{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T03:38:34Z","timestamp":1777347514231,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"11S","license":[{"start":{"date-parts":[[1985,11,1]],"date-time":"1985-11-01T00:00:00Z","timestamp":499651200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1985,11]]},"abstract":"<jats:p><jats:sc>In a series of experiments<\/jats:sc>, Golub et al. demonstrated that tetracyclines, but not other antibiotics, can inhibit mammalian collagenases and proposed that this property could be useful in treating diseases, such as periodontal disease (but also included certain medical conditions, e.g., corneal ulcers) characterized by excessive collagen degradation {<jats:italic>JPeriodont Res<\/jats:italic> 1983, 1984 and 1985; <jats:italic>Experientia<\/jats:italic> 1984; <jats:italic>Cornea<\/jats:italic> 1984). One effect was the dramatic reduction of tissue collagenase activity within the gingival crevicular fluid (GCF) of periodontal pockets after administering a standard regimen of a tetracycline (e.g., 200 mg minocycline or 1000 mg tetracycline\/day). The preliminary studies described below determined the effect of (1) low\u2010dose (LD; 40\u201380 mg\/day) orally administered minocycline on GCF collagenase activity and on the subgingival microflora (Exp. I), and (2) tetracyclineloaded monolithic fibers (TF) on collagenase activity <jats:italic>in vitro<\/jats:italic> (Exp. II). In Exp. I, GCF collagenase activity was reduced by 45 to 80% 2 weeks after initiating LD minocycline therapy, an effect that lasted for at least several weeks after stopping drug treatment. No consistent change in the relative proportions of G(+), G(\u2010) and motile subgingival microorganisms was detected as a result of LD treatment suggesting that the reduction in GCF collagenase activity was a direct inhibition of the enzyme by the drug. In Exp. II, 3\u2010 and 6mm lengths of TF <jats:italic>in vitro<\/jats:italic> established tetracycline concentrations in 250 <jats:italic>\u03bc<\/jats:italic>l of 132 <jats:italic>\u03bc<\/jats:italic>g\/ml, from 3\u2010mm lengths, and 265 <jats:italic>\u03bc<\/jats:italic>g\/ml, from 6\u2010mm lengths, after an 18\u2010hour incubation. Although these concentrations were slightly lower than those established in the GCF of individual TF\u2010treated periodontal pockets <jats:italic>in vivo<\/jats:italic> (Goodson et al. 1983, <jats:italic>J Periodontol<\/jats:italic>), the released tetracycline from 3\u2010 and 6\u2010mm TFs inhibited rat polymorphonuclear leukocyte collagenase activity <jats:italic>in vitro<\/jats:italic> by 21 % and 45%, respectively (bacterial collagenase activity was also inhibited <jats:italic>in vitro<\/jats:italic>). These results indicate that TF placed into individual periodontal pockets could maintain a level of tetracycline in GCF that would effectively inhibit tissue (and perhaps also bacterial) collagenases at the lesion site.<\/jats:p>","DOI":"10.1902\/jop.1985.56.11s.93","type":"journal-article","created":{"date-parts":[[2012,3,21]],"date-time":"2012-03-21T11:10:41Z","timestamp":1332328241000},"page":"93-97","source":"Crossref","is-referenced-by-count":59,"title":["Tetracyclines Inhibit Tissue Collagenases: Effects of Ingested Low\u2010Dose and Local Delivery Systems"],"prefix":"10.1002","volume":"56","author":[{"given":"L. M.","family":"Golub","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. M.","family":"Goodson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. M.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. M.","family":"Vidal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T. F.","family":"McNamara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. S.","family":"Ramamurthy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1985,11]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1985.56.11s.93","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T11:35:49Z","timestamp":1760873749000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1985.56.11s.93"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1985,11]]},"references-count":0,"journal-issue":{"issue":"11S","published-print":{"date-parts":[[1985,11]]}},"alternative-id":["10.1902\/jop.1985.56.11s.93"],"URL":"https:\/\/doi.org\/10.1902\/jop.1985.56.11s.93","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"value":"0022-3492","type":"print"},{"value":"1943-3670","type":"electronic"}],"subject":[],"published":{"date-parts":[[1985,11]]}}}