{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T03:27:40Z","timestamp":1774668460150,"version":"3.50.1"},"reference-count":49,"publisher":"Impact Journals, LLC","issue":"18","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oncotarget"],"published-print":{"date-parts":[[2018,3,6]]},"DOI":"10.18632\/oncotarget.24537","type":"journal-article","created":{"date-parts":[[2018,2,20]],"date-time":"2018-02-20T15:20:29Z","timestamp":1519140029000},"page":"14492-14508","source":"Crossref","is-referenced-by-count":10,"title":["A pilot study on primary cultures of human respiratory tract epithelial cells to predict patients\u2019 responses to H7N9 infection"],"prefix":"10.18632","volume":"9","author":[{"given":"Chung-Guei","family":"Huang","sequence":"first","affiliation":[{"name":"Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Graduate Institute of Biomedical Sciences, Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li-Ang","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology - Head and Neck Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan, ROC"},{"name":"Faculty of Medicine, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi-Cheng","family":"Wu","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Department of Surgery, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33305, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mei-Jen","family":"Hsiao","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jim-Tong","family":"Horng","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Graduate Institute of Biomedical Sciences, Department of Biochemistry and Molecular Biology, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rei-Lin","family":"Kuo","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Graduate Institute of Biomedical Sciences, Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chih-Heng","family":"Huang","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Institute of Preventive Medicine, National Defense Medical Center, Taipei 11490, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ya-Chu","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuo-Chien","family":"Tsao","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min-Chi","family":"Chen","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Department of Public Health and Biostatistics Consulting Center, Chang Gung University, Taoyuan 33302, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tse-Ching","family":"Chen","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Department of Pathology, Linkou Chang Gung Memorial Hospital, Chang Gung University, Taoyuan 33305, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shin-Ru","family":"Shih","sequence":"additional","affiliation":[{"name":"Research Center for Emerging Viral Infections, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Graduate Institute of Biomedical Sciences, Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33302, Taiwan, ROC"},{"name":"Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan 33305, Taiwan, ROC"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7892","published-online":{"date-parts":[[2018,2,20]]},"reference":[{"key":"1","doi-asserted-by":"publisher","first-page":"1888","DOI":"10.1056\/NEJMoa1304459","article-title":"Human infection with a novel avian-origin influenza A (H7N9) virus","volume":"368","author":"Zhu","year":"2013","journal-title":"N Engl J Med"},{"key":"2","doi-asserted-by":"publisher","first-page":"2277","DOI":"10.1056\/NEJMoa1305584","article-title":"Clinical findings in 111 cases of influenza A (H7N9) virus infection","volume":"368","author":"Zhou","year":"2013","journal-title":"N Engl J Med"},{"key":"3","doi-asserted-by":"publisher","first-page":"6","DOI":"10.5365\/WPSAR.2017.8.1.001","article-title":"Sudden increase in human infection with avian influenza A(H7N9) virus in China, September-December 2016","volume":"8","author":"Feng","year":"2017","journal-title":"Western Pac Surveill Response J"},{"key":"4","doi-asserted-by":"publisher","first-page":"541","DOI":"10.1016\/s0140-6736(13)61904-2","article-title":"Effect of closure of live poultry markets on poultry-to-person transmission of avian influenza A H7N9 virus: an ecological study","volume":"383","author":"Feng","year":"2014","journal-title":"The Lancet"},{"key":"5","doi-asserted-by":"publisher","first-page":"410","DOI":"10.1126\/science.1240532","article-title":"H7N9 influenza viruses are transmissible in ferrets by respiratory droplet","volume":"341","author":"Liu","year":"2013","journal-title":"Science"},{"key":"6","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1186\/1743-422X-10-253","article-title":"The mouse and ferret models for studying the novel avian-origin human influenza A (H7N9) virus","volume":"10","author":"Li","year":"2013","journal-title":"Virol J"},{"key":"7","doi-asserted-by":"publisher","first-page":"560","DOI":"10.1038\/nature12476","article-title":"Limited airborne transmission of H7N9 influenza A virus between ferrets","volume":"501","author":"Burke","year":"2013","journal-title":"Nature"},{"key":"8","doi-asserted-by":"publisher","first-page":"500","DOI":"10.1038\/nature12379","article-title":"Biological features of novel avian influenza A (H7N9) virus","volume":"499","author":"Lan","year":"2013","journal-title":"Nature"},{"key":"9","doi-asserted-by":"publisher","first-page":"1137","DOI":"10.1016\/j.ajpath.2013.06.011","article-title":"Novel avian-origin influenza A (H7N9) virus attaches to epithelium in both upper and lower respiratory tract of humans","volume":"183","author":"Kuiken","year":"2013","journal-title":"Am J Pathol"},{"key":"10","doi-asserted-by":"publisher","first-page":"e00601","DOI":"10.1128\/mBio.00601-13","article-title":"The novel human influenza A(H7N9) virus is naturally adapted to efficient growth in human lung tissue","volume":"4","author":"Hippenstiel","year":"2013","journal-title":"MBio"},{"key":"11","doi-asserted-by":"publisher","first-page":"1","DOI":"10.5365\/WPSAR.2013.4.2.005","article-title":"Human infections with avian influenza A(H7N9) virus in China: preliminary assessments of the age and sex distribution","volume":"4","author":"Vong","year":"2013","journal-title":"Western Pac Surveill Response J"},{"key":"12","doi-asserted-by":"publisher","first-page":"e73362","DOI":"10.1371\/journal.pone.0073362","article-title":"Clinical features and factors associated with outcomes of patients infected with a Novel Influenza A (H7N9) virus: a preliminary study","volume":"8","author":"Chen","year":"2013","journal-title":"PLoS One"},{"key":"13","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1093\/cid\/ciu423","article-title":"Risk factors for influenza A(H7N9) disease--China, 2013","volume":"59","author":"Zhou","year":"2014","journal-title":"Clin Infect Dis"},{"key":"14","doi-asserted-by":"publisher","first-page":"1833","DOI":"10.1017\/S0950268814002866","article-title":"Prognosis and survival of 128 patients with severe avian influenza A(H7N9) infection in Zhejiang province, China","volume":"143","author":"He","year":"2015","journal-title":"Epidemiol Infect"},{"key":"15","doi-asserted-by":"publisher","first-page":"e0120999","DOI":"10.1371\/journal.pone.0120999","article-title":"Age and gender adjusted comparison of clinical features between severe cases infected with H7N9 and H1N1pdm influenza A in Jiangsu Province, China","volume":"10","author":"Bao","year":"2015","journal-title":"PLoS One"},{"key":"16","doi-asserted-by":"publisher","first-page":"236","DOI":"10.1093\/gerona\/glr248","article-title":"Mechanisms of dysfunction in senescent pulmonary endothelium","volume":"67","author":"Chun","year":"2012","journal-title":"J Gerontol A Biol Sci Med Sci"},{"key":"17","doi-asserted-by":"publisher","first-page":"O493","DOI":"10.1111\/1469-0691.12505","article-title":"Host immunological response and factors associated with clinical outcome in patients with the novel influenza A H7N9 infection","volume":"20","author":"Zhang","year":"2014","journal-title":"Clin Microbiol Infect"},{"key":"18","doi-asserted-by":"publisher","first-page":"769","DOI":"10.1073\/pnas.1321748111","article-title":"Early hypercytokinemia is associated with interferon-induced transmembrane protein-3 dysfunction and predictive of fatal H7N9 infection","volume":"111","author":"Yuan","year":"2014","journal-title":"Proc Natl Acad Sci U S A"},{"key":"19","doi-asserted-by":"publisher","first-page":"e0117846","DOI":"10.1371\/journal.pone.0117846","article-title":"Clinical, virological and immunological features from patients infected with re-emergent avian-origin human H7N9 influenza disease of varying severity in Guangdong province","volume":"10","author":"Huang","year":"2015","journal-title":"PLoS One"},{"key":"20","doi-asserted-by":"publisher","first-page":"10942","DOI":"10.1038\/srep10942","article-title":"The serum profile of hypercytokinemia factors identified in H7N9-infected patients can predict fatal outcomes","volume":"5","author":"Gao","year":"2015","journal-title":"Sci Rep"},{"key":"21","doi-asserted-by":"publisher","first-page":"1184","DOI":"10.1086\/656740","article-title":"The infection attack rate and severity of 2009 pandemic H1N1 influenza in Hong Kong","volume":"51","author":"Lo","year":"2010","journal-title":"Clin Infect Dis"},{"key":"22","doi-asserted-by":"publisher","first-page":"534","DOI":"10.1016\/s2213-2600(13)70138-3","article-title":"Tropism and innate host responses of a novel avian influenza A H7N9 virus: an analysis ofandcultures of the human respiratory tract","volume":"1","author":"Peiris","year":"2013","journal-title":"Lancet Respir Med"},{"key":"23","doi-asserted-by":"publisher","first-page":"e1789","DOI":"10.3791\/1789","article-title":"Primary human bronchial epithelial cells grown from explants","volume":"37","author":"Dolovich","year":"2010","journal-title":"J Vis Exp"},{"key":"24","doi-asserted-by":"publisher","first-page":"627","DOI":"10.1038\/nm.2358","article-title":"Molecular modeling, organ culture and reverse genetics for a newly identified human rhinovirus C","volume":"17","author":"Gern","year":"2011","journal-title":"Nat Med"},{"key":"25","doi-asserted-by":"publisher","first-page":"842","DOI":"10.1164\/rccm.201304-0750OC","article-title":"Relative respiratory syncytial virus cytopathogenesis in upper and lower respiratory tract epithelium","volume":"188","author":"Power","year":"2013","journal-title":"Am J Respir Crit Care Med"},{"key":"26","doi-asserted-by":"publisher","first-page":"3287","DOI":"10.1021\/acs.jproteome.7b00286","article-title":"Impact of influenza A virus infection on the proteomes of human bronchoepithelial cells from different donors","volume":"16","author":"Eichelberger","year":"2017","journal-title":"J Proteome Res"},{"key":"27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1111\/j.1750-2659.2009.00118.x","article-title":"Phenotypic characteristics of novel swine-origin influenza A\/California\/07\/2009 (H1N1) virus","volume":"4","author":"Rudenko","year":"2010","journal-title":"Influenza Other Respir Viruses"},{"key":"28","doi-asserted-by":"publisher","first-page":"e0148432","DOI":"10.1371\/journal.pone.0148432","article-title":"Genomic signatures for avian H7N9 viruses adapting to humans","volume":"11","author":"Tsao","year":"2016","journal-title":"PLoS One"},{"key":"29","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1128\/MMBR.05015-11","article-title":"Into the eye of the cytokine storm","volume":"76","author":"Katze","year":"2012","journal-title":"Microbiol Mol Biol Rev"},{"key":"30","doi-asserted-by":"publisher","first-page":"2109","DOI":"10.1183\/09031936.94.07122109","article-title":"Effect of Haemophilus influenzae endotoxin on the synthesis of IL-6, IL-8, TNF-alpha and expression of ICAM-1 in cultured human bronchial epithelial cells","volume":"7","author":"Davies","year":"1994","journal-title":"Eur Respir J"},{"key":"31","doi-asserted-by":"publisher","first-page":"S38","DOI":"10.1164\/ajrccm.160.supplement_1.11","article-title":"Airway epithelial cells, cytokines, and pollutants","volume":"160","author":"Devalia","year":"1999","journal-title":"Am J Respir Crit Care Med"},{"key":"32","doi-asserted-by":"publisher","first-page":"1882","DOI":"10.1016\/j.biocel.2003.10.019","article-title":"Macrophage inflammatory protein-1","volume":"36","author":"von Stebut","year":"2004","journal-title":"Int J Biochem Cell Biol"},{"key":"33","doi-asserted-by":"publisher","first-page":"16","DOI":"10.1186\/s12950-015-0054-7","article-title":"Human parainfluenza virus type 3 (HPIV3) induces production of IFNgamma and RANTES in human nasal epithelial cells (HNECs)","volume":"12","author":"Kowalski","year":"2015","journal-title":"J Inflamm (Lond)"},{"key":"34","doi-asserted-by":"publisher","first-page":"e114322","DOI":"10.1371\/journal.pone.0114322","article-title":"Human rhinovirus induced cytokine\/chemokine responses in human airway epithelial and immune cells","volume":"9","author":"Anderson","year":"2014","journal-title":"PLoS One"},{"key":"35","doi-asserted-by":"publisher","first-page":"22","DOI":"10.1111\/j.1467-789X.2007.00434.x","article-title":"Obesity pandemic, correlated factors and guidelines to define, screen and manage obesity in Taiwan","volume":"9","author":"Fu","year":"2008","journal-title":"Obes Rev"},{"key":"36","doi-asserted-by":"publisher","first-page":"13","DOI":"10.1093\/biomet\/73.1.13","article-title":"Longitudinal data analysis using generalized linear models","volume":"73","author":"Zeger","year":"1986","journal-title":"Biometrika"},{"key":"37","doi-asserted-by":"publisher","first-page":"1962","DOI":"10.1093\/infdis\/jit440","article-title":"Cytokine and chemokine levels in patients infected with the novel avian influenza A (H7N9) virus in China","volume":"208","author":"Qi","year":"2013","journal-title":"J Infect Dis"},{"key":"38","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1164\/rccm.200407-857OC","article-title":"Characterization of cytokine\/chemokine profiles of severe acute respiratory syndrome","volume":"171","author":"Deng","year":"2005","journal-title":"Am J Respir Crit Care Med"},{"key":"39","doi-asserted-by":"publisher","first-page":"12982","DOI":"10.1128\/JVI.01571-14","article-title":"Human H7N9 and H5N1 influenza viruses differ in induction of cytokines and tissue tropism","volume":"88","author":"Schultz-Cherry","year":"2014","journal-title":"J Virol"},{"key":"40","doi-asserted-by":"publisher","first-page":"S178","DOI":"10.1086\/317513","article-title":"Circulating cytokines as mediators of fever","volume":"31","author":"Van der Meer","year":"2000","journal-title":"Clin Infect Dis"},{"key":"41","doi-asserted-by":"publisher","first-page":"837","DOI":"10.1016\/j.femsre.2004.12.003","article-title":"Role of apoptosis and cytokines in influenza virus morbidity","volume":"29","author":"Sweet","year":"2005","journal-title":"FEMS Microbiol Rev"},{"key":"42","doi-asserted-by":"publisher","first-page":"4655","DOI":"10.1128\/JVI.03095-14","article-title":"A(H7N9) virus results in early induction of proinflammatory cytokine responses in both human lung epithelial and endothelial cells and shows increased human adaptation compared with avian H5N1 virus","volume":"89","author":"Tumpey","year":"2015","journal-title":"J Virol"},{"key":"43","doi-asserted-by":"publisher","first-page":"R201","DOI":"10.1186\/cc8208","article-title":"Th1 and Th17 hypercytokinemia as early host response signature in severe pandemic influenza","volume":"13","author":"Tenorio-Abreu","year":"2009","journal-title":"Crit Care"},{"key":"44","doi-asserted-by":"publisher","first-page":"1463","DOI":"10.1183\/09031936.00186214","article-title":"Influenza virus-induced lung injury: pathogenesis and implications for treatment","volume":"45","author":"Budinger","year":"2015","journal-title":"Eur Respir J"},{"key":"45","doi-asserted-by":"publisher","first-page":"450","DOI":"10.3389\/fimmu.2016.00450","article-title":"Immunosenescence-related transcriptomic and immunologic changes in older individuals following influenza vaccination","volume":"7","author":"Poland","year":"2016","journal-title":"Front Immunol"},{"key":"46","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1186\/1742-4682-10-30","article-title":"How to interpret the transmissibility of novel influenza A(H7N9): an analysis of initial epidemiological data of human cases from China","volume":"10","author":"Ejima","year":"2013","journal-title":"Theor Biol Med Model"},{"key":"47","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/s1473-3099(13)70286-x","article-title":"Pathogenesis of influenza-induced acute respiratory distress syndrome","volume":"14","author":"Kuiken","year":"2014","journal-title":"Lancet Infect Dis"},{"key":"48","doi-asserted-by":"publisher","first-page":"2373","DOI":"10.1084\/jem.20140116","article-title":"Adiposity induces lethal cytokine storm after systemic administration of stimulatory immunotherapy regimens in aged mice","volume":"211","author":"Hesdorffer","year":"2014","journal-title":"J Exp Med"},{"key":"49","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1186\/1471-2334-11-232","article-title":"Direct association between pharyngeal viral secretion and host cytokine response in severe pandemic influenza","volume":"11","author":"Loza","year":"2011","journal-title":"BMC Infect Dis"}],"container-title":["Oncotarget"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.oncotarget.com\/lookup\/doi\/10.18632\/oncotarget.24537","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,9]],"date-time":"2020-05-09T15:01:47Z","timestamp":1589036507000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.oncotarget.com\/lookup\/doi\/10.18632\/oncotarget.24537"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,2,20]]},"references-count":49,"journal-issue":{"issue":"18","published-print":{"date-parts":[[2018,3,6]]}},"alternative-id":["24537","29581859"],"URL":"https:\/\/doi.org\/10.18632\/oncotarget.24537","relation":{},"ISSN":["1949-2553"],"issn-type":[{"value":"1949-2553","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,2,20]]}}}