{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,1,4]],"date-time":"2025-01-04T05:34:52Z","timestamp":1735968892545,"version":"3.32.0"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2009,11,1]],"date-time":"2009-11-01T00:00:00Z","timestamp":1257033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2010,1,6]],"date-time":"2010-01-06T00:00:00Z","timestamp":1262736000000},"content-version":"vor","delay-in-days":66,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["setac.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":[],"published-print":{"date-parts":[[2009,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Estuarine sediment microcosms were treated with combinations of diesel, copper (at two levels), and a mixture of heavy metals (mercury, cadmium, lead, and chromium; at two levels) mimicking the contaminant loadings found in harbor sediments. The effects on the microbial community were monitored by polar lipid fatty acid analysis. Diesel addition increased microbial biomass, caused shifts in some fatty acid structural groups, and decreased starvation biomarkers. Incorporation of diesel hydrocarbons into lipids was expressed as an increase in the proportion of odd-carbon-number fatty acids. No treatment with the metals mixture (mercury, cadmium, lead, and chromium) alone significantly changed any parameter derived from the polar lipid fatty acids, but the increase in microbial biomass from diesel addition was higher with the metals mixture, possibly because of indirect effects caused by reductions in grazing resulting from metal-induced toxicity to bacteriovorous nematodes. Copper also modified the effects of diesel addition, preventing biomass increase but not diesel degradation, suggesting that some of the energy gained from diesel oxidation was expended combating copper toxicity. In the present study, observations indicate that metals in general, and copper in particular, can modify the response of sedimentary microorganisms to petroleum-hydrocarbon contaminants.<\/jats:p>","DOI":"10.1897\/08-585.1","type":"journal-article","created":{"date-parts":[[2009,7,6]],"date-time":"2009-07-06T16:45:38Z","timestamp":1246898738000},"page":"2289-2297","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["EFfects of Diesel and Interactions with Copper and Other Metals in an Estuarine Sediment Microbial Community"],"prefix":"10.1093","volume":"28","author":[{"given":"David B.","family":"Hedrick","sequence":"first","affiliation":[{"name":"Center for Biomarker Analysis, University of Tennessee, Knoxville, Tennessee 37996, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aaron D.","family":"Peacock","sequence":"additional","affiliation":[{"name":"Haley and Aldrich, 103 Newhaven Road, Oak Ridge, Tennessee 37830, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guglielmo","family":"Tita","sequence":"additional","affiliation":[{"name":"Universit\u00e9 du Qu\u00e9bec \u00e0 Rimouski (UQAR), \u01cfles-de-la-Madeleine, Quebec G4T 5L9, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John W.","family":"Fleeger","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kevin R.","family":"Carman","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Louisiana State University, Baton Rouge, Louisiana 70803, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David C.","family":"White","sequence":"additional","affiliation":[{"name":"Center for Biomarker Analysis, University of Tennessee, Knoxville, Tennessee 37996, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2009,11,1]]},"reference":[{"key":"2025010317062569200_bib1","doi-asserted-by":"crossref","first-page":"776","DOI":"10.1128\/aem.56.3.776-781.1990","article-title":"Comparison of phenotypic diversity and DNA heterogeneity in a population of soil bacteria","volume":"56","author":"Torsvik","year":"1990","journal-title":"Appl Environ Microbiol"},{"key":"2025010317062569200_bib2","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1021\/es990850i","article-title":"Influence of grazing and nitrogen on benthic algal blooms in diesel fuel-contaminated salt marsh sediments","volume":"34","author":"Carman","year":"2000","journal-title":"Environ Sci Technol"},{"key":"2025010317062569200_bib3","doi-asserted-by":"crossref","first-page":"255","DOI":"10.1016\/S0141-1136(99)00072-0","article-title":"Does historical exposure to hydrocarbon contamination alter the response of benthic communities to diesel contamination?","volume":"49","author":"Carman","year":"2000","journal-title":"Mar Environ Res"},{"key":"2025010317062569200_bib4","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/S0048-9697(03)00141-4","article-title":"Indirect effects of contaminants in aquatic ecosystems","volume":"317","author":"Fleeger","year":"2003","journal-title":"Sci Total Environ"},{"key":"2025010317062569200_bib5","doi-asserted-by":"crossref","first-page":"180","DOI":"10.1016\/j.jembe.2005.12.026","article-title":"Does bioturbation by a benthic fish modify the effects of sediment contamination on salt marsh benthic microalgae and meiofauna?","volume":"330","author":"Fleeger","year":"2006","journal-title":"J Exp Mar Biol Ecol"},{"key":"2025010317062569200_bib6","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1002\/etc.5620200923","article-title":"Linking effects of metal enrichment with metal concentration and speciation in a Louisiana salt marsh meiofaunal community","volume":"20","author":"Millward","year":"2001","journal-title":"Environ Toxicol Chem"},{"key":"2025010317062569200_bib7","doi-asserted-by":"crossref","first-page":"115","DOI":"10.1016\/j.jembe.2004.04.004","article-title":"Mixtures of metals and hydrocarbons elicit complex responses by a benthic invertebrate community","volume":"310","author":"Millward","year":"2004","journal-title":"J Exp Mar Biol Ecol"},{"key":"2025010317062569200_bib8","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/S0167-7012(00)00157-3","article-title":"Measuring microbial community diversity using polar lipid fatty acid and denaturing gradient gel electrophoresis data","volume":"41","author":"Hedrick","year":"2000","journal-title":"J Microbiol Methods"},{"key":"2025010317062569200_bib9","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1139\/f86-007","article-title":"High-density culture of meiobenthic harpacticoid copepods within a muddy sediment substrate","volume":"43","author":"Chandler","year":"1986","journal-title":"Can J Fish Aquat Sci"},{"key":"2025010317062569200_bib10","first-page":"133","volume-title":"Ecology of Estuaries: Anthropogenic Effects","author":"Kennish","year":"1992"},{"key":"2025010317062569200_bib11","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/BF00388810","article-title":"Determination of the sedimentary microbial biomass by extractable lipid phosphate","volume":"40","author":"White","year":"1979","journal-title":"Oecologia"},{"key":"2025010317062569200_bib12","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1111\/j.1574-6968.1985.tb01143.x","article-title":"Phospholipid ester\u2013linked fatty acid profiles as reproducible assays for changes in prokaryotic community structure of estuarine sediments","volume":"31","author":"Guckert","year":"1985","journal-title":"FEMS Microbiol Ecol"},{"key":"2025010317062569200_bib13","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1111\/j.1574-6941.1994.tb00085.x","article-title":"Detection of the anaerobic dechlorinator Desulfomonile tiedjei in soil by its signature lipopolysaccharide branched-long-chain hydroxy fatty acids","volume":"14","author":"Ringelberg","year":"1994","journal-title":"FEMS Microbiol Ecol"},{"key":"2025010317062569200_bib14","doi-asserted-by":"crossref","first-page":"39","DOI":"10.1111\/j.1574-6968.1989.tb03656.x","article-title":"Validation of signature polar lipid fatty acid biomarkers for alkane-utilizing bacteria in soils and subsurface aquifer materials","volume":"62","author":"Ringelberg","year":"1989","journal-title":"FEMS Microbiol Ecol"},{"key":"2025010317062569200_bib15","doi-asserted-by":"crossref","first-page":"18","DOI":"10.1016\/j.jembe.2007.11.012","article-title":"Bacterivory by benthic organisms in sediment: Quantification using 15N-enriched bacteria","volume":"355","author":"Pascal","year":"2008","journal-title":"J Exp Mar Biol Ecol"},{"key":"2025010317062569200_bib16","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1128\/9781555815882.ch10","volume-title":"ASM Manual of Environmental Microbiology","author":"Hedrick","year":"2007","edition":"3rd ed."},{"key":"2025010317062569200_bib17","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1128\/mr.55.2.288-302.1991","article-title":"Iso- and anteiso-fatty acids in bacteria: Biosynthesis, function, and taxonomic significance","volume":"55","author":"Kaneda","year":"1991","journal-title":"Microbiol Rev"},{"key":"2025010317062569200_bib18","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1128\/AEM.71.12.8426-8433.2005","article-title":"Phospholipid furan fatty acids and ubiquinone-8, lipid biomarkers that may protect Dehalococcoides strains from free radicals","volume":"71","author":"White","year":"2005","journal-title":"Appl Environ Microbiol"},{"key":"2025010317062569200_bib19","doi-asserted-by":"crossref","first-page":"76","DOI":"10.1128\/jb.119.1.76-81.1974","article-title":"Influence of hydrocarbons and derivatives on the polar lipid fatty acids of an Acinetobacter isolate","volume":"119","author":"Patrick","year":"1974","journal-title":"J Bacteriol"},{"key":"2025010317062569200_bib20","doi-asserted-by":"crossref","first-page":"5532","DOI":"10.1128\/AEM.65.12.5532-5540.1999","article-title":"Initial reactions in anaerobic alkane degradation by a sulfate reducer, strain AK-01","volume":"65","author":"So","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"2025010317062569200_bib21","first-page":"109","volume-title":"Environmental Chemistry","author":"Farrington","year":"1975"},{"volume-title":"Microbial Lipids","year":"1988","author":"Ratledge","key":"2025010317062569200_bib22"},{"key":"2025010317062569200_bib23","doi-asserted-by":"crossref","first-page":"468","DOI":"10.1007\/s00203-002-0415-5","article-title":"Desulfobulbus mediterraneus sp. nov., a sulfate-reducing bacterium growing on mono- and disaccharides","volume":"177","author":"Sass","year":"2002","journal-title":"Arch Microbiol"},{"key":"2025010317062569200_bib24","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1128\/aem.53.1.4-9.1987","article-title":"Variation in phospholipid ester-linked fatty acids and carotenoids of desiccated Nostoc commune (Cyanobacteria) from different geographic locations","volume":"53","author":"Potts","year":"1987","journal-title":"Appl Environ Microbiol"},{"key":"2025010317062569200_bib25","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1007\/BF02931279","article-title":"Fatty acid composition of six freshwater wild cyanobacterial species","volume":"48","author":"Rezanka","year":"2003","journal-title":"Folia Microbiol"},{"key":"2025010317062569200_bib26","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1007\/s11745-003-1140-7","article-title":"Phospholipid fatty acid profiles of deep-sea piezophilic bacteria from the deep sea","volume":"38","author":"Fang","year":"2003","journal-title":"Lipids"},{"key":"2025010317062569200_bib27","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/S1385-1101(03)00044-3","article-title":"Composition, assimilation and degradation of Phaeocystis globosa\u2013derived fatty acids in the North Sea","volume":"50","author":"Hamm","year":"2003","journal-title":"J Sea Res"},{"key":"2025010317062569200_bib28","first-page":"1179","article-title":"Fatty acids composition of 10 microalgal species","volume":"27","author":"Pratoomyot","year":"2005","journal-title":"Songklanakarin J Sci Technol"},{"key":"2025010317062569200_bib29","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1128\/aem.52.4.794-801.1986","article-title":"Phospholipid, ester-linked fatty acid profile changes during nutrient deprivation of Vibrio cholerae: Increases in the translcis ratio and proportions of cyclopropyl fatty acids","volume":"52","author":"Guckert","year":"1986","journal-title":"Appl Environ Microbiol"},{"key":"2025010317062569200_bib30","doi-asserted-by":"crossref","first-page":"5693","DOI":"10.1128\/JB.181.18.5693-5700.1999","article-title":"Involvement of the cis\/trans isomerase Cti in solvent resistance of Pseudomonas putida DOT-TIE","volume":"181","author":"Junker","year":"1999","journal-title":"J Bacteriol"},{"key":"2025010317062569200_bib31","first-page":"429","article-title":"Cyclopropane ring formation in membrane lipids of bacteria","volume":"61","author":"Grogan","year":"1997","journal-title":"Microbiol Mol Biol Rev"},{"key":"2025010317062569200_bib32","doi-asserted-by":"crossref","first-page":"397","DOI":"10.1080\/09670260010001736001","article-title":"Short-term response of monospecific and natural algal biofilms to copper exposure","volume":"35","author":"Barranguet","year":"2000","journal-title":"Eur J Phycol"},{"key":"2025010317062569200_bib33","doi-asserted-by":"crossref","first-page":"168","DOI":"10.1016\/j.aquatox.2006.12.006","article-title":"A long-term copper exposure on freshwater ecosystem using lotic mesocosms: Primary producer community responses","volume":"81","author":"Roussel","year":"2007","journal-title":"Aquat Toxicol"},{"key":"2025010317062569200_bib34","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1128\/aem.55.8.2002-2009.1989","article-title":"Bacterial community structure and function along a heavy metal gradient","volume":"55","author":"Dean-Ross","year":"1989","journal-title":"Appl Environ Microbiol"},{"key":"2025010317062569200_bib35","doi-asserted-by":"crossref","first-page":"3223","DOI":"10.1128\/AEM.69.6.3223-3230.2003","article-title":"Cultivation-dependent and -independent approaches for determining bacterial diversity in heavy-metal-contaminated soil","volume":"69","author":"Ellis","year":"2003","journal-title":"Appl Environ Microbiol"},{"key":"2025010317062569200_bib36","doi-asserted-by":"crossref","first-page":"853","DOI":"10.1128\/AEM.65.2.853-855.1999","article-title":"Effect of aromatic compounds on cellular fatty acid composition of Rhodococcus opacus","volume":"65","author":"Tsitko","year":"1999","journal-title":"Appl Environ Microbiol"},{"key":"2025010317062569200_bib37","doi-asserted-by":"crossref","first-page":"899","DOI":"10.1016\/S0146-6380(98)00174-0","article-title":"Biogeochemical evidence for microbial community change in a jet fuel hydrocarbons\u2013contaminated aquifer","volume":"29","author":"Fang","year":"1998","journal-title":"Organic Geochemistry"},{"key":"2025010317062569200_bib38","first-page":"775","volume-title":"Microbial Biosystems: New Frontiers. Proceedings of the Eighth International Symposium on Microbial Ecology","author":"Grossman","year":"1999"},{"key":"2025010317062569200_bib39","doi-asserted-by":"crossref","first-page":"2514","DOI":"10.1128\/aem.57.9.2514-2522.1991","article-title":"Microbial populations and hydrocarbon biodegradation potentials in fertilized shoreline sediments affected by the T\/V Exxon Valdez oil spill","volume":"57","author":"Lindstrom","year":"1991","journal-title":"Appl Environ Microbiol"},{"key":"2025010317062569200_bib40","doi-asserted-by":"crossref","first-page":"1011","DOI":"10.1016\/S0038-0717(01)00004-9","article-title":"Soil microbial community responses to dairy manure or ammonium nitrate applications","volume":"33","author":"Peacock","year":"2001","journal-title":"Soil Biol Biochem"}],"container-title":["Environmental Toxicology and Chemistry"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1897%2F08-585.1","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/etc\/article-pdf\/28\/11\/2289\/59853879\/5620281107.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/etc\/article-pdf\/28\/11\/2289\/59853879\/5620281107.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,3]],"date-time":"2025-01-03T17:21:14Z","timestamp":1735924874000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/etc\/article\/28\/11\/2289\/7763965"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,11,1]]},"references-count":40,"journal-issue":{"issue":"11","published-online":{"date-parts":[[2009,11,1]]},"published-print":{"date-parts":[[2009,11,1]]}},"URL":"https:\/\/doi.org\/10.1897\/08-585.1","relation":{},"ISSN":["0730-7268","1552-8618"],"issn-type":[{"type":"print","value":"0730-7268"},{"type":"electronic","value":"1552-8618"}],"subject":[],"published-other":{"date-parts":[[2009,11,1]]},"published":{"date-parts":[[2009,11,1]]},"assertion":[{"value":"2008-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2009-06-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-01-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}