{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T18:33:33Z","timestamp":1778610813439,"version":"3.51.4"},"reference-count":26,"publisher":"Institute of Cytology and Genetics, SB RAS","issue":"2","license":[{"start":{"date-parts":[[2021,4,29]],"date-time":"2021-04-29T00:00:00Z","timestamp":1619654400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/vavilov.elpub.ru\/jour\/about\/editorialPolicies#openAccessPolicy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vestn. VOGiS"],"abstract":"<jats:p>72 clinical strains of<jats:italic>Klebsiella<\/jats:italic>spp. isolated from samples obtained from humans in Novosibirsk, Russia, were analyzed. Species identification of strains was performed using 16S rRNA and<jats:italic>rpoB<\/jats:italic>gene sequences. It was revealed that<jats:italic>Klebsiella pneumoniae<\/jats:italic>strains were dominant in the population (57 strains), while the remaining 15 strains were<jats:italic>K. grimontii<\/jats:italic>,<jats:italic>K. aerogenes<\/jats:italic>,<jats:italic>K. oxytoca<\/jats:italic>and<jats:italic>K. quasipneumoniae<\/jats:italic>. By molecular serotyping using the<jats:italic>wzi<\/jats:italic>gene sequence,<jats:italic>K. pneumoniae<\/jats:italic>strains were assigned to twenty-one K-serotypes with a high proportion of virulent K1- and K2-serotypes. It was found that<jats:italic>K. pneumoniae<\/jats:italic>strains isolated from the hospitalized patients had a higher resistance to antibiotics compared to the other<jats:italic>Klebsiella<\/jats:italic>species. Real-time PCR revealed that the population contained genes of the<jats:italic>bla<\/jats:italic><jats:italic><jats:sub>SHV<\/jats:sub><\/jats:italic>,<jats:italic>bla<\/jats:italic><jats:italic><jats:sub>TEM<\/jats:sub><\/jats:italic>,<jats:italic>bla<\/jats:italic><jats:italic><jats:sub>CTX<\/jats:sub><\/jats:italic><jats:italic\/>families and the<jats:italic>bla<\/jats:italic><jats:italic><jats:sub>OXA-48<\/jats:sub><\/jats:italic><jats:italic\/>gene, which are the genetic determinants of beta-lactam resistance. It has been shown that the presence of the<jats:italic>bla<\/jats:italic><jats:italic><jats:sub>CTX<\/jats:sub><\/jats:italic>sequence correlated with the production of extended-spectrum beta-lactamases, and phenotypic resistance to car-bapenems is due to the presence of the<jats:italic>bla<\/jats:italic><jats:italic><jats:sub>OXA-48<\/jats:sub><\/jats:italic><jats:italic\/>gene. At the same time, the carbapenemase genes<jats:italic>vim<\/jats:italic>,<jats:italic>ndm<\/jats:italic>,<jats:italic>kpc<\/jats:italic>,<jats:italic>imp<\/jats:italic>were not detected. Among the aminoglycoside resistance genes studied, the<jats:italic>aph<\/jats:italic>(<jats:italic>6<\/jats:italic>)<jats:italic>-Id<\/jats:italic>and<jats:italic>aadA<\/jats:italic>genes were found, but their presence did not always coincide with phenotypic resistance. Resistance to fluoroquinolones in the vast majority of strains was accompanied by the presence of the<jats:italic>aac<\/jats:italic>(<jats:italic>6<\/jats:italic>\u2019)<jats:italic>-IB-cr<\/jats:italic>,<jats:italic>oqxA<\/jats:italic>,<jats:italic>oqxB<\/jats:italic>,<jats:italic>qnrB<\/jats:italic>, and<jats:italic>qnrS<\/jats:italic>genes in various combinations, while the presence of the<jats:italic>oqxA<\/jats:italic>and\/or<jats:italic>oqxB<\/jats:italic>genes alone did not correlate with resistance to fluoroquinolones. Thus, the detection of<jats:italic>bla<\/jats:italic><jats:italic><jats:sub>CTX<\/jats:sub><\/jats:italic><jats:italic\/>and<jats:italic>bla<\/jats:italic><jats:italic><jats:sub>OXA-48<\/jats:sub><\/jats:italic><jats:italic\/>can be used to quickly predict the production of extended-spectrum beta-lactamases and to determine the resistance of<jats:italic>Klebsiella<\/jats:italic>to carbapenems. The detection of the<jats:italic>aac<\/jats:italic>(<jats:italic>6<\/jats:italic>\u2019)<jats:italic>-Ib-cr<\/jats:italic>and\/or<jats:italic>qnrB<\/jats:italic>\/<jats:italic>qnrS<\/jats:italic>genes can be used to quickly determine resistance to fluoroquinolones.<\/jats:p>","DOI":"10.18699\/vj21.49-o","type":"journal-article","created":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T19:09:37Z","timestamp":1617044977000},"page":"234-245","source":"Crossref","is-referenced-by-count":2,"title":["Genetic characterization of clinical &lt;I&gt;Klebsiella&lt;\/I&gt; isolates circulating in Novosibirsk"],"prefix":"10.18699","volume":"25","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3872-4122","authenticated-orcid":true,"given":"A. V.","family":"Bardasheva","sequence":"first","affiliation":[{"name":"Institute of \u0421hemical Biology \u0430nd Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. V.","family":"Fomenko","sequence":"additional","affiliation":[{"name":"JSC \u201cVector-Best\u201d"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. V.","family":"Kalymbetova","sequence":"additional","affiliation":[{"name":"JSC \u201cVector-Best\u201d"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7158-3774","authenticated-orcid":true,"given":"I. V.","family":"Babkin","sequence":"additional","affiliation":[{"name":"Institute of \u0421hemical Biology \u0430nd Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. O.","family":"Chretien","sequence":"additional","affiliation":[{"name":"Novosibirsk Research Institute of Traumatology and Orthopedics named after Ya.L. Tsivyan of the Ministry of Health of the Russian Federation"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6787-8393","authenticated-orcid":true,"given":"E. V.","family":"Zhirakovskaya","sequence":"additional","affiliation":[{"name":"Institute of \u0421hemical Biology \u0430nd Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1687-8278","authenticated-orcid":true,"given":"N. V.","family":"Tikunova","sequence":"additional","affiliation":[{"name":"Institute of \u0421hemical Biology \u0430nd Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0869-3476","authenticated-orcid":true,"given":"V. V.","family":"Morozova","sequence":"additional","affiliation":[{"name":"Institute of \u0421hemical Biology \u0430nd Fundamental Medicine of Siberian Branch of the Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7950","published-online":{"date-parts":[[2021,4,29]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Ivanov D.V., Egorov A.M. Spreading and mechanisms of antimicrobial resistance in microorganisms, producing beta\u00adlactamases. Molecular mechanisms of resistance to beta\u00adlactam antibiotics of Klebsiella spp. strains, isolated in cases of nosocomial infections. Biochem. (Moscow) Suppl. Ser. B. 2008;2:311\u00ad317. DOI 10.1134\/S1990750808030141.","DOI":"10.1134\/S1990750808030141"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Kozlova N.S., Barantsevich N.E., Barantsevich E.P. Susceptibility to antibiotics in Klebsiella pneumoniae strains isolated in a multidisciplinary medical centre. Infektsiya i Immunitet = Russian Journal of Infection and Immunity. 2018;8(1):79\u00ad84. (in Russian)] DOI 10.15789\/2220\u00ad7619\u00ad2018\u00ad1\u00ad79\u00ad84.","DOI":"10.15789\/2220-7619-2018-1-79-84"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Kozlova Y.N., Fomenko N.V., Morozova V.V., Saranina I.V., Tikunov A.Yu., Ganichev D.A., Samokhin A.G., Pavlov V.V., Rozhnova O.M., Bondar\u2019 I.A., Zenkova E.V., Nimaev V.V., Klimontov V.V., Tikunova N.V. Genetic and biochemical characterization of staphylococci occurring in Novosibirsk, Russia. Vavilovskii Zhurnal Genetiki i Selektsii = Vavilov Journal of Genetics and Breeding. 2017; 21(8):952\u00ad958. DOI 10.18699\/VJ17.318. (in Russian)","DOI":"10.18699\/VJ17.318"},{"key":"ref4","unstructured":"Mashkovsky M.D. Medicinal Preparations. Moscow: Novaya Volna Publ., 2005. (in Russian)"},{"key":"ref5","unstructured":"Sukhorukova M.V., Edelstein M.V., Ivanchik N.V., Skleenova E.Yu., Shaidullina E.R., Azizov I.S., Shek E.A., KuzmenkovA.Iu., Dekhnich A.V., Kozlov R.S., Semenova N.V., \u2026 Zvonareva O.V., Kornilova P.A., Krianga V.G., Portniagina U.S., Shamaeva S.Kh., Popov D.A., Vostrikova T.Iu. Antimicrobial resistance of nosocomial Enterobacterales isolates in Russia: results of multicenter epidemiological study \u201cMARATHON 2015\u20132016\u201d. Klini\u010deska\u00e2 Mikrobiologi\u00e2 i Antimikrobna\u00e2 Himioterapi\u00e2 = Clinical Microbiology and Antimicrobial Chemotherapy. 2019;21(2):147\u00ad159. DOI 10.36488\/cmac.2019.2.147\u00ad159. (in Russian)"},{"key":"ref6","unstructured":"Tapalsky D.V., Osipov V.A., Zhavoronok S.V. Carbapenemases of gram\u00adnegative pathogens: spread and methods of detection. Meditsynskyj Zhurnal = Medical Journal. 2012;2(40):10\u00ad15. (in Russian)"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Chebotar I.V., Bocharova Yu.A., Podoprigora I.V., Shagin D.A. The reasons why Klebsiella pneumoniae becomes a leading opportunistic pathogen. Klini\u010deska\u00e2 Mikrobiologi\u00e2 i Antimikrobna\u00e2 Himioterapi\u00e2 = Clinical Microbiology and Antimicrobial Chemotherapy. 2020;22(1):4\u00ad19. DOI 10.36488\/cmac.2020.1.4\u00ad19. (in Russian)","DOI":"10.36488\/cmac.2020.1.4-19"},{"key":"ref8","unstructured":"Edelstein M.V. Detection of extended spectrum \u03b2\u00adlactamases by phenotypic methods in gram\u00adnegative bacteria. Klini\u010deska\u00e2 Mikrobiologi\u00e2 i Antimikrobna\u00e2 Himioterapi\u00e2 = Clinical Microbiology and Antimicrobial Chemotherapy. 2001;3(2):183\u00ad189. (in Russian)"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Brisse S., Passet V., Haugaard A.B., Babosan A., Kassis\u00adChikhani N., Struve C., Decr\u00e9 D. wzi gene sequencing, a rapid method for determination of capsulartype for Klebsiella strains. J. Clin. Microbiol. 2013;51:4073\u00ad4078. DOI 10.1128\/JCM.01924\u00ad13.","DOI":"10.1128\/JCM.01924-13"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Broberg C.A., Palacios M., Miller V.L. Klebsiella: a long way to go towards understanding this enigmatic jet\u00adsetter. F1000Prime Rep. 2014;6:64. DOI 10.12703\/P6\u00ad64.","DOI":"10.12703\/P6-64"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Bush K., Jacoby G.A. Updated functional classification of beta\u00adlactamases. Antimicrob. Agents Chemother. 2010;54(3):969\u00ad976. DOI 10.1128\/AAC.01009\u00ad09.","DOI":"10.1128\/AAC.01009-09"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Galal L., Abdel Aziz N.A., Hassan W.M. Defining the relationship between phenotypic and genotypic resistance profiles of multidrugresistant enterobacterial clinical isolates. Adv. Exp. Med. Biol. 2019; 1214:9\u00ad21. DOI 10.1007\/5584_2018_208.","DOI":"10.1007\/5584_2018_208"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Gharrah M.M., El\u00adMahdy A.M., Barwa R.F. Association between virulence factors and extended spectrum beta\u00adlactamase producing Kleb siella pneumoniae compared to nonproducing isolates. Interdiscip. Perspect. Infect. Dis. 2017:7279830. DOI 10.1155\/2017\/7279830.","DOI":"10.1155\/2017\/7279830"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Hoa P.L., Chowa K.H., Yuena K.Y., Ngb W.S., Chaua P.Y. Comparison of a novel, inhibitor\u00adpotentiated disc\u00addiffusion test with other methods for the detection of extended\u00adspectrum beta\u00adlactamases in Escherichia coli and Klebsiella pneumoniae. J. Antimicrob. Chemother. 1998;42:49\u00ad54.","DOI":"10.1093\/jac\/42.1.49"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Hooper D.C., Jacoby G.A. Mechanisms of drug resistance: quinolone resistance. Ann. N.Y. Acad. Sci. 2015;1354(1):12\u00ad31. DOI 10.1111\/nyas.12830.","DOI":"10.1111\/nyas.12830"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Lee H.C., Chuang Y.C., Yu W.L., Lee N.Y., Chang C.M., Ko N.Y., Wang L.R., Ko W.C. Clinical implications of hypermucoviscosity phenotype in Klebsiella pneumoniae isolates: association with invasive syndrome in patients with community\u00adacquired bacteraemia. J. Intern. Med. 2006;259(6):606\u00ad614. DOI 10.1111\/j.1365\u00ad2796.2006.01641.x.","DOI":"10.1111\/j.1365-2796.2006.01641.x"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Lee K., Chong Y., Shin H.B., Kim Y.A., Yong D., Yum J.H. Modified Hodge and EDTA\u00addisk synergy tests to screen metallo\u00adlactamaseproducing strains of Pseudomonas and Acinetobacter species. Clin. Microbiol. Infect. 2001;7:88\u00ad91.","DOI":"10.1046\/j.1469-0691.2001.00204.x"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Liakopoulos A., Mevius D., Ceccarelli D. A review of SHV extendedspectrum \u03b2\u00adlactamases: neglected yet ubiquitous. Front. Microbiol. 2016;5(7):1374. DOI 10.3389\/fmicb.2016.01374.","DOI":"10.3389\/fmicb.2016.01374"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Morozova V.V., Babkin I.V., Kozlova Y.N., Baykov I.K., Bokovaya O.V., Tikunov A.Yu., Ushakova T.\u0410., Bardasheva A.V., Ryabchikova E.I., Zelentsova E., Tikunova N.V. Isolation and characterization of a novel Klebsiella pneumoniae N4\u00adlike bacteriophage KP8. Viruses. 2019;11(12):1115. DOI 10.3390\/v11121115.","DOI":"10.3390\/v11121115"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Mukherjee S., Naha S., Bhadury P., Saha B., Dutta M., Dutta S., Basu S. Emergence of OXA\u00ad232\u00adproducing hypervirulent Klebsiella pneumoniae ST23 causing neonatal sepsis. J. Antimicrob. Chemother. 2020;75(7):2004\u00ad2006. DOI 10.1093\/jac\/dkaa080.","DOI":"10.1093\/jac\/dkaa080"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Podschun R., Ullmann U. Klebsiella spp. as nosocomial pathogens: epidemiology, taxonomy, typing methods, and pathogenicity factors. Clin. Microbiol. Rev. 1998;11(4):589\u00ad603.","DOI":"10.1128\/CMR.11.4.589"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Ramirez M.S., Tolmasky M.E. Aminoglycoside modifying enzymes. Drug. Resist. Updat. 2010;13(6):151\u00ad171.","DOI":"10.1016\/j.drup.2010.08.003"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Robicsek A., Jacoby G.A., Hooper D.C. The worldwide emergence of plasmid\u00admediated quinolone resistance. Lancet Infect. Dis. 2006;6: 629\u00ad640.","DOI":"10.1016\/S1473-3099(06)70599-0"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Walsh T.R., Toleman M.A., Poirel L., Nordmann P. Metallo\u00adbeta\u00adlactamases: the quiet before the storm? Clin. Microbiol. Rev. 2005;18(2): 306\u00ad325.","DOI":"10.1128\/CMR.18.2.306-325.2005"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Yang H.Y., Nam Y.S., Lee H.J. Prevalence of plasmid\u00admediated quinolone resistance genes among ciprofloxacin\u00adnonsusceptible Escherichia coli and Klebsiella pneumoniae isolated from blood cultures in Korea. Can. J. Infect. Dis. Med. Microbiol. 2014;25(3): 163\u00ad169.","DOI":"10.1155\/2014\/329541"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Yong D., Lee K., Yum J.H., Shin H.B., Rossolini G.M., Chong Y. Imipenem\u00adEDTA disk method for differentiation of metallo\u00adbetalactamase\u00adproducing clinical isolates of Pseudomonas spp. and Acinetobacter spp. J. Clin. Microbiol. 2002;40(10):3798\u00ad3801.","DOI":"10.1128\/JCM.40.10.3798-3801.2002"}],"container-title":["Vavilov Journal of Genetics and Breeding"],"original-title":[],"link":[{"URL":"https:\/\/vavilov.elpub.ru\/jour\/article\/viewFile\/2887\/1501","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T20:24:27Z","timestamp":1671740667000},"score":1,"resource":{"primary":{"URL":"https:\/\/vavilov.elpub.ru\/jour\/article\/view\/2887"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,29]]},"references-count":26,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2021,4,29]]}},"URL":"https:\/\/doi.org\/10.18699\/vj21.49-o","relation":{},"ISSN":["2500-3259","2500-0462"],"issn-type":[{"value":"2500-3259","type":"electronic"},{"value":"2500-0462","type":"print"}],"subject":[],"published":{"date-parts":[[2021,4,29]]}}}