{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T13:41:48Z","timestamp":1792676508215,"version":"4.5.0"},"reference-count":33,"publisher":"Impact Journals, LLC","issue":"50","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncotarget"],"published-print":{"date-parts":[[2017,10,20]]},"DOI":"10.18632\/oncotarget.21256","type":"journal-article","created":{"date-parts":[[2017,9,24]],"date-time":"2017-09-24T08:34:36Z","timestamp":1506242076000},"page":"87980-87996","source":"Crossref","is-referenced-by-count":33,"title":["Evaluation of pre-analytical conditions and comparison of the performance of several digital PCR assays for the detection of major <i>EGFR<\/i> mutations in circulating DNA from non-small cell lung cancers: the CIRCAN_0 study"],"prefix":"10.18632","volume":"8","author":[{"given":"Jessica","family":"Garcia","sequence":"first","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 69310, Pierre B\u00e9nite, France"},{"name":"Centre de Recherche en Canc\u00e9rologie de Lyon, INSERM 1052, CNRS 5286, Universit\u00e9 Claude Bernard Lyon 1, Lyon, 69003, France"},{"name":"Laboratoire Commun de Recherche Hospices Civils de Lyon - BioM\u00e9rieux, Centre Hospitalier Lyon Sud, 69310, Pierre B\u00e9nite, France"},{"name":"Institut de Canc\u00e9rologie des Hospices Civils de Lyon, CIRculating CANcer Program (CIRCAN), 69002 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Dusserre","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 69310, Pierre B\u00e9nite, France"},{"name":"Laboratoire Commun de Recherche Hospices Civils de Lyon - BioM\u00e9rieux, Centre Hospitalier Lyon Sud, 69310, Pierre B\u00e9nite, France"},{"name":"Institut de Canc\u00e9rologie des Hospices Civils de Lyon, CIRculating CANcer Program (CIRCAN), 69002 Lyon, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Val\u00e9rie","family":"Cheynet","sequence":"additional","affiliation":[{"name":"Laboratoire Commun de Recherche Hospices Civils de Lyon - BioM\u00e9rieux, Centre Hospitalier Lyon Sud, 69310, Pierre B\u00e9nite, France"},{"name":"Medical Diagnostic Discovery Department, BioM\u00e9rieux, 69290 Craponne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre Paul","family":"Bringuier","sequence":"additional","affiliation":[{"name":"Service d'Anatomie et de Cytologie Pathologiques, Groupement Hospitalier Est, Hospices Civils de Lyon, 69500, Bron, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Brengle-Pesce","sequence":"additional","affiliation":[{"name":"Laboratoire Commun de Recherche Hospices Civils de Lyon - BioM\u00e9rieux, Centre Hospitalier Lyon Sud, 69310, Pierre B\u00e9nite, France"},{"name":"Medical Diagnostic Discovery Department, BioM\u00e9rieux, 69290 Craponne, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Sophie","family":"Wozny","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 69310, Pierre B\u00e9nite, France"},{"name":"Facult\u00e9 de Pharmacie de Lyon (IPSB), Universit\u00e9 de Lyon1, Lyon, 69008, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire","family":"Rodriguez-Lafrasse","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 69310, Pierre B\u00e9nite, France"},{"name":"Laboratoire Commun de Recherche Hospices Civils de Lyon - BioM\u00e9rieux, Centre Hospitalier Lyon Sud, 69310, Pierre B\u00e9nite, France"},{"name":"Institut de Canc\u00e9rologie des Hospices Civils de Lyon, CIRculating CANcer Program (CIRCAN), 69002 Lyon, France"},{"name":"Facult\u00e9 de Pharmacie de Lyon (IPSB), Universit\u00e9 de Lyon1, Lyon, 69008, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Freyer","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie des Hospices Civils de Lyon, CIRculating CANcer Program (CIRCAN), 69002 Lyon, France"},{"name":"Service d'Oncologie M\u00e9dicale, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, Lyon, 69003, France"},{"name":"EMR 3738 Ciblage Th\u00e9rapeutique en Oncologie, Facult\u00e9 de M\u00e9decine Lyon Sud, Universit\u00e9 Lyon 1, 69600, Oullins, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Brevet","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie des Hospices Civils de Lyon, CIRculating CANcer Program (CIRCAN), 69002 Lyon, France"},{"name":"Service d'Anatomie et de Cytologie Pathologiques, Groupement Hospitalier Est, Hospices Civils de Lyon, 69500, Bron, France"},{"name":"EMR 3738 Ciblage Th\u00e9rapeutique en Oncologie, Facult\u00e9 de M\u00e9decine Lyon Sud, Universit\u00e9 Lyon 1, 69600, Oullins, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L\u00e9a","family":"Payen","sequence":"additional","affiliation":[{"name":"Laboratoire de Biochimie et Biologie Mol\u00e9culaire, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, 69310, Pierre B\u00e9nite, France"},{"name":"Centre de Recherche en Canc\u00e9rologie de Lyon, INSERM 1052, CNRS 5286, Universit\u00e9 Claude Bernard Lyon 1, Lyon, 69003, France"},{"name":"Laboratoire Commun de Recherche Hospices Civils de Lyon - BioM\u00e9rieux, Centre Hospitalier Lyon Sud, 69310, Pierre B\u00e9nite, France"},{"name":"Institut de Canc\u00e9rologie des Hospices Civils de Lyon, CIRculating CANcer Program (CIRCAN), 69002 Lyon, France"},{"name":"Facult\u00e9 de Pharmacie de Lyon (IPSB), Universit\u00e9 de Lyon1, Lyon, 69008, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9bastien","family":"Couraud","sequence":"additional","affiliation":[{"name":"Institut de Canc\u00e9rologie des Hospices Civils de Lyon, CIRculating CANcer Program (CIRCAN), 69002 Lyon, France"},{"name":"EMR 3738 Ciblage Th\u00e9rapeutique en Oncologie, Facult\u00e9 de M\u00e9decine Lyon Sud, Universit\u00e9 Lyon 1, 69600, Oullins, France"},{"name":"Service de Pneumologie Aigue Sp\u00e9cialis\u00e9e et Canc\u00e9rologie Thoracique, Centre Hospitalier Lyon Sud, Hospices Civils de Lyon, Lyon, 69003, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7892","published-online":{"date-parts":[[2017,9,21]]},"reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1016\/S1470-2045(10)70087-5","article-title":"New driver mutations in non-small-cell lung cancer","volume":"12","author":"Girard","year":"2011","journal-title":"The Lancet Oncology"},{"key":"2","doi-asserted-by":"crossref","first-page":"v1","DOI":"10.1093\/annonc\/mdw326","article-title":"Metastatic non-small-cell lung cancer: ESMO clinical practice guidelines for diagnosis, treatment and follow-up","volume":"27","author":"Peters","year":"2016","journal-title":"Ann Oncol"},{"key":"3","doi-asserted-by":"crossref","first-page":"2240","DOI":"10.1158\/1078-0432.CCR-12-2246","article-title":"Analysis of tumor specimens at the time of acquired resistance to EGFR-TKI therapy in 155 patients with EGFR-mutant lung cancers","volume":"19","author":"Riely","year":"2013","journal-title":"Clin Cancer Res"},{"key":"4","doi-asserted-by":"crossref","first-page":"1689","DOI":"10.1056\/NEJMoa1411817","article-title":"AZD9291 in EGFR inhibitor-resistant non-small-cell lung cancer","volume":"372","author":"Kim","year":"2015","journal-title":"The New England journal of medicine"},{"key":"5","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1038\/nrclinonc.2012.42","article-title":"Tumour molecular profiling for deciding therapy-the French initiative","volume":"9","author":"Calvo","year":"2012","journal-title":"Nature reviews Clinical oncology"},{"key":"6","doi-asserted-by":"crossref","first-page":"1415","DOI":"10.1016\/S0140-6736(16)00004-0","article-title":"Routine molecular profiling of patients with advanced non-small-cell lung cancer: results of a 1-year nationwide programme of the French Cooperative Thoracic Intergroup (IFCT)","volume":"387","author":"Lemoine","year":"2016","journal-title":"Lancet"},{"key":"7","doi-asserted-by":"crossref","first-page":"411","DOI":"10.1097\/JTO.0b013e3181d57f6e","article-title":"Pathologic diagnosis of advanced lung cancer based on small biopsies and cytology: a paradigm shift","volume":"5","author":"Yatabe","year":"2010","journal-title":"Journal of thoracic oncology"},{"key":"8","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1038\/nrclinonc.2013.110","article-title":"Liquid biopsy: monitoring cancer-genetics in the blood","volume":"10","author":"Bardelli","year":"2013","journal-title":"Nature reviews Clinical oncology"},{"key":"9","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1038\/nrc3066","article-title":"Cell-free nucleic acids as biomarkers in cancer patients","volume":"11","author":"Pantel","year":"2011","journal-title":"Nature reviews Cancer"},{"key":"10","doi-asserted-by":"crossref","first-page":"579","DOI":"10.1200\/JCO.2012.45.2011","article-title":"Liquid biopsies: genotyping circulating tumor DNA","volume":"32","author":"Bardelli","year":"2014","journal-title":"Journal of clinical oncology"},{"key":"11","doi-asserted-by":"crossref","first-page":"4613","DOI":"10.1158\/1078-0432.CCR-13-3063","article-title":"Noninvasive diagnosis of actionable mutations by deep sequencing of circulating free DNA in lung cancer from never-smokers: a proof-of-concept study from BioCAST\/IFCT-1002","volume":"20","author":"Margery","year":"2014","journal-title":"Clin Cancer Res"},{"key":"12","doi-asserted-by":"publisher","first-page":"44583","DOI":"10.18632\/oncotarget.10069","article-title":"Circulating cell-free DNA as a prognostic and predictive biomarker in non-small cell lung cancer","volume":"7","author":"Peng","year":"2016","journal-title":"Oncotarget"},{"key":"13","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1016\/j.cca.2013.05.022","article-title":"Circulating cell free DNA: preanalytical considerations","volume":"424","author":"Thierry","year":"2013","journal-title":"Clin Chim Acta"},{"key":"14","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.cca.2015.08.028","article-title":"Cell-free DNA: preanalytical variables","volume":"450","author":"Pretorius","year":"2015","journal-title":"Clin Chim Acta"},{"key":"15","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1634\/theoncologist.2015-0288","article-title":"Picoliter-droplet digital polymerase chain reaction-based analysis of cell-free plasma DNA to assess EGFR mutations in lung adenocarcinoma that confer resistance to tyrosine-kinase inhibitors","volume":"21","author":"Kuwano","year":"2016","journal-title":"The oncologist"},{"key":"16","doi-asserted-by":"crossref","first-page":"629","DOI":"10.1056\/NEJMoa1612674","article-title":"Osimertinib or Platinium-Pemetrexed in EGFR T790M-Positive Lung Cancer","volume":"376","author":"Mok","year":"2017","journal-title":"The New England Journal Of Medicine"},{"key":"17","first-page":"225","article-title":"Recommendations by the GFCO for the diagnostic use of somatic genetic analyses of circulating tumour DNA","volume":"2","author":"Rouleau","year":"2016","journal-title":"Innov Therap Oncol"},{"key":"18","doi-asserted-by":"crossref","first-page":"305","DOI":"10.1002\/jcla.21603","article-title":"A new blood collection device minimizes cellular DNA release during sample storage and shipping when compared to a standard device","volume":"27","author":"Fernando","year":"2013","journal-title":"J Clin Lab Anal"},{"key":"19","doi-asserted-by":"crossref","first-page":"e0150197","DOI":"10.1371\/journal.pone.0150197","article-title":"Optimised pre-analytical methods improve KRAS mutation detection in circulating tumour DNA (ctDNA) from patients with non-small cell lung cancer (NSCLC)","volume":"11","author":"Kohlmann","year":"2016","journal-title":"PLoS One"},{"key":"20","doi-asserted-by":"crossref","first-page":"1354","DOI":"10.1016\/j.clinbiochem.2016.03.012","article-title":"Comparative analysis of circulating tumor DNA stability In K3EDTA, Streck, and CellSave blood collection tubes","volume":"49","author":"Tewari","year":"2016","journal-title":"Clin Biochem"},{"key":"21","article-title":"A new blood collection tube and automated extraction method for stabilization and analysis of ccfDNA","author":"Gr\u00f6lz","year":"2015","journal-title":"Qiagen Website"},{"key":"22","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/j.jmoldx.2015.01.004","article-title":"Highly sensitive droplet digital PCR method for detection of EGFR-activating mutations in plasma cell-free DNA from patients with advanced non-small cell lung cancer","volume":"17","author":"Liu","year":"2015","journal-title":"J Mol Diagn"},{"key":"23","doi-asserted-by":"crossref","first-page":"1698","DOI":"10.1158\/1078-0432.CCR-13-2482","article-title":"Noninvasive detection of response and resistance in EGFR-mutant lung cancer using quantitative next-generation genotyping of cell-free plasma DNA","volume":"20","author":"Janne","year":"2014","journal-title":"Clin Cancer Res"},{"key":"24","doi-asserted-by":"crossref","first-page":"e0146275","DOI":"10.1371\/journal.pone.0146275","article-title":"Individualized mutation detection in circulating tumor DNA for monitoring colorectal tumor burden using a cancer-associated gene sequencing panel","volume":"11","author":"Nishizuka","year":"2016","journal-title":"PLoS One"},{"key":"25","doi-asserted-by":"crossref","first-page":"509","DOI":"10.1016\/j.lungcan.2015.10.004","article-title":"EGFR mutation detection in ctDNA from NSCLC patient plasma: a cross-platform comparison of leading technologies to support the clinical development of AZD9291","volume":"90","author":"Barrett","year":"2015","journal-title":"Lung Cancer"},{"key":"26","doi-asserted-by":"crossref","first-page":"982","DOI":"10.1001\/jamaoncol.2015.1066","article-title":"Acquired Resistance of EGFR-Mutant Lung Cancer to a T790M-Specific EGFR Inhibitor: Emergence of a Third Mutation (C797S) in the EGFR Tyrosine Kinase Domain","volume":"1","author":"Ladanyi","year":"2015","journal-title":"JAMA oncology"},{"key":"27","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.ymeth.2012.08.001","article-title":"Digital PCR strategies in the development and analysis of molecular biomarkers for personalized medicine","volume":"59","author":"McCaughan","year":"2013","journal-title":"Methods"},{"key":"28","doi-asserted-by":"crossref","first-page":"3276","DOI":"10.1158\/1078-0432.CCR-12-3768","article-title":"Noninvasive detection of HER2 amplification with plasma DNA digital PCR","volume":"19","author":"Turner","year":"2013","journal-title":"Clin Cancer Res"},{"key":"29","doi-asserted-by":"crossref","first-page":"6499","DOI":"10.1007\/s00216-014-7835-3","article-title":"Towards standardisation of cell-free DNA measurement in plasma: controls for extraction efficiency, fragment size bias and quantification","volume":"406","author":"Huggett","year":"2014","journal-title":"Anal Bioanal Chem"},{"key":"30","doi-asserted-by":"crossref","first-page":"3552","DOI":"10.1158\/1078-0432.CCR-14-2151","article-title":"Ultra-sensitive detection of the pretreatment EGFR T790M mutation in non-small cell lung cancer patients with an EGFR-activating mutation using droplet digital PCR","volume":"21","author":"Kataoka","year":"2015","journal-title":"Clin Cancer Res"},{"key":"31","first-page":"1096","article-title":"Early intervention in lung cancers with rapid plasma genotyping for EGFR and KRAS mutations-reply","volume":"2","author":"Oxnard","year":"2016","journal-title":"JAMA oncology"},{"key":"32","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1373\/clinchem.2014.222679","article-title":"Circulating tumor DNA as a liquid biopsy for cancer","volume":"61","author":"Geigl","year":"2015","journal-title":"Clin Chem"},{"key":"33","doi-asserted-by":"crossref","first-page":"e1006162","DOI":"10.1371\/journal.pgen.1006162","article-title":"Fragment Length of Circulating Tumor DNA","volume":"12","author":"Shendure","year":"2016","journal-title":"PLoS Genet"}],"container-title":["Oncotarget"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.oncotarget.com\/lookup\/doi\/10.18632\/oncotarget.21256","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,11]],"date-time":"2020-05-11T13:31:35Z","timestamp":1589203895000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.oncotarget.com\/lookup\/doi\/10.18632\/oncotarget.21256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,9,21]]},"references-count":33,"journal-issue":{"issue":"50","published-print":{"date-parts":[[2017,10,20]]}},"alternative-id":["21256","29152135"],"URL":"https:\/\/doi.org\/10.18632\/oncotarget.21256","relation":{},"ISSN":["1949-2553"],"issn-type":[{"value":"1949-2553","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,9,21]]}}}