{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T14:47:41Z","timestamp":1777560461293,"version":"3.51.4"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2008,7,1]],"date-time":"2008-07-01T00:00:00Z","timestamp":1214870400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"},{"start":{"date-parts":[[2009,12,9]],"date-time":"2009-12-09T00:00:00Z","timestamp":1260316800000},"content-version":"vor","delay-in-days":526,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2008,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>The aim of the present study was to evaluate oxidative injury in gill pavement cells (GPCs) from fathead minnow (Pimephales promelas) using F2-isoprostane (F2-iP) release as an index of lipid peroxidation. Cells were isolated from pooled gill tissue by collagenase treatment, mechanical sieving, and Percoll\u00ae density gradient centrifugation. Baseline levels of 8-epi-prostaglandin F2\u03b1 (8-epi-PGF2\u03b1) were measured by incubating GPCs in physiological buffer (106 cells\/ml) and enzyme immunoassay. After 60 min, the amount of immunoreactive 8-epi-PGF2\u03b1 (ir8-epi-PGF2\u03b1) in control medium ranged from 1,374 to 5,515 pg\/ml. Lead nitrate, 0.6 to 120 \u03bcM, did not influence ir8-epi-PGF2\u03b1 release, whereas FeCl3 stimulated release at 500 \u03bcM but not at 5 \u03bcM. Incubation medium was extracted for acidic lipids and analyzed by liquid chromatography\/mass spectrometry\/electrosprayionization. A compound in the medium exhibited a retention time on reverse-phase high-performance liquid chromatography nearly identical to that of synthetic 8-epi-PGF2\u03b1 The mass spectrum taken from the total ion chromatogram from 14.8 to 15.1 min contained a prominent ion at m\/z 353, as expected for the molecular ion of 8-epi-PGF2\u03b1. Similar results were obtained with tissue subjected to base hydrolysis. Mass spectra of extracted ion chromatograms obtained with gill extracts and authentic standard showed a close correspondence of fragment ions, providing definitive evidence for production and storage of F2-iPs by fish gills. In summary, F2-iP release occurs during lipid peroxidation injury to fish gill epithelium, and its measurement may facilitate aquatic toxicology studies of metallic and nonmetallic contaminants.<\/jats:p>","DOI":"10.1897\/07-510.1","type":"journal-article","created":{"date-parts":[[2008,4,21]],"date-time":"2008-04-21T16:46:47Z","timestamp":1208796407000},"page":"1569-1575","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Release of the lipid peroxidation marker 8-epi-prostaglandin F2\u03b1 from isolated gill pavement cells"],"prefix":"10.1093","volume":"27","author":[{"given":"Eric G","family":"Spokas","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry, Rider University, Lawrenceville, New Jersey 08648-3099, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Scott","family":"Harshman","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Rowan University, Glassboro, New Jersey 08028-1701, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Glenn M","family":"Cohen","sequence":"additional","affiliation":[{"name":"Department of Biological and Environmental Sciences, Troy University, Troy, Alabama 36082, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chen","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaime M","family":"Levine","sequence":"additional","affiliation":[{"name":"Department of Cell Biology, University of Medicine and Dentistry of New Jersey School of Osteopathic Medicine, Stratford, New Jersey 08084, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana 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