{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:23:14Z","timestamp":1760919794153,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1984,2,1]],"date-time":"1984-02-01T00:00:00Z","timestamp":444441600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1984,2]]},"abstract":"<jats:p>R<jats:sc>ecent studies have<\/jats:sc> demonstrated an association of <jats:italic>Eubacterium<\/jats:italic> sp. with the subgingival microflora of patients with chronic periodontitis. One species, <jats:italic>Eubacterium brachy<\/jats:italic>, was evaluated to determine the possible mechanisms by which this microorganism may contribute to this disease. An extracellular antigen was identified in the culture supernatant which reacted with antibodies in human sera. This antigen was isolated by methanol precipitation and purified by gel filtration. The purified extracellular antigen was reacted <jats:italic>in vitro<\/jats:italic> with <jats:sup>45<\/jats:sup>CaCl<jats:sub>2<\/jats:sub>\u2010labeled fetal rat bone in a bone resorptive bioassay. This antigen was shown to have a molecular weight of 170,000, to share a line of identity with a sonicated preparation of <jats:italic>E. brachy<\/jats:italic> whole cells and to result in increased <jats:sup>45<\/jats:sup>CaCl<jats:sub>2<\/jats:sub> release from fetal rat bones when cultures were exposed to the purified extracellular antigen at concentrations of 10 to 53 <jats:italic>\u03bc<\/jats:italic>g\/ml.<\/jats:p>","DOI":"10.1902\/jop.1984.55.2.93","type":"journal-article","created":{"date-parts":[[2012,3,21]],"date-time":"2012-03-21T11:51:46Z","timestamp":1332330706000},"page":"93-97","source":"Crossref","is-referenced-by-count":5,"title":["<i>Eubacterium brachy<\/i>: Reactivity in <i>In Vitro<\/i> Bone Resorptive Bioassay"],"prefix":"10.1002","volume":"55","author":[{"given":"Jack W.","family":"Vincent","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"William A.","family":"Falkler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"James R.","family":"Heath","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1984,2]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1984.55.2.93","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T09:13:33Z","timestamp":1760865213000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1984.55.2.93"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1984,2]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1984,2]]}},"alternative-id":["10.1902\/jop.1984.55.2.93"],"URL":"https:\/\/doi.org\/10.1902\/jop.1984.55.2.93","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1984,2]]}}}