author":"Cantley","year":"2016","journal-title":"Nature"},{"key":"31","doi-asserted-by":"publisher","first-page":"11457","DOI":"10.1038\/ncomms11457","article-title":"Asparagine promotes cancer cell proliferation through use as an amino acid exchange factor","volume":"7","author":"Christofk","year":"2016","journal-title":"Nat Commun"},{"key":"32","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1016\/j.neo.2016.09.004","article-title":"Metabolic alterations caused by KRAS mutations in colorectal cancer contribute to cell adaptation to glutamine depletion by upregulation of asparagine synthetase","volume":"18","author":"Sakai","year":"2016","journal-title":"Neoplasia"},{"key":"33","doi-asserted-by":"publisher","first-page":"411","DOI":"10.3945\/ajcn.114.103895","article-title":"Dietary polyamine intake and colorectal cancer risk in postmenopausal women","volume":"102","author":"Thompson","year":"2015","journal-title":"Am J Clin Nutrition"},{"key":"34","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1186\/1756-9966-30-95","article-title":"The mechanisms by which polyamines accelerate tumor spread","volume":"30","author":"Soda","year":"2011","journal-title":"J Exp Clin Cancer Res"},{"key":"35","doi-asserted-by":"publisher","first-page":"15354","DOI":"10.1073\/pnas.1010203108","article-title":"Polyamine catabolism contributes to enterotoxigenic Bacteroides fragilis-induced colon tumorigenesis","volume":"108","author":"Casero","year":"2011","journal-title":"Proc Nat Aca Sci USA"},{"key":"36","doi-asserted-by":"publisher","first-page":"1092","DOI":"10.18632\/oncotarget.13582","article-title":"Spermidine\/spermine N(1)-acetyltransferase regulates cell growth and metastasis via AKT\/\u03b2-catenin signaling pathways in hepatocellular and colorectal carcinoma cells","volume":"8","author":"Dai","year":"2017","journal-title":"Oncotarget"},{"key":"37","doi-asserted-by":"publisher","first-page":"1060","DOI":"10.1038\/nprot.2011.335","article-title":"Procedures for large-scale metabolic profiling of serum and plasma using gas chromatography and liquid chromatography coupled to mass spectrometry","volume":"6","author":"Kell","year":"2011","journal-title":"Nat Protocols"},{"key":"38","doi-asserted-by":"publisher","first-page":"779","DOI":"10.1021\/ac051437y","article-title":"XCMS: processing mass spectrometry data for metabolite profiling using nonlinear peak alignment, matching, and identification","volume":"78","author":"Siuzdak","year":"2006","journal-title":"Analytical Chemistry"},{"key":"39","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1021\/ac051495j","article-title":"Large-scale human metabolomics studies: a strategy for data (pre-) processing and validation","volume":"78","author":"Smilde","year":"2006","journal-title":"Analytical Chemistry"},{"key":"40","doi-asserted-by":"publisher","first-page":"1338","DOI":"10.1373\/clinchem.2012.200527","article-title":"Fasting serum lipid and dehydroepiandrosterone sulfate as important metabolites for detecting isolated postchallenge diabetes: serum metabolomics via ultra-high-performance LC-MS","volume":"59","author":"Sun","year":"2013","journal-title":"Clin Chem"},{"key":"41","doi-asserted-by":"publisher","first-page":"1401","DOI":"10.1002\/cpbi.11","article-title":"Using MetaboAnalyst 3.0 for comprehensive metabolomics data analysis","volume":"55","author":"Wishart","year":"2016","journal-title":"Curr Protoc Bioinformatics"}],"container-title":["Oncotarget"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.oncotarget.com\/lookup\/doi\/10.18632\/oncotarget.21093","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,6]],"date-time":"2020-08-06T18:05:09Z","timestamp":1596737109000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.oncotarget.com\/lookup\/doi\/10.18632\/oncotarget.21093"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,20]]},"references-count":41,"journal-issue":{"issue":"60","published-print":{"date-parts":[[2017,11,24]]}},"alternative-id":["21093","29254168"],"URL":"https:\/\/doi.org\/10.18632\/oncotarget.21093","relation":{},"ISSN":["1949-2553"],"issn-type":[{"value":"1949-2553","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,20]]}}}