{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T17:44:53Z","timestamp":1791049493901,"version":"4.1.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","issue":"8","license":[{"start":{"date-parts":[[2004,8,1]],"date-time":"2004-08-01T00:00:00Z","timestamp":1091318400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2009,11,5]],"date-time":"2009-11-05T00:00:00Z","timestamp":1257379200000},"content-version":"vor","delay-in-days":1922,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2004,8,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A multigeneration acclimation experiment was performed with Daphnia magna exposed to copper to assess possible changes in tolerance and to establish the optimal concentration range (OCEE) of this species. The hypothesis was tested that as the bioavailable background concentration of an essential metal increases (within realistic limits), the natural tolerance (to the metal) of the acclimated\/adapted organisms and communities will increase. During 18 months the daphnids were exposed to six different, environmentally relevant, copper background concentrations ranging between 0.5 and 100 \u03bcg Cu L\u22121 (7 \u00d7 10\u221215 and 3.7 \u00d7 10\u22129 M Cu2+). An increase in acute (effect concentration resulting in 50% immobility: 48-h EC50) and chronic copper (effect concentration resulting in 50% or 10% reproduction reduction: 21-d EC50, 21-d EC10) tolerance was observed with increasing exposure concentration. The 48-h EC50 increased significantly from 204 \u00b1 24 \u03bcg Cu L\u22121 to 320 \u00b1 43 \u03bcg Cu L\u22121. A nonsignificant change from 48.0 (47.9\u201348.0) \u03bcg Cu L\u22121 to 78.8 (66.3\u201393.6) \u03bcg Cu L\u22121 was noted in the chronic toxicity assays. The optimal concentration range was assessed using different biological parameters (i.e., net reproduction [R0]), energy reserves (Ea), body length measurements, filtration rates, and body burdens. After three generations of acclimation the OCEE ranged between 1 and 35 \u03bcg Cu L\u22121 (2 \u00d7 10\u221214 to 80 \u00d7 10\u221212 M Cu2+). Body burden measurements revealed an active copper regulation up to 35 \u03bcg Cu L\u22121 (80 pM Cu2+). It can be concluded that acclimation of D. magna to copper does occur in laboratory experiments, even at realistic copper background concentrations (10\u221211\u2013 10\u22129 M Cu2+). However, it is suggested that this phenomenon is of less importance in the context of regulatory risk assessments. An optimal copper concentration range for D. magna was observed between 1 and 35 \u03bcg Cu L\u22121 (10\u221214 \u221210\u221211 M Cu2+), indicating that copper deficiency can occur in routine laboratory cultures.<\/jats:p>","DOI":"10.1897\/03-377","type":"journal-article","created":{"date-parts":[[2004,11,18]],"date-time":"2004-11-18T18:24:41Z","timestamp":1100802281000},"page":"2029-2037","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Influence of multigeneration acclimation to copper on tolerance, energy reserves, and homeostasis of <i>Daphnia magna<\/i> Straus"],"prefix":"10.1093","volume":"23","author":[{"given":"Bart T. A.","family":"Bossuyt","sequence":"first","affiliation":[{"name":"Laboratory of Environmental Toxicology and Aquatic Ecology, Ghent University, J. 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