{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T11:01:52Z","timestamp":1762340512999,"version":"build-2065373602"},"reference-count":0,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[1991,12,1]],"date-time":"1991-12-01T00:00:00Z","timestamp":691545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Periodontology"],"published-print":{"date-parts":[[1991,12]]},"abstract":"<jats:p>T<jats:sc>he established and advanced lesions<\/jats:sc> of juvenile periodontitis\u2010localized form (JP) are predominated by B\u2010lymphocytes and plasma cells. Local immune processes may participate in protective or immunopathologic roles in the pathogenesis of this disease. <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic> (A.a.) is implicated as a primary etiologic agent in JP. An in vitro gingival explant culture system was utilized to study the specificity of Immunoglobulins produced by diseased JP tissues. A dot\u2010immunobinding assay demonstrated that 46% of the supernatant fluids (SF) from expiant cultures of diseased tissues (n = 39) were positive for the presence of antibody to A.a. Y4, while 61% of autologous JP sera (n = 39) tested positive. For rapidly progressive (RP) and adult periodontitis (AP) SF, 50% and 40% were positive for A.a. Y4, respectively. Seventeen percent of SF from healthy tissue were positive for A.a. Y4. There was no significant difference between JP SF reactivities to A.a. Y4 when compared to reactivities of SF from AP and RP patients. Only 10% of JP SF were positive for <jats:italic>Porphyromonas asaccharolytica<\/jats:italic>, a non\u2010oral control microorganism. The de novo biosynthesis of antibody in JP tissue, reactive with A.a. Y4, was demonstrated with Staph Protein A isolated <jats:sup>14<\/jats:sup>C\u2010labeled IgG (SPAG) and the use of a dot\u2010immunobinding assay and autoradiography. The in vitro gingival tissue expiant culture system described provides a useful model for the study of the synthesis and specificity of localized Immunoglobulins produced by diseased tissues of JP patients. <jats:italic>J Periodontol 1991; 62:792\u2013798.<\/jats:italic><\/jats:p>","DOI":"10.1902\/jop.1991.62.12.792","type":"journal-article","created":{"date-parts":[[2012,3,21]],"date-time":"2012-03-21T04:21:38Z","timestamp":1332303698000},"page":"792-798","source":"Crossref","is-referenced-by-count":6,"title":["The Gingival Immune Response to <i>Actinobacillus actinomycetemcomitans<\/i> in Juvenile Periodontitis"],"prefix":"10.1002","volume":"62","author":[{"given":"Eric R.","family":"Hall","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"William A.","family":"Falkler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen A.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon B.","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1991,12]]},"container-title":["Journal of Periodontology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/pdf\/10.1902\/jop.1991.62.12.792","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T12:42:00Z","timestamp":1760877720000},"score":1,"resource":{"primary":{"URL":"https:\/\/aap.onlinelibrary.wiley.com\/doi\/10.1902\/jop.1991.62.12.792"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,12]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1991,12]]}},"alternative-id":["10.1902\/jop.1991.62.12.792"],"URL":"https:\/\/doi.org\/10.1902\/jop.1991.62.12.792","archive":["Portico"],"relation":{},"ISSN":["0022-3492","1943-3670"],"issn-type":[{"type":"print","value":"0022-3492"},{"type":"electronic","value":"1943-3670"}],"subject":[],"published":{"date-parts":[[1991,12]]}}}