{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T10:39:04Z","timestamp":1760524744175,"version":"3.41.2"},"reference-count":27,"publisher":"Institute of Cytology and Genetics, SB RAS","issue":"4","license":[{"start":{"date-parts":[[2025,7,20]],"date-time":"2025-07-20T00:00:00Z","timestamp":1752969600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/vavilov.elpub.ru\/jour\/about\/editorialPolicies#openAccessPolicy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vestn. VOGiS"],"abstract":"<jats:p>Grafting with resistant rootstocks is one of the most effective methods to prevent soil-borne diseases, and it can influence vegetative growth, flowering, maturation periods, and fruit quality, thereby ensuring high yields. In this study, four species from the family Cucurbitaceae were tested as potential candidates for grafting cucumber and melon: <jats:italic>Cucurbita ficifolia <\/jats:italic>Bouch\u00e9, <jats:italic>Cucurbita moschata <\/jats:italic>L., <jats:italic>Cucurbita pepo <\/jats:italic>L. and <jats:italic>Cucurbita maxima <\/jats:italic>Duch. The study focused on the grafting methods that optimize growth parameters and the accumulation of hormones and vitamins in rootstock. The results indicated that <jats:italic>Cucurbita maxima <\/jats:italic>Duch. is the most suitable rootstock material for grafting to <jats:italic>Cucumis sativus <\/jats:italic>L. and <jats:italic>Cucumis melo <\/jats:italic>L., as it exhibited superior plant and root mass. Among the two grafting methods tested, the tongue approach (\u2018X\u2019) demonstrated the best results in terms of growth parameters and the accumulation of indole-3-acetic acid (IAA) and vitamins in the scion leaves. IAA and vitamin concentrations were measured using HPLC in grafted samples at 2, 4 and 6 weeks of age. In the \u2018X\u2019 method, IAA accumulation from the end of the second week was twice as high compared to control plants. This method also showed higher vitamin content, with increased levels of B vitamins and vitamin C at the end of the 4th week (25.2\u2013135.1 and 52.3\u201367.0 %, respectively), and vitamins A, E, D<jats:sub>3<\/jats:sub>, K starting from the 2nd week (1.5\u20132 times higher). Conversely, the insertion or slant cut grafting method (\u2018Y\u2019) did not show any significant increase in the analyzed parameters and was comparable to the control. The \u2018X\u2019 method for grafting both <jats:italic>Cucumis sativus <\/jats:italic>L. and <jats:italic>Cucumis melo <\/jats:italic>L. onto <jats:italic>Cucurbita maxima <\/jats:italic>Duch. plants demonstrated the best results and is recommended for production.<\/jats:p>","DOI":"10.18699\/vjgb-25-59","type":"journal-article","created":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T12:47:19Z","timestamp":1753274839000},"page":"559-567","source":"Crossref","is-referenced-by-count":1,"title":["Influence of selected rootstock on growth parameters, accumulation of IAA and vitamins in scions of Cucumis sativus L. and Cucumis melo L."],"prefix":"10.18699","volume":"29","author":[{"given":"A. Zh.","family":"Shoibekova","sequence":"first","affiliation":[{"name":"Kazakh National Agrarian Research University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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A.","family":"Serikbayeva","sequence":"additional","affiliation":[{"name":"Kazakh National Agrarian Research University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. R.","family":"Toishimanov","sequence":"additional","affiliation":[{"name":"Kazakh National Agrarian Research University;\r\nInstitute of Plant Biology and Biotechnology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"G. T.","family":"Bari","sequence":"additional","affiliation":[{"name":"Kazakh National Agrarian Research University"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7950","published-online":{"date-parts":[[2025,7,20]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Abbas F., Faried H.N., Akhtar G., Ullah S., Javed T., Shehzad M.A., Ziaf K., Razzaq K., Amin M., Wattoo F.M., Hafeez A., Rahimi M., Abeed A.H.A. Cucumber grafting on indigenous cucurbit landraces confers salt tolerance and improves fruit yield by enhancing morpho-physio-biochemical and ionic attributes. Sci Rep. 2023;13(1): 21697. doi 10.1038\/s41598-023-48947-z","DOI":"10.1038\/s41598-023-48947-z"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Albacete A., Mart\u00ednez-And\u00fajar C., Mart\u00ednez-P\u00e9rez A., Thompson A.J., Dodd I.C., P\u00e9rez-Alfocea F. Unravelling rootstock\u00d7scion interactions to improve food security. J Exp Bot. 2015;66(8):2211-2226. doi 10.1093\/jxb\/erv027","DOI":"10.1093\/jxb\/erv027"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Asensi-Fabado M.A., Munn\u00e9-Bosch S. Vitamins in plants: occurrence, biosynthesis and antioxidant function. Trends Plant Sci. 2010; 15(10):582-592. doi 10.1016\/j.tplants.2010.07.003","DOI":"10.1016\/j.tplants.2010.07.003"},{"key":"ref4","unstructured":"Aslam J., Mohajir M.S., Khan S.A., Khan A.Q. HPLC analysis of water-soluble vitamins (B1, B2, B3, B5, B6) in in vitro and ex vitro germinated chickpea (Cicer arietinum L.). Afr J Biotechnol. 2008; 7(14):2310-2314. doi 10.5897\/AJB2008.000-5058"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Balliu A., Sallaku G. Exogenous auxin improves root morphology and restores growth of grafted cucumber seedlings. Hortic Sci. 2017; 44(2):82-90. doi 10.17221\/53\/2016-HORTSCI","DOI":"10.17221\/53\/2016-HORTSCI"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Bantis F., Panteris E., Dangitsis C., Carrera E., Koukounaras A. Blue light promotes vascular reconnection, while red light boosts the physiological response and quality of grafted watermelon seedlings. Sci Rep. 2021;11(1):21754. doi 10.1038\/s41598-021-01158-w","DOI":"10.1038\/s41598-021-01158-w"},{"key":"ref7","unstructured":"Battal P., Tileklio\u011flu B. The effects of different mineral nutrients on the levels of cytokinins in maize (Zea mays L.). Turk J Bot. 2001; 25(3):123-130"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Bekhradi F., Kashi A.K., Delshad M. Effect of different cucurbits rootstocks on vegetative and yield of watermelon. Acta Hortic. 2009; 807:649-654. doi 10.17660\/ActaHortic.2009.807.97","DOI":"10.17660\/ActaHortic.2009.807.97"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Bunsangiam S., Thongpae N., Limtong S., Nantana S. Large scale production of indole-3-acetic acid and evaluation of the inhibitory effect of indole-3-acetic acid on weed growth. Sci Rep. 2021;11(1):13094. doi 10.1038\/s41598-021-92305-w","DOI":"10.1038\/s41598-021-92305-w"},{"key":"ref10","unstructured":"El-Eslamboly A.A.S.A., Deabes A.A.A. Grafting cucumber onto some rootstocks for controlling root-knot nematodes. Minufiya J Agric Res. 2014;39(3):1109-1129"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Farhadi A., Aroeii H., Nemati H., Salehi R., Giuffrida F. The effectiveness of different rootstocks for improving yield and growth of cucumber cultivated hydroponically in a greenhouse. Horticulturae. 2016;2(1):1. doi 10.3390\/horticulturae2010001","DOI":"10.3390\/horticulturae2010001"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Keskin N., Kaya O., Ates F., Turan M., Guti\u00e9rrez-Gamboa G. Drying grapes after the application of different dipping solutions: effects on hormones, minerals, vitamins, and antioxidant enzymes in G\u00f6k \u00dcz\u00fcm (Vitis vinifera L.) raisins. Plants. 2022;11(4):529. doi 10.3390\/plants11040529","DOI":"10.3390\/plants11040529"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Lam V.P., Lee M.H., Park J.S. Optimization of indole-3-acetic acid concentration in a nutrient solution for increasing bioactive compound accumulation and production of Agastache rugosa in a plant factory. Agriculture. 2020;10(8):343. doi 10.3390\/agriculture10080343","DOI":"10.3390\/agriculture10080343"},{"key":"ref14","doi-asserted-by":"crossref","unstructured":"Lee J.M., Kubotab C., Tsaoc S.J., Bied Z., Echevarriae P.H., Morraf L., Oda M. Current status of vegetable grafting: diffusion, grafting techniques, automation. Sci Hortic. 2010;127(2):93-105. doi 10.1016\/j.scienta.2010.08.003","DOI":"10.1016\/j.scienta.2010.08.003"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Li W., Fang C., Krishnan S., Chen J., Yu H., Murphy A.S., Merewitz E., Katin-Grazzini L., McAvoy R.J., Deng Z., Zale J., Li Y. Elevated auxin and reduced cytokinin contents in rootstocks improve their performance and grafting success. Plant Biotechnol J. 2017;15(12): 1556-1565. doi 10.1111\/pbi.12738","DOI":"10.1111\/pbi.12738"},{"key":"ref16","doi-asserted-by":"crossref","unstructured":"Li X., Sun Y., Yuan X., Ma Z., Hong Y., Chen S. Impact of Cucurbita moschata resistant rootstocks on Cucumis sativus fruit and Meloidogyne incognita development. Plant Dis. 2023;107(12):3851-3857. doi 10.1094\/PDIS-02-22-0319-RE","DOI":"10.1094\/PDIS-02-22-0319-RE"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Mart\u00ednez-Ballesta M.C., Alcaraz-Lupez C., Muries B., Mota-Cadenas C., Carvajal M. Physiological aspects of rootstock-scion interactions. Sci Hortic. 2010;127(2):112-118. doi 10.1016\/j.scienta.2010.08.002","DOI":"10.1016\/j.scienta.2010.08.002"},{"key":"ref18","doi-asserted-by":"crossref","unstructured":"Mauro R.P., P\u00e9rez-Alfocea F., Cookson S.J., Ollat N., Vitale A. Physiological and molecular aspects of plant rootstock-scion interactions. Front Plant Sci. 2022;13:852518. doi 10.3389\/fpls.2022.852518","DOI":"10.3389\/fpls.2022.852518"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Mozumder N.H.M.R., Akhter M.J., Anwara A.K., Rokibuzzaman M., Akhtaruzzaman M. Estimation of water-soluble vitamin B-complex in selected leafy and non-leafy vegetables by HPLC method. Orient J Chem. 2019;35(4):1344-1351. doi 10.13005\/ojc\/350414","DOI":"10.13005\/ojc\/350414"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"Noda K., Okuda H., Iwagaki I. Indole acetic acid and abscisic acid levels in new shoots and fibrous roots of citrus scion-rootstock combinations. Sci Hortic. 2000;84(3):245-254. doi 10.1016\/S0304-4238(99)00080-1","DOI":"10.1016\/S0304-4238(99)00080-1"},{"key":"ref21","doi-asserted-by":"crossref","unstructured":"Noor R.S., Wang Z., Umair M., Yaseen M., Ameen M., Rehman S.U., Khan M.U., Imran M., Ahmed W., Sun Y. Interactive effects of grafting techniques and scion-rootstocks combinations on vegetative growth, yield and quality of cucumber (Cucumis sativus L.). Agronomy. 2019;9(6):288. doi 10.3390\/agronomy9060288","DOI":"10.3390\/agronomy9060288"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Stefancic M., Stampar F., Osterc G. Influence of endogenous IAA levels and exogenous IBA on rooting and quality of leafy cuttings of Prunus \u2018GiSelA 5\u2019. J Hortic Sci Biotechnol. 2006;81(3):508-512. doi 10.1080\/14620316.2006.11512095","DOI":"10.1080\/14620316.2006.11512095"},{"key":"ref23","doi-asserted-by":"crossref","unstructured":"Stefancic M., Stampar F., Veberic R., Osterc G. The levels of IAA, IAAsp and some phenolics in cherry rootstock \u02bbGiSelA 5\u2019 leafy cuttings pretreated with IAA and IBA. Sci Hortic. 2007;112(4): 399-405. doi 10.1016\/j.scienta.2007.01.004","DOI":"10.1016\/j.scienta.2007.01.004"},{"key":"ref24","doi-asserted-by":"crossref","unstructured":"Tang J., Li Y., Zhang L., Mu J., Jiang Y., Fu H., Zhang Y., Cui H., Yu X., Ye Z. Biosynthetic pathways and functions of indole-3-acetic acid in microorganisms. Microorganisms. 2023;11(8):2077. doi 10.3390\/microorganisms11082077","DOI":"10.3390\/microorganisms11082077"},{"key":"ref25","doi-asserted-by":"crossref","unstructured":"Toporek S.M., Keinath A.P. Evaluating cucurbit rootstocks to prevent disease caused by Pythium aphanidermatum and P. myriotylum on watermelon. Plant Dis. 2020;104(11):3019-3025. doi 10.1094\/PDIS-03-20-0474-RE","DOI":"10.1094\/PDIS-03-20-0474-RE"},{"key":"ref26","doi-asserted-by":"crossref","unstructured":"Traka-Mavrona E., Koutsika-Sotiriou M., Pritsa T. Response of squash (Cucurbita spp.) as rootstock for melon (Cucumis melo L.). Sci Hortic. 2000;83(3-4):353-362. doi 10.1016\/S0304-4238(99)00088-6","DOI":"10.1016\/S0304-4238(99)00088-6"},{"key":"ref27","doi-asserted-by":"crossref","unstructured":"Tsaballa A., Xanthopoulou A., Madesis P., Tsaftaris A., Nianiou-Obeidat I. Vegetable grafting from a molecular point of view: the involvement of epigenetics in rootstock-scion interactions. Front Plant Sci. 2021;11:621999. doi 10.3389\/fpls.2020.621999","DOI":"10.3389\/fpls.2020.621999"}],"container-title":["Vavilov Journal of Genetics and Breeding"],"original-title":[],"link":[{"URL":"https:\/\/vavilov.elpub.ru\/jour\/article\/viewFile\/4681\/1963","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"}],"deposited":{"date-parts":[[2025,7,23]],"date-time":"2025-07-23T12:47:53Z","timestamp":1753274873000},"score":1,"resource":{"primary":{"URL":"https:\/\/vavilov.elpub.ru\/jour\/article\/view\/4681"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,20]]},"references-count":27,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2025,7,20]]}},"URL":"https:\/\/doi.org\/10.18699\/vjgb-25-59","relation":{},"ISSN":["2500-3259"],"issn-type":[{"type":"electronic","value":"2500-3259"}],"subject":[],"published":{"date-parts":[[2025,7,20]]}}}