{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T00:43:56Z","timestamp":1760921036225,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[1990,10,1]],"date-time":"1990-10-01T00:00:00Z","timestamp":654739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1990,10]]},"abstract":"<jats:p>B <jats:sc>lymphocytes and plasma cells<\/jats:sc> are histologically observed in granulomatous periodontal tissues of juvenile periodontitis (JP) patients. Local immune processes may participate in protective or immunopathologic roles in the pathogenesis of this disease. An in vitro expiant culture system was utilized to demonstrate the production of Immunoglobulins by diseased JP tissues. Immunodiffusion studies using goat anti\u2010human gamma, alpha, or mu chain serum revealed IgG to be the major Immunoglobulin present in 92% of the day 1 supernatant fluids (SF) of the 47 JP gingival tissue expiant cultures. IgA was present in 15% of the SF; however, no IgM was detected. Staph Protein A isolated <jats:sup>14<\/jats:sup>C\u2010labeled IgG from the SF, when allowed to react with goat anti\u2010human gamma chain serum, formed lines of precipitation. Positive autoradiographs confirmed the biosynthesis of IgG by the expiant cultures. The in vitro gingival tissue expiant culture system described provides a useful model for the study of localized Immunoglobulins produced by diseased tissues of JP patients. <jats:italic>J Periodontol 1990;61:603\u2013608<\/jats:italic>.<\/jats:p>","DOI":"10.1902\/jop.1990.61.10.603","type":"journal-article","created":{"date-parts":[[2012,3,21]],"date-time":"2012-03-21T06:22:26Z","timestamp":1332310946000},"page":"603-608","source":"Crossref","is-referenced-by-count":4,"title":["Production of Immunoglobulins in Gingival Tissue Explant Cultures From Juvenile Periodontitis Patients"],"prefix":"10.1002","volume":"61","author":[{"given":"Eric R.","family":"Hall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"William A.","family":"Falkler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon B.","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1990,10]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1990.61.10.603","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T14:05:06Z","timestamp":1760882706000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1990.61.10.603"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,10]]},"references-count":0,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1990,10]]}},"alternative-id":["10.1902\/jop.1990.61.10.603"],"URL":"https:\/\/doi.org\/10.1902\/jop.1990.61.10.603","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1990,10]]}}}