{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T19:48:31Z","timestamp":1775677711297,"version":"3.50.1"},"reference-count":67,"publisher":"Institute of Cytology and Genetics, SB RAS","issue":"1","license":[{"start":{"date-parts":[[2026,3,5]],"date-time":"2026-03-05T00:00:00Z","timestamp":1772668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/vavilov.elpub.ru\/jour\/about\/editorialPolicies#openAccessPolicy"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Vestn. VOGiS"],"abstract":"<jats:p>\n                    In plants, the regulation of transgene transcription is typically achieved using chemical agents. A\u00a0 safe alternative to chemically induced systems may be optogenetic systems. The BphP1-QPAS1 system has distinct advantages over other optogenetic systems, as it is activated by near-infrared (NIR, 780\u00a0nm) light, which is beyond the spectrum of plant photoreceptors. This system is based on the use of a split transcription factor\u00a0(TF), consisting of the DNA-binding and dimerization domains of the yeast TF Gal4, fused to the QPAS1 component, along with the transactivation domain VP16 fused to BphP1. Under NIR light, BphP1 interacts with QPAS1, leading to the formation of the functional TF Gal4-VP16. A\u00a0primary obstacle to using optogenetic systems in plants is their undesired activation under white light, which is vital for normal plant growth. A\u00a0potential solution to this issue is temporarily removing one component of the split TF from the nucleus under white light. We modified the BphP1-QPAS1 system to activate reporter gene expression in\n                    <jats:italic>Nicotiana benthamiana<\/jats:italic>\n                    leaves using NIR light. We combined BphP1-QPAS1 with several variants of LOV domain-containing proteins activated by blue light (460\u2013480\u00a0 nm). The best results were achieved by combining the BphP1-QPAS1 system with the AsLOV2 domain, which carries the degron sequence RRRG at the C-terminal J\u03b1 helix and initiates the degradation of the chimeric protein NES-Gal4-QPAS1-AsLOV2-RRRG under white light. This modification induced the BphP1-QPAS1 system in tobacco leaves only under NIR light, but not in the dark or under white light. We believe that, in the future, the BphP1-QPAS1 system could be applied to enhance plant resistance to adverse environmental conditions, pests, and viral diseases.\n                  <\/jats:p>","DOI":"10.18699\/vjgb-26-03","type":"journal-article","created":{"date-parts":[[2026,4,8]],"date-time":"2026-04-08T18:52:52Z","timestamp":1775674372000},"page":"61-71","source":"Crossref","is-referenced-by-count":0,"title":["Modification of the BphP1-QPAS1 optogenetic system for gene expression regulation in Nicotiana benthamiana tobacco leaves using near-infrared light"],"prefix":"10.18699","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4264-8750","authenticated-orcid":true,"given":"E. S.","family":"Surkova","sequence":"first","affiliation":[{"name":"Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences; Novosibirsk State University"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4185-833X","authenticated-orcid":true,"given":"Y. A.","family":"Galimova","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0006-7454-8273","authenticated-orcid":true,"given":"N. V.","family":"Battulina","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7980-0753","authenticated-orcid":true,"given":"D. M.","family":"Motorina","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2189-5101","authenticated-orcid":true,"given":"E. S.","family":"Omelina","sequence":"additional","affiliation":[{"name":"Institute of Molecular and Cellular Biology of the Siberian Branch of the Russian Academy of Sciences"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"7950","published-online":{"date-parts":[[2026,3,5]]},"reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Andres J., Blomeier T., Zurbriggen M.D. Synthetic switches and regulatory circuits in plants. Plant Physiol. 2019;179(3):862-884. doi 10.1104\/pp.18.01362","DOI":"10.1104\/pp.18.01362"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Auldridge M.E., Forest K.T. 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