{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T06:45:01Z","timestamp":1785739501481,"version":"3.56.0"},"reference-count":19,"publisher":"Korean Rhinologic Society","issue":"2","license":[{"start":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T00:00:00Z","timestamp":1785456000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"name":"Korean Rhinologic Society"}],"content-domain":{"domain":["j-rhinology.org"],"crossmark-restriction":true},"short-container-title":["J Rhinol"],"published-print":{"date-parts":[[2026,7,31]]},"abstract":"<jats:p>Background and Objectives: Eosinophilic chronic rhinosinusitis with nasal polyps (ECRSwNP) is characterized by type 2 inflammation, prominent eosinophil accumulation, and enhanced epithelial\u2013mesenchymal transition (EMT). Although interleukin (IL)-5 plays a central role in eosinophil differentiation and activation, its involvement in driving EMT in human nasal epithelial cells (HNECs) remains unclear. This study investigated IL-5 receptor \u03b1 (IL-5R\u03b1)\u2013mediated signaling and EMT profiles in ECRSwNP, aiming to determine whether IL-5\u2013activated eosinophil-like cells promote EMT in HNECs.Methods: Nasal tissues from healthy controls, non-ECRSwNP patients, and ECRSwNP patients (n=12 per group) were analyzed using hematoxylin and eosin staining, quantitative real-time polymerase chain reaction, immunohistochemistry, and Western blotting. HL-60 promyelocytic leukemia cells were differentiated into eosinophil-like cells and subsequently stimulated with IL-5. HNECs were cocultured with undifferentiated HL-60 cells, differentiated HL-60 cells, or IL-5\u2013stimulated differentiated HL-60 cells using a Transwell system. Expression of EMT-related markers was assessed by Western blotting and immunofluorescence.Results: ECRSwNP tissues demonstrated markedly increased eosinophil infiltration, elevated IL-4 and IL-5 transcript levels, and significantly higher expression of IL-5R\u03b1, phosphorylated Janus kinase 2 (JAK2), and phosphorylated signal transducer and activator of transcription 5 (STAT5) compared with controls and non-ECRSwNP tissues. EMT-associated changes, characterized by reduced E-cadherin and increased vimentin expression, were most pronounced in ECRSwNP. In vitro, IL-5 stimulation enhanced IL-5R\u03b1 expression, activated JAK2\/STAT5 signaling, and increased secretion of eosinophil-associated mediators in differentiated HL-60 cells. Co-culture with IL-5-stimulated eosinophil-like cells induced EMT in HNECs, whereas unstimulated HL-60 cells exerted minimal effects.Conclusion: IL-5R\u03b1-mediated signaling is upregulated in ECRSwNP and may contribute to EMT through eosinophil-dependent mechanisms. IL-5\u2013activated eosinophils promote EMT-related changes in HNECs, providing a mechanistic link between type 2 inflammation and epithelial remodeling. These findings suggest that IL-5R\u03b1 signaling may represent a potential therapeutic target for modulating tissue remodeling in ECRSwNP.<\/jats:p>","DOI":"10.18787\/jr.2025.00065","type":"journal-article","created":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T00:16:08Z","timestamp":1785284168000},"page":"95-106","update-policy":"https:\/\/doi.org\/10.18787\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["IL-5R\u03b1\u2013Mediated Signaling in Eosinophils May Contribute to the Epithelial\u2013Mesenchymal Transition in Eosinophilic Chronic Rhinosinusitis With Nasal Polyps"],"prefix":"10.18787","volume":"33","author":[{"ORCID":"https:\/\/orcid.org\/0009-0000-9871-3495","authenticated-orcid":true,"given":"Hosung","family":"Choi","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea"},{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, Daejeon St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9502-494X","authenticated-orcid":true,"given":"Hyunsu","family":"Choi","sequence":"additional","affiliation":[{"name":"Clinical Research Institute, Daejeon St. Mary\u2019s Hospital, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5553-2549","authenticated-orcid":true,"given":"Jeong-Min","family":"Oh","sequence":"additional","affiliation":[{"name":"Clinical Research Institute, Daejeon St. Mary\u2019s Hospital, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8981-2536","authenticated-orcid":true,"given":"Sung Won","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6917-5998","authenticated-orcid":true,"given":"Soo Whan","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2794-7803","authenticated-orcid":true,"given":"Byung Guk","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2387-3520","authenticated-orcid":true,"given":"Jin Hee","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7917-4390","authenticated-orcid":true,"given":"Dong Chang","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea"},{"name":"Department of Otorhinolaryngology-Head and Neck Surgery, Daejeon St. Mary\u2019s Hospital, College of Medicine, The Catholic University of Korea, Daejeon, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"7989","reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1038\/s41572-020-00218-1","type":"journal-article"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1111\/cea.14592","type":"journal-article"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2025.17.1.8","type":"journal-article"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.3390\/medicina61091620","type":"journal-article"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.18787\/jr.2024.00022","type":"journal-article"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1159\/000524950","type":"journal-article"},{"key":"ref7","doi-asserted-by":"publisher","DOI":"10.1159\/000516847","type":"journal-article"},{"key":"ref8","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.110554","type":"journal-article"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1002\/alr.22994","type":"journal-article"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.4193\/rhino10.261","type":"journal-article"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1111\/all.12644","type":"journal-article"},{"key":"ref12","doi-asserted-by":"publisher","DOI":"10.2183\/pjab.87.463","type":"journal-article"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.3390\/biomedicines12071531","type":"journal-article"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.4168\/aair.2010.2.2.87","type":"journal-article"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.10.6409","type":"journal-article"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2024.1460104","type":"journal-article"},{"key":"ref17","doi-asserted-by":"publisher","DOI":"10.1016\/j.pupt.2024.102286","type":"journal-article"},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1159\/000497829","type":"journal-article"},{"key":"ref19","first-page":"61","type":"journal-article","volume-title":"TGF-\u03b21 induces epithelial-to-mesenchymal transition in chronic rhinosinusitis with nasal polyps through microRNA-182","author":"Jiang","year":"2024"}],"container-title":["Journal of 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