{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:31:46Z","timestamp":1760920306651,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[1982,5,1]],"date-time":"1982-05-01T00:00:00Z","timestamp":389059200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1982,5]]},"abstract":"<jats:p>P<jats:sc>atients receiving<\/jats:sc> freeze\u2010dried skin (FDS) allografts were evaluated for cell\u2010mediated response by a lymphocytotoxicity test. Eleven patients received single or multiple FDS allografts from a donor typed for human leukocyte antigen (HLA). Heparinized blood samples were drawn before the procedure and at 2, 4, and 8 weeks after grafting. Mononuclear cells were isolated by Ficoll\u2010Hypaque gradients. Cell\u2010mediated lymphocytotoxicity tests were conducted, with mononuclear cells from the FDS allograft recipients used as effector cells. Phytohemagglutinin\u2010stimulated blast target cells of the same HLA type as that of the skin donor were labeled with chromium 51. Lympholysis was evaluated by measuring the amount of <jats:sup>51<\/jats:sup>Cr release after 18 hours' incubation with target\/effector cell ratios of 1:100, 1:50, and 1: 25. Negative control wells contained target cells alone. Positive control wells contained target cells and effector cells sensitized <jats:italic>in vitro<\/jats:italic> against the target cells. Viability of effector cells was tested by trypan\u2010blue dye exclusion and response to phytohemagglutinin. None of the experimental blood samples showed <jats:sup>51<\/jats:sup>Cr release significantly greater than that shown by the negative controls. Our findings of no cell\u2010mediated response and the findings of a previous study showing no production of anti\u2010HLA antibody in response to FDS allografts indicate that allogeneic FDS is an immunologically safe material for use in periodontal surgical procedures.<\/jats:p>","DOI":"10.1902\/jop.1982.53.5.325","type":"journal-article","created":{"date-parts":[[2012,3,21]],"date-time":"2012-03-21T15:34:04Z","timestamp":1332344044000},"page":"325-327","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Immunogenicity of Freeze\u2010Dried Skin Allografts in Humans: Cell\u2010Mediated Response"],"prefix":"10.1002","volume":"53","author":[{"suffix":"Jr.","given":"Marlin E.","family":"Gher","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arthur R.","family":"Vernino","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette E.","family":"Maluish","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Douglas M.","family":"Strong","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1982,5]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1982.53.5.325","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:25:22Z","timestamp":1760873122000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1982.53.5.325"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1982,5]]},"references-count":0,"journal-issue":{"issue":"5","published-print":{"date-parts":[[1982,5]]}},"alternative-id":["10.1902\/jop.1982.53.5.325"],"URL":"https:\/\/doi.org\/10.1902\/jop.1982.53.5.325","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1982,5]]}}}